Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_560 and Pf1N1B4_561 are separated by 99 nucleotides Pf1N1B4_561 and Pf1N1B4_562 are separated by 143 nucleotides Pf1N1B4_562 and Pf1N1B4_563 overlap by 4 nucleotides Pf1N1B4_563 and Pf1N1B4_564 are separated by 233 nucleotides
Pf1N1B4_560 - Transcriptional regulators, LysR family, at 657,019 to 657,951
_560
Pf1N1B4_561 - Glutathione S-transferase (EC 2.5.1.18), at 658,051 to 658,653
_561
Pf1N1B4_562 - Histidine transporter, periplasmic histidine-binding protein, at 658,797 to 659,810
_562
Pf1N1B4_563 - RNA polymerase sigma-70 factor, at 659,807 to 660,355
_563
Pf1N1B4_564 - ABC-type phosphate/phosphonate transport system, periplasmic component, at 660,589 to 661,353
_564
Position (kb) 658
659
660 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 657.957 kb on + strand at 657.958 kb on - strand at 658.013 kb on + strand at 658.013 kb on + strand at 658.013 kb on + strand at 658.014 kb on - strand at 658.060 kb on + strand at 658.060 kb on + strand at 658.060 kb on + strand at 658.060 kb on + strand at 658.060 kb on + strand at 658.060 kb on + strand at 658.060 kb on + strand at 658.061 kb on - strand at 658.061 kb on - strand at 658.061 kb on - strand at 658.061 kb on - strand at 658.061 kb on - strand at 658.264 kb on + strand, within Pf1N1B4_561 at 658.265 kb on - strand, within Pf1N1B4_561 at 658.483 kb on + strand, within Pf1N1B4_561 at 658.483 kb on + strand at 658.483 kb on + strand at 658.483 kb on + strand, within Pf1N1B4_561 at 658.483 kb on + strand, within Pf1N1B4_561 at 658.483 kb on + strand, within Pf1N1B4_561 at 658.519 kb on + strand, within Pf1N1B4_561 at 658.519 kb on + strand, within Pf1N1B4_561 at 658.520 kb on - strand, within Pf1N1B4_561 at 658.527 kb on + strand, within Pf1N1B4_561 at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.712 kb on - strand at 658.712 kb on - strand at 658.713 kb on + strand at 658.713 kb on + strand at 658.713 kb on + strand at 658.713 kb on + strand at 658.713 kb on + strand at 658.714 kb on - strand at 658.714 kb on - strand at 658.714 kb on - strand at 658.776 kb on + strand at 658.777 kb on - strand at 658.777 kb on - strand at 658.786 kb on + strand at 658.786 kb on + strand at 658.786 kb on + strand at 658.787 kb on - strand at 658.787 kb on - strand at 658.787 kb on - strand at 658.787 kb on - strand at 658.787 kb on - strand at 658.799 kb on - strand at 658.799 kb on - strand at 658.799 kb on - strand at 658.799 kb on - strand at 659.165 kb on - strand, within Pf1N1B4_562 at 659.165 kb on - strand, within Pf1N1B4_562 at 659.248 kb on + strand, within Pf1N1B4_562 at 659.248 kb on + strand, within Pf1N1B4_562 at 659.248 kb on + strand, within Pf1N1B4_562 at 659.249 kb on - strand, within Pf1N1B4_562 at 659.249 kb on - strand, within Pf1N1B4_562 at 659.266 kb on + strand at 659.267 kb on - strand, within Pf1N1B4_562 at 659.267 kb on - strand, within Pf1N1B4_562 at 659.418 kb on - strand, within Pf1N1B4_562 at 659.449 kb on + strand, within Pf1N1B4_562 at 659.449 kb on + strand, within Pf1N1B4_562 at 659.449 kb on + strand, within Pf1N1B4_562 at 659.450 kb on - strand, within Pf1N1B4_562 at 659.450 kb on - strand, within Pf1N1B4_562 at 659.450 kb on - strand, within Pf1N1B4_562 at 659.450 kb on - strand, within Pf1N1B4_562 at 659.892 kb on + strand, within Pf1N1B4_563 at 659.892 kb on + strand, within Pf1N1B4_563 at 659.892 kb on + strand, within Pf1N1B4_563 at 659.892 kb on + strand, within Pf1N1B4_563 at 659.893 kb on - strand, within Pf1N1B4_563 at 659.893 kb on - strand, within Pf1N1B4_563 at 659.943 kb on + strand, within Pf1N1B4_563 at 659.943 kb on + strand, within Pf1N1B4_563 at 660.138 kb on + strand, within Pf1N1B4_563 at 660.355 kb on + strand at 660.355 kb on + strand at 660.355 kb on + strand at 660.355 kb on + strand at 660.355 kb on + strand at 660.356 kb on - strand at 660.356 kb on - strand at 660.412 kb on - strand at 660.412 kb on - strand at 660.412 kb on - strand at 660.412 kb on - strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.486 kb on - strand at 660.486 kb on - strand at 660.651 kb on - strand at 660.785 kb on + strand, within Pf1N1B4_564 at 660.785 kb on + strand, within Pf1N1B4_564 at 660.786 kb on - strand, within Pf1N1B4_564
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 657,957 + -2.3 657,958 - +1.8 658,013 + +0.0 658,013 + +1.8 658,013 + +2.3 658,014 - -2.0 658,060 + +0.0 658,060 + -1.0 658,060 + +0.0 658,060 + +2.2 658,060 + +0.6 658,060 + +0.0 658,060 + -2.0 658,061 - +1.3 658,061 - -0.7 658,061 - -1.0 658,061 - +0.0 658,061 - +2.3 658,264 + Pf1N1B4_561 0.35 -0.6 658,265 - Pf1N1B4_561 0.35 +1.0 658,483 + Pf1N1B4_561 0.72 +0.0 658,483 + -1.6 658,483 + +0.0 658,483 + Pf1N1B4_561 0.72 -1.0 658,483 + Pf1N1B4_561 0.72 -1.0 658,483 + Pf1N1B4_561 0.72 +0.0 658,519 + Pf1N1B4_561 0.78 +0.0 658,519 + Pf1N1B4_561 0.78 +2.0 658,520 - Pf1N1B4_561 0.78 -2.8 658,527 + Pf1N1B4_561 0.79 -1.6 658,711 + +2.0 658,711 + +0.6 658,711 + -1.0 658,711 + -1.0 658,711 + +0.0 658,711 + +0.0 658,711 + +0.8 658,711 + +0.0 658,711 + -0.6 658,711 + +0.9 658,711 + -0.7 658,712 - +0.0 658,712 - +1.3 658,713 + -1.0 658,713 + +0.0 658,713 + +1.0 658,713 + +0.6 658,713 + -1.0 658,714 - -1.0 658,714 - -1.6 658,714 - +1.6 658,776 + -1.6 658,777 - -1.3 658,777 - +1.0 658,786 + +0.0 658,786 + +0.0 658,786 + +1.4 658,787 - +0.3 658,787 - +2.3 658,787 - +0.0 658,787 - +2.3 658,787 - -1.0 658,799 - +2.2 658,799 - +0.0 658,799 - -2.0 658,799 - -1.0 659,165 - Pf1N1B4_562 0.36 +1.6 659,165 - Pf1N1B4_562 0.36 +1.3 659,248 + Pf1N1B4_562 0.44 +1.3 659,248 + Pf1N1B4_562 0.44 +0.6 659,248 + Pf1N1B4_562 0.44 -0.6 659,249 - Pf1N1B4_562 0.45 -0.6 659,249 - Pf1N1B4_562 0.45 +1.7 659,266 + +0.0 659,267 - Pf1N1B4_562 0.46 -1.0 659,267 - Pf1N1B4_562 0.46 -1.0 659,418 - Pf1N1B4_562 0.61 +0.4 659,449 + Pf1N1B4_562 0.64 -1.6 659,449 + Pf1N1B4_562 0.64 +0.0 659,449 + Pf1N1B4_562 0.64 +1.0 659,450 - Pf1N1B4_562 0.64 +0.8 659,450 - Pf1N1B4_562 0.64 +0.8 659,450 - Pf1N1B4_562 0.64 +0.6 659,450 - Pf1N1B4_562 0.64 +0.0 659,892 + Pf1N1B4_563 0.15 +0.0 659,892 + Pf1N1B4_563 0.15 +0.0 659,892 + Pf1N1B4_563 0.15 -1.0 659,892 + Pf1N1B4_563 0.15 +0.8 659,893 - Pf1N1B4_563 0.16 -0.6 659,893 - Pf1N1B4_563 0.16 +1.0 659,943 + Pf1N1B4_563 0.25 -1.0 659,943 + Pf1N1B4_563 0.25 -1.0 660,138 + Pf1N1B4_563 0.60 -1.6 660,355 + +2.0 660,355 + +1.3 660,355 + +0.4 660,355 + +1.4 660,355 + +0.4 660,356 - -1.0 660,356 - +0.8 660,412 - +0.0 660,412 - +1.0 660,412 - +1.3 660,412 - +0.6 660,485 + +0.6 660,485 + +0.0 660,485 + -1.0 660,485 + +0.8 660,485 + +1.3 660,485 + -1.0 660,485 + +2.3 660,485 + +1.6 660,485 + +0.8 660,486 - +0.0 660,486 - +0.0 660,651 - -0.4 660,785 + Pf1N1B4_564 0.26 -1.3 660,785 + Pf1N1B4_564 0.26 +0.8 660,786 - Pf1N1B4_564 0.26 +0.0
Or see this region's nucleotide sequence