Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4325 and Pf1N1B4_4326 are separated by 4 nucleotides Pf1N1B4_4326 and Pf1N1B4_4327 overlap by 29 nucleotides
Pf1N1B4_4325 - Agmatine deiminase (EC 3.5.3.12), at 4,785,715 to 4,786,767
_4325
Pf1N1B4_4326 - N-carbamoylputrescine amidase (3.5.1.53), at 4,786,772 to 4,787,680
_4326
Pf1N1B4_4327 - Spermidine/putrescine-binding periplasmic protein, at 4,787,652 to 4,788,785
_4327
Position (kb)
4786
4787
4788 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4786.150 kb on - strand, within Pf1N1B4_4325 at 4786.162 kb on - strand, within Pf1N1B4_4325 at 4786.335 kb on - strand, within Pf1N1B4_4325 at 4786.335 kb on - strand, within Pf1N1B4_4325 at 4786.433 kb on - strand, within Pf1N1B4_4325 at 4786.516 kb on + strand, within Pf1N1B4_4325 at 4786.516 kb on + strand, within Pf1N1B4_4325 at 4786.517 kb on - strand, within Pf1N1B4_4325 at 4786.517 kb on - strand at 4786.579 kb on + strand, within Pf1N1B4_4325 at 4786.917 kb on - strand, within Pf1N1B4_4326 at 4786.917 kb on - strand, within Pf1N1B4_4326 at 4786.917 kb on - strand, within Pf1N1B4_4326 at 4786.980 kb on + strand at 4787.061 kb on - strand, within Pf1N1B4_4326 at 4787.159 kb on + strand, within Pf1N1B4_4326 at 4787.160 kb on - strand, within Pf1N1B4_4326 at 4787.462 kb on + strand, within Pf1N1B4_4326 at 4787.616 kb on - strand at 4787.616 kb on - strand at 4787.779 kb on - strand, within Pf1N1B4_4327 at 4787.794 kb on - strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.043 kb on - strand at 4788.043 kb on - strand at 4788.072 kb on + strand, within Pf1N1B4_4327 at 4788.073 kb on - strand, within Pf1N1B4_4327 at 4788.099 kb on + strand at 4788.201 kb on - strand, within Pf1N1B4_4327 at 4788.201 kb on - strand, within Pf1N1B4_4327 at 4788.249 kb on + strand, within Pf1N1B4_4327 at 4788.250 kb on - strand, within Pf1N1B4_4327 at 4788.320 kb on - strand, within Pf1N1B4_4327 at 4788.327 kb on + strand, within Pf1N1B4_4327 at 4788.332 kb on - strand, within Pf1N1B4_4327 at 4788.543 kb on + strand, within Pf1N1B4_4327 at 4788.544 kb on - strand, within Pf1N1B4_4327 at 4788.672 kb on + strand at 4788.672 kb on + strand at 4788.673 kb on - strand at 4788.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 4,786,150 - Pf1N1B4_4325 0.41 -0.7 4,786,162 - Pf1N1B4_4325 0.42 -0.3 4,786,335 - Pf1N1B4_4325 0.59 -0.3 4,786,335 - Pf1N1B4_4325 0.59 +1.7 4,786,433 - Pf1N1B4_4325 0.68 -0.3 4,786,516 + Pf1N1B4_4325 0.76 -0.3 4,786,516 + Pf1N1B4_4325 0.76 +0.4 4,786,517 - Pf1N1B4_4325 0.76 -0.7 4,786,517 - -0.3 4,786,579 + Pf1N1B4_4325 0.82 +0.1 4,786,917 - Pf1N1B4_4326 0.16 -0.9 4,786,917 - Pf1N1B4_4326 0.16 +1.3 4,786,917 - Pf1N1B4_4326 0.16 -0.6 4,786,980 + +1.3 4,787,061 - Pf1N1B4_4326 0.32 -0.9 4,787,159 + Pf1N1B4_4326 0.43 +0.7 4,787,160 - Pf1N1B4_4326 0.43 +1.0 4,787,462 + Pf1N1B4_4326 0.76 -0.9 4,787,616 - +0.7 4,787,616 - +1.9 4,787,779 - Pf1N1B4_4327 0.11 -0.3 4,787,794 - Pf1N1B4_4327 0.13 +0.1 4,787,850 + Pf1N1B4_4327 0.17 +2.1 4,787,850 + Pf1N1B4_4327 0.17 -1.0 4,787,850 + Pf1N1B4_4327 0.17 +0.3 4,787,850 + Pf1N1B4_4327 0.17 +1.3 4,788,042 + Pf1N1B4_4327 0.34 -1.0 4,788,042 + Pf1N1B4_4327 0.34 -1.9 4,788,042 + Pf1N1B4_4327 0.34 -0.3 4,788,042 + Pf1N1B4_4327 0.34 -1.3 4,788,043 - -0.3 4,788,043 - +1.3 4,788,072 + Pf1N1B4_4327 0.37 -1.1 4,788,073 - Pf1N1B4_4327 0.37 -0.3 4,788,099 + +1.3 4,788,201 - Pf1N1B4_4327 0.48 -1.3 4,788,201 - Pf1N1B4_4327 0.48 -1.3 4,788,249 + Pf1N1B4_4327 0.53 -0.3 4,788,250 - Pf1N1B4_4327 0.53 -0.3 4,788,320 - Pf1N1B4_4327 0.59 -0.3 4,788,327 + Pf1N1B4_4327 0.60 +0.4 4,788,332 - Pf1N1B4_4327 0.60 +0.3 4,788,543 + Pf1N1B4_4327 0.79 -0.3 4,788,544 - Pf1N1B4_4327 0.79 +1.0 4,788,672 + -1.6 4,788,672 + -0.3 4,788,673 - +1.7 4,788,673 - -0.3
Or see this region's nucleotide sequence