Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_97 and Pf1N1B4_98 are separated by 535 nucleotides Pf1N1B4_98 and Pf1N1B4_99 are separated by 24 nucleotides
Pf1N1B4_97 - Selenide,water dikinase (EC 2.7.9.3), at 101,961 to 102,995
_97
Pf1N1B4_98 - Nucleoside-specific channel-forming protein Tsx precursor, at 103,531 to 104,487
_98
Pf1N1B4_99 - Purine nucleoside permease, at 104,512 to 105,546
_99
Position (kb) 103
104
105 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 102.722 kb on - strand, within Pf1N1B4_97 at 102.722 kb on - strand, within Pf1N1B4_97 at 102.722 kb on - strand, within Pf1N1B4_97 at 102.835 kb on + strand, within Pf1N1B4_97 at 102.836 kb on - strand, within Pf1N1B4_97 at 102.836 kb on - strand, within Pf1N1B4_97 at 102.836 kb on - strand, within Pf1N1B4_97 at 102.836 kb on - strand, within Pf1N1B4_97 at 103.032 kb on + strand at 103.033 kb on - strand at 103.076 kb on - strand at 103.225 kb on - strand at 103.379 kb on - strand at 103.398 kb on - strand at 103.529 kb on + strand at 103.705 kb on + strand, within Pf1N1B4_98 at 103.789 kb on + strand, within Pf1N1B4_98 at 103.798 kb on + strand, within Pf1N1B4_98 at 103.798 kb on + strand, within Pf1N1B4_98 at 103.799 kb on - strand, within Pf1N1B4_98 at 103.804 kb on + strand, within Pf1N1B4_98 at 103.804 kb on + strand, within Pf1N1B4_98 at 103.804 kb on + strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand at 103.834 kb on + strand, within Pf1N1B4_98 at 103.993 kb on + strand, within Pf1N1B4_98 at 103.993 kb on + strand, within Pf1N1B4_98 at 103.993 kb on + strand at 103.993 kb on + strand, within Pf1N1B4_98 at 103.994 kb on - strand, within Pf1N1B4_98 at 103.994 kb on - strand, within Pf1N1B4_98 at 104.014 kb on + strand, within Pf1N1B4_98 at 104.014 kb on + strand, within Pf1N1B4_98 at 104.015 kb on - strand, within Pf1N1B4_98 at 104.015 kb on - strand, within Pf1N1B4_98 at 104.056 kb on + strand, within Pf1N1B4_98 at 104.056 kb on + strand at 104.056 kb on + strand, within Pf1N1B4_98 at 104.057 kb on - strand, within Pf1N1B4_98 at 104.116 kb on + strand, within Pf1N1B4_98 at 104.159 kb on - strand, within Pf1N1B4_98 at 104.191 kb on + strand, within Pf1N1B4_98 at 104.191 kb on + strand, within Pf1N1B4_98 at 104.191 kb on + strand at 104.192 kb on - strand, within Pf1N1B4_98 at 104.192 kb on - strand, within Pf1N1B4_98 at 104.192 kb on - strand, within Pf1N1B4_98 at 104.192 kb on - strand, within Pf1N1B4_98 at 104.192 kb on - strand, within Pf1N1B4_98 at 104.236 kb on + strand, within Pf1N1B4_98 at 104.236 kb on + strand, within Pf1N1B4_98 at 104.236 kb on + strand, within Pf1N1B4_98 at 104.236 kb on + strand, within Pf1N1B4_98 at 104.237 kb on - strand, within Pf1N1B4_98 at 104.332 kb on + strand, within Pf1N1B4_98 at 104.461 kb on + strand at 104.485 kb on + strand at 104.485 kb on + strand at 104.512 kb on - strand at 104.715 kb on + strand, within Pf1N1B4_99 at 104.830 kb on + strand, within Pf1N1B4_99 at 104.902 kb on + strand, within Pf1N1B4_99 at 104.902 kb on + strand, within Pf1N1B4_99 at 104.903 kb on - strand, within Pf1N1B4_99 at 104.903 kb on - strand at 105.020 kb on - strand, within Pf1N1B4_99 at 105.192 kb on + strand, within Pf1N1B4_99 at 105.192 kb on + strand, within Pf1N1B4_99 at 105.255 kb on + strand, within Pf1N1B4_99 at 105.268 kb on + strand, within Pf1N1B4_99 at 105.268 kb on + strand, within Pf1N1B4_99 at 105.269 kb on - strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.302 kb on - strand at 105.302 kb on - strand, within Pf1N1B4_99 at 105.428 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 102,722 - Pf1N1B4_97 0.74 +0.8 102,722 - Pf1N1B4_97 0.74 +0.2 102,722 - Pf1N1B4_97 0.74 -0.6 102,835 + Pf1N1B4_97 0.84 +1.8 102,836 - Pf1N1B4_97 0.85 +0.8 102,836 - Pf1N1B4_97 0.85 -0.2 102,836 - Pf1N1B4_97 0.85 +1.0 102,836 - Pf1N1B4_97 0.85 +2.1 103,032 + -0.2 103,033 - -1.2 103,076 - -0.7 103,225 - +1.1 103,379 - +0.4 103,398 - +0.8 103,529 + -1.2 103,705 + Pf1N1B4_98 0.18 -0.6 103,789 + Pf1N1B4_98 0.27 +0.6 103,798 + Pf1N1B4_98 0.28 +0.8 103,798 + Pf1N1B4_98 0.28 -1.8 103,799 - Pf1N1B4_98 0.28 +0.3 103,804 + Pf1N1B4_98 0.29 -1.2 103,804 + Pf1N1B4_98 0.29 +0.8 103,804 + Pf1N1B4_98 0.29 -1.2 103,805 - Pf1N1B4_98 0.29 +1.6 103,805 - Pf1N1B4_98 0.29 +0.2 103,805 - Pf1N1B4_98 0.29 +1.4 103,805 - Pf1N1B4_98 0.29 -1.2 103,805 - -0.2 103,834 + Pf1N1B4_98 0.32 -0.6 103,993 + Pf1N1B4_98 0.48 -0.2 103,993 + Pf1N1B4_98 0.48 -0.2 103,993 + -0.2 103,993 + Pf1N1B4_98 0.48 -0.2 103,994 - Pf1N1B4_98 0.48 +2.3 103,994 - Pf1N1B4_98 0.48 -0.2 104,014 + Pf1N1B4_98 0.50 +0.8 104,014 + Pf1N1B4_98 0.50 -0.2 104,015 - Pf1N1B4_98 0.51 -1.2 104,015 - Pf1N1B4_98 0.51 -0.9 104,056 + Pf1N1B4_98 0.55 -0.2 104,056 + -0.2 104,056 + Pf1N1B4_98 0.55 +0.8 104,057 - Pf1N1B4_98 0.55 +1.6 104,116 + Pf1N1B4_98 0.61 -1.2 104,159 - Pf1N1B4_98 0.66 +0.6 104,191 + Pf1N1B4_98 0.69 -0.5 104,191 + Pf1N1B4_98 0.69 +0.4 104,191 + -0.2 104,192 - Pf1N1B4_98 0.69 +0.8 104,192 - Pf1N1B4_98 0.69 +0.2 104,192 - Pf1N1B4_98 0.69 +0.8 104,192 - Pf1N1B4_98 0.69 +1.8 104,192 - Pf1N1B4_98 0.69 -0.2 104,236 + Pf1N1B4_98 0.74 -0.2 104,236 + Pf1N1B4_98 0.74 +2.6 104,236 + Pf1N1B4_98 0.74 +2.1 104,236 + Pf1N1B4_98 0.74 +1.1 104,237 - Pf1N1B4_98 0.74 -2.2 104,332 + Pf1N1B4_98 0.84 +0.8 104,461 + -0.8 104,485 + -0.2 104,485 + -0.0 104,512 - +0.4 104,715 + Pf1N1B4_99 0.20 +1.6 104,830 + Pf1N1B4_99 0.31 -0.2 104,902 + Pf1N1B4_99 0.38 +1.0 104,902 + Pf1N1B4_99 0.38 +0.2 104,903 - Pf1N1B4_99 0.38 -1.2 104,903 - -1.8 105,020 - Pf1N1B4_99 0.49 +0.8 105,192 + Pf1N1B4_99 0.66 +0.0 105,192 + Pf1N1B4_99 0.66 +1.4 105,255 + Pf1N1B4_99 0.72 -0.6 105,268 + Pf1N1B4_99 0.73 +2.8 105,268 + Pf1N1B4_99 0.73 +0.8 105,269 - Pf1N1B4_99 0.73 +0.8 105,301 + Pf1N1B4_99 0.76 +0.2 105,301 + Pf1N1B4_99 0.76 +1.4 105,301 + Pf1N1B4_99 0.76 +1.1 105,301 + Pf1N1B4_99 0.76 -1.2 105,301 + Pf1N1B4_99 0.76 +1.8 105,302 - -1.2 105,302 - Pf1N1B4_99 0.76 -0.6 105,428 - -0.2
Or see this region's nucleotide sequence