Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5174 and Pf1N1B4_628 are separated by 63 nucleotides Pf1N1B4_628 and Pf1N1B4_629 are separated by 13 nucleotides Pf1N1B4_629 and Pf1N1B4_630 are separated by 166 nucleotides
Pf1N1B4_5174 - tRNA-Lys-TTT, at 725,744 to 725,816
_5174
Pf1N1B4_628 - Queuosine Biosynthesis QueC ATPase, at 725,880 to 726,560
_628
Pf1N1B4_629 - Queuosine Biosynthesis QueE Radical SAM, at 726,574 to 727,221
_629
Pf1N1B4_630 - TPR repeat containing exported protein; Putative periplasmic protein contains a protein prenylyltransferase domain, at 727,388 to 728,152
_630
Position (kb)
726
727
728 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 725.576 kb on - strand at 725.576 kb on - strand at 725.576 kb on - strand at 725.715 kb on - strand at 725.715 kb on - strand at 725.715 kb on - strand at 725.715 kb on - strand at 725.887 kb on + strand at 725.887 kb on + strand at 725.955 kb on - strand, within Pf1N1B4_628 at 726.096 kb on + strand, within Pf1N1B4_628 at 726.097 kb on - strand, within Pf1N1B4_628 at 726.097 kb on - strand, within Pf1N1B4_628 at 726.176 kb on - strand, within Pf1N1B4_628 at 726.176 kb on - strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.275 kb on - strand, within Pf1N1B4_628 at 726.275 kb on - strand, within Pf1N1B4_628 at 726.275 kb on - strand, within Pf1N1B4_628 at 726.320 kb on + strand, within Pf1N1B4_628 at 726.320 kb on + strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.446 kb on - strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.464 kb on - strand, within Pf1N1B4_628 at 726.464 kb on - strand, within Pf1N1B4_628 at 726.602 kb on + strand at 726.602 kb on + strand at 726.602 kb on + strand at 726.603 kb on - strand at 726.603 kb on - strand at 726.603 kb on - strand at 726.603 kb on - strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.761 kb on + strand, within Pf1N1B4_629 at 726.791 kb on - strand, within Pf1N1B4_629 at 727.019 kb on + strand, within Pf1N1B4_629 at 727.020 kb on - strand, within Pf1N1B4_629 at 727.221 kb on + strand at 727.221 kb on + strand at 727.243 kb on + strand at 727.243 kb on + strand at 727.243 kb on + strand at 727.244 kb on - strand at 727.244 kb on - strand at 727.318 kb on - strand at 727.389 kb on + strand at 727.389 kb on + strand at 727.389 kb on + strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.519 kb on - strand, within Pf1N1B4_630 at 727.740 kb on + strand at 727.740 kb on + strand, within Pf1N1B4_630 at 727.740 kb on + strand, within Pf1N1B4_630 at 727.740 kb on + strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.744 kb on - strand, within Pf1N1B4_630 at 727.744 kb on - strand, within Pf1N1B4_630 at 727.744 kb on - strand, within Pf1N1B4_630 at 727.744 kb on - strand, within Pf1N1B4_630 at 727.867 kb on + strand, within Pf1N1B4_630 at 727.901 kb on + strand, within Pf1N1B4_630 at 727.902 kb on - strand, within Pf1N1B4_630 at 728.077 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.129 kb on - strand at 728.129 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 725,576 - -0.4 725,576 - -1.0 725,576 - -0.0 725,715 - -2.6 725,715 - -1.0 725,715 - +1.6 725,715 - +1.9 725,887 + +1.0 725,887 + +0.4 725,955 - Pf1N1B4_628 0.11 +1.0 726,096 + Pf1N1B4_628 0.32 +0.7 726,097 - Pf1N1B4_628 0.32 +1.0 726,097 - Pf1N1B4_628 0.32 -0.7 726,176 - Pf1N1B4_628 0.43 -0.6 726,176 - Pf1N1B4_628 0.43 +1.0 726,274 + Pf1N1B4_628 0.58 -0.4 726,274 + Pf1N1B4_628 0.58 -0.6 726,274 + Pf1N1B4_628 0.58 +1.6 726,274 + Pf1N1B4_628 0.58 -1.0 726,275 - Pf1N1B4_628 0.58 -0.0 726,275 - Pf1N1B4_628 0.58 -0.6 726,275 - Pf1N1B4_628 0.58 -0.0 726,320 + Pf1N1B4_628 0.65 -1.6 726,320 + Pf1N1B4_628 0.65 -0.4 726,321 - Pf1N1B4_628 0.65 -1.0 726,321 - Pf1N1B4_628 0.65 -0.4 726,321 - Pf1N1B4_628 0.65 +1.0 726,321 - Pf1N1B4_628 0.65 -1.6 726,321 - Pf1N1B4_628 0.65 +1.0 726,321 - Pf1N1B4_628 0.65 +0.6 726,446 - Pf1N1B4_628 0.83 -0.0 726,463 + Pf1N1B4_628 0.86 -1.0 726,463 + Pf1N1B4_628 0.86 -1.0 726,463 + Pf1N1B4_628 0.86 -0.0 726,464 - Pf1N1B4_628 0.86 -0.0 726,464 - Pf1N1B4_628 0.86 -0.6 726,602 + -1.8 726,602 + +1.0 726,602 + -1.0 726,603 - -1.0 726,603 - -0.0 726,603 - +2.3 726,603 - -0.0 726,606 + -1.3 726,606 + +1.6 726,606 + -0.4 726,606 + -0.0 726,606 + +0.7 726,606 + -1.6 726,607 - +1.4 726,607 - -0.0 726,607 - -1.3 726,607 - +1.4 726,607 - +2.2 726,607 - -0.0 726,607 - -1.0 726,607 - +2.3 726,607 - -0.0 726,607 - -1.0 726,607 - -0.6 726,761 + Pf1N1B4_629 0.29 -1.0 726,791 - Pf1N1B4_629 0.33 -1.1 727,019 + Pf1N1B4_629 0.69 -1.0 727,020 - Pf1N1B4_629 0.69 -1.6 727,221 + +0.6 727,221 + +1.0 727,243 + -0.0 727,243 + +0.4 727,243 + +0.6 727,244 - +1.0 727,244 - -0.6 727,318 - +0.6 727,389 + +1.0 727,389 + -0.0 727,389 + -0.4 727,390 - -0.0 727,390 - +2.8 727,390 - +0.2 727,390 - -0.0 727,390 - +2.6 727,390 - +0.6 727,390 - +3.0 727,390 - -1.0 727,519 - Pf1N1B4_630 0.17 +1.3 727,740 + -0.0 727,740 + Pf1N1B4_630 0.46 +2.0 727,740 + Pf1N1B4_630 0.46 -0.0 727,740 + Pf1N1B4_630 0.46 -1.0 727,741 - Pf1N1B4_630 0.46 +1.6 727,741 - Pf1N1B4_630 0.46 -1.0 727,741 - Pf1N1B4_630 0.46 +2.7 727,741 - Pf1N1B4_630 0.46 +1.6 727,741 - Pf1N1B4_630 0.46 -1.6 727,741 - Pf1N1B4_630 0.46 +0.7 727,744 - Pf1N1B4_630 0.47 -0.6 727,744 - Pf1N1B4_630 0.47 +0.3 727,744 - Pf1N1B4_630 0.47 +0.3 727,744 - Pf1N1B4_630 0.47 +1.0 727,867 + Pf1N1B4_630 0.63 -0.0 727,901 + Pf1N1B4_630 0.67 -0.8 727,902 - Pf1N1B4_630 0.67 +2.0 728,077 - -1.0 728,114 - -1.6 728,114 - -1.6 728,114 - +1.0 728,114 - -1.0 728,114 - -0.0 728,114 - -0.0 728,114 - -1.0 728,129 - -1.6 728,129 - -2.6 728,205 - +1.0 728,205 - +1.3 728,205 - -1.0 728,205 - +0.1 728,205 - -0.6 728,205 - -0.0
Or see this region's nucleotide sequence