Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5684 and Pf1N1B4_5685 are separated by 7 nucleotides Pf1N1B4_5685 and Pf1N1B4_5686 are separated by 2 nucleotides Pf1N1B4_5686 and Pf1N1B4_5687 are separated by 132 nucleotides Pf1N1B4_5687 and Pf1N1B4_5688 are separated by 57 nucleotides Pf1N1B4_5688 and Pf1N1B4_5689 are separated by 11 nucleotides
Pf1N1B4_5684 - Glyceraldehyde-3-phosphate dehydrogenase, putative, at 123,711 to 125,336
_5684
Pf1N1B4_5685 - Permease of the drug/metabolite transporter (DMT) superfamily, at 125,344 to 125,835
_5685
Pf1N1B4_5686 - Permease of the drug/metabolite transporter (DMT) superfamily, at 125,838 to 126,239
_5686
Pf1N1B4_5687 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 126,372 to 126,809
_5687
Pf1N1B4_5688 - hypothetical protein, at 126,867 to 127,196
_5688
Pf1N1B4_5689 - Lactoylglutathione lyase, at 127,208 to 127,600
_5689
Position (kb)
125
126
127 Strain fitness (log2 ratio)
-2
-1
0
1 at 125.016 kb on + strand, within Pf1N1B4_5684 at 125.017 kb on - strand, within Pf1N1B4_5684 at 125.158 kb on - strand, within Pf1N1B4_5684 at 125.326 kb on - strand at 125.558 kb on + strand, within Pf1N1B4_5685 at 125.558 kb on + strand, within Pf1N1B4_5685 at 125.558 kb on + strand, within Pf1N1B4_5685 at 125.558 kb on + strand at 125.558 kb on + strand, within Pf1N1B4_5685 at 125.559 kb on - strand at 125.559 kb on - strand, within Pf1N1B4_5685 at 125.559 kb on - strand at 125.559 kb on - strand, within Pf1N1B4_5685 at 125.784 kb on - strand, within Pf1N1B4_5685 at 125.935 kb on + strand, within Pf1N1B4_5686 at 125.936 kb on - strand, within Pf1N1B4_5686 at 126.222 kb on + strand at 126.223 kb on - strand at 126.250 kb on + strand at 126.251 kb on - strand at 126.522 kb on + strand, within Pf1N1B4_5687 at 126.523 kb on - strand, within Pf1N1B4_5687 at 126.834 kb on - strand at 126.901 kb on + strand, within Pf1N1B4_5688 at 127.005 kb on + strand, within Pf1N1B4_5688 at 127.005 kb on + strand, within Pf1N1B4_5688 at 127.005 kb on + strand, within Pf1N1B4_5688 at 127.006 kb on - strand at 127.020 kb on + strand, within Pf1N1B4_5688 at 127.068 kb on + strand, within Pf1N1B4_5688 at 127.069 kb on - strand, within Pf1N1B4_5688 at 127.074 kb on + strand at 127.074 kb on + strand, within Pf1N1B4_5688 at 127.075 kb on - strand, within Pf1N1B4_5688 at 127.075 kb on - strand, within Pf1N1B4_5688 at 127.096 kb on + strand, within Pf1N1B4_5688 at 127.097 kb on - strand, within Pf1N1B4_5688 at 127.109 kb on - strand, within Pf1N1B4_5688 at 127.155 kb on + strand, within Pf1N1B4_5688
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 125,016 + Pf1N1B4_5684 0.80 -0.2 125,017 - Pf1N1B4_5684 0.80 +0.7 125,158 - Pf1N1B4_5684 0.89 +1.1 125,326 - -0.3 125,558 + Pf1N1B4_5685 0.43 -0.9 125,558 + Pf1N1B4_5685 0.43 +0.1 125,558 + Pf1N1B4_5685 0.43 -0.3 125,558 + -0.3 125,558 + Pf1N1B4_5685 0.43 -0.9 125,559 - -0.3 125,559 - Pf1N1B4_5685 0.44 +1.3 125,559 - -0.3 125,559 - Pf1N1B4_5685 0.44 -0.3 125,784 - Pf1N1B4_5685 0.89 +1.3 125,935 + Pf1N1B4_5686 0.24 -2.3 125,936 - Pf1N1B4_5686 0.24 -0.3 126,222 + -1.9 126,223 - -0.3 126,250 + -0.3 126,251 - -0.3 126,522 + Pf1N1B4_5687 0.34 -1.3 126,523 - Pf1N1B4_5687 0.34 -0.7 126,834 - -1.3 126,901 + Pf1N1B4_5688 0.10 +0.3 127,005 + Pf1N1B4_5688 0.42 -0.3 127,005 + Pf1N1B4_5688 0.42 -0.3 127,005 + Pf1N1B4_5688 0.42 +1.5 127,006 - +0.7 127,020 + Pf1N1B4_5688 0.46 -2.6 127,068 + Pf1N1B4_5688 0.61 -0.3 127,069 - Pf1N1B4_5688 0.61 +1.1 127,074 + +0.7 127,074 + Pf1N1B4_5688 0.63 +1.3 127,075 - Pf1N1B4_5688 0.63 -1.9 127,075 - Pf1N1B4_5688 0.63 +0.3 127,096 + Pf1N1B4_5688 0.69 -0.8 127,097 - Pf1N1B4_5688 0.70 -1.5 127,109 - Pf1N1B4_5688 0.73 -0.4 127,155 + Pf1N1B4_5688 0.87 -1.3
Or see this region's nucleotide sequence