Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5831 and Pf1N1B4_5832 are separated by 144 nucleotides Pf1N1B4_5832 and Pf1N1B4_5833 are separated by 243 nucleotides Pf1N1B4_5833 and Pf1N1B4_5834 are separated by 36 nucleotides
Pf1N1B4_5831 - SAM-dependent methyltransferases, at 265,508 to 266,557
_5831
Pf1N1B4_5832 - LysR family transcriptional regulator near succinyl-CoA:3-ketoacid-coenzyme A transferase, at 266,702 to 267,601
_5832
Pf1N1B4_5833 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5), at 267,845 to 268,543
_5833
Pf1N1B4_5834 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5), at 268,580 to 269,245
_5834
Position (kb)
266
267
268 Strain fitness (log2 ratio)
-1
0
1
2 at 265.802 kb on + strand, within Pf1N1B4_5831 at 265.802 kb on + strand, within Pf1N1B4_5831 at 265.950 kb on - strand, within Pf1N1B4_5831 at 266.108 kb on + strand, within Pf1N1B4_5831 at 266.217 kb on - strand, within Pf1N1B4_5831 at 266.231 kb on + strand at 266.231 kb on + strand, within Pf1N1B4_5831 at 266.285 kb on + strand, within Pf1N1B4_5831 at 266.286 kb on - strand, within Pf1N1B4_5831 at 266.381 kb on + strand, within Pf1N1B4_5831 at 266.382 kb on - strand, within Pf1N1B4_5831 at 266.398 kb on + strand, within Pf1N1B4_5831 at 266.399 kb on - strand, within Pf1N1B4_5831 at 266.552 kb on - strand at 266.556 kb on - strand at 267.026 kb on - strand, within Pf1N1B4_5832 at 267.120 kb on + strand, within Pf1N1B4_5832 at 267.121 kb on - strand, within Pf1N1B4_5832 at 267.173 kb on + strand, within Pf1N1B4_5832 at 267.831 kb on + strand at 267.832 kb on - strand at 267.832 kb on - strand at 267.832 kb on - strand at 267.832 kb on - strand at 267.844 kb on + strand at 267.845 kb on - strand at 267.845 kb on - strand at 267.870 kb on - strand at 268.083 kb on - strand, within Pf1N1B4_5833 at 268.275 kb on - strand, within Pf1N1B4_5833 at 268.478 kb on + strand at 268.478 kb on + strand at 268.478 kb on + strand at 268.478 kb on + strand at 268.479 kb on - strand at 268.479 kb on - strand at 268.572 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 265,802 + Pf1N1B4_5831 0.28 +1.1 265,802 + Pf1N1B4_5831 0.28 +0.4 265,950 - Pf1N1B4_5831 0.42 +0.3 266,108 + Pf1N1B4_5831 0.57 +1.4 266,217 - Pf1N1B4_5831 0.68 -0.2 266,231 + -1.2 266,231 + Pf1N1B4_5831 0.69 +0.6 266,285 + Pf1N1B4_5831 0.74 -0.2 266,286 - Pf1N1B4_5831 0.74 +0.5 266,381 + Pf1N1B4_5831 0.83 -0.6 266,382 - Pf1N1B4_5831 0.83 +0.2 266,398 + Pf1N1B4_5831 0.85 -0.2 266,399 - Pf1N1B4_5831 0.85 +2.4 266,552 - -0.8 266,556 - +0.4 267,026 - Pf1N1B4_5832 0.36 +0.5 267,120 + Pf1N1B4_5832 0.46 -1.2 267,121 - Pf1N1B4_5832 0.47 -0.2 267,173 + Pf1N1B4_5832 0.52 -0.2 267,831 + +0.8 267,832 - +2.0 267,832 - +1.4 267,832 - -0.2 267,832 - +1.4 267,844 + -0.8 267,845 - -0.2 267,845 - -0.2 267,870 - +0.8 268,083 - Pf1N1B4_5833 0.34 +1.7 268,275 - Pf1N1B4_5833 0.62 +1.4 268,478 + -0.8 268,478 + +0.8 268,478 + +2.1 268,478 + +0.5 268,479 - -0.2 268,479 - -1.2 268,572 + -0.8
Or see this region's nucleotide sequence