Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4324 and Pf1N1B4_4325 are separated by 134 nucleotides Pf1N1B4_4325 and Pf1N1B4_4326 are separated by 4 nucleotides Pf1N1B4_4326 and Pf1N1B4_4327 overlap by 29 nucleotides
Pf1N1B4_4323 - hypothetical protein, at 4,784,583 to 4,784,744
_4323
Pf1N1B4_4324 - Glycine cleavage system transcriptional activator, at 4,784,714 to 4,785,580
_4324
Pf1N1B4_4325 - Agmatine deiminase (EC 3.5.3.12), at 4,785,715 to 4,786,767
_4325
Pf1N1B4_4326 - N-carbamoylputrescine amidase (3.5.1.53), at 4,786,772 to 4,787,680
_4326
Pf1N1B4_4327 - Spermidine/putrescine-binding periplasmic protein, at 4,787,652 to 4,788,785
_4327
Position (kb)
4785
4786
4787 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4784.715 kb on + strand at 4784.715 kb on + strand at 4784.716 kb on - strand at 4784.716 kb on - strand at 4784.716 kb on - strand at 4784.879 kb on + strand at 4784.972 kb on + strand, within Pf1N1B4_4324 at 4785.160 kb on + strand, within Pf1N1B4_4324 at 4785.206 kb on + strand at 4785.206 kb on + strand at 4785.441 kb on + strand, within Pf1N1B4_4324 at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.646 kb on + strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.647 kb on - strand at 4785.708 kb on - strand at 4785.708 kb on - strand at 4786.150 kb on - strand, within Pf1N1B4_4325 at 4786.162 kb on - strand, within Pf1N1B4_4325 at 4786.335 kb on - strand, within Pf1N1B4_4325 at 4786.335 kb on - strand, within Pf1N1B4_4325 at 4786.433 kb on - strand, within Pf1N1B4_4325 at 4786.516 kb on + strand, within Pf1N1B4_4325 at 4786.516 kb on + strand, within Pf1N1B4_4325 at 4786.517 kb on - strand, within Pf1N1B4_4325 at 4786.517 kb on - strand at 4786.579 kb on + strand, within Pf1N1B4_4325 at 4786.917 kb on - strand, within Pf1N1B4_4326 at 4786.917 kb on - strand, within Pf1N1B4_4326 at 4786.917 kb on - strand, within Pf1N1B4_4326 at 4786.980 kb on + strand at 4787.061 kb on - strand, within Pf1N1B4_4326 at 4787.159 kb on + strand, within Pf1N1B4_4326 at 4787.160 kb on - strand, within Pf1N1B4_4326 at 4787.462 kb on + strand, within Pf1N1B4_4326 at 4787.616 kb on - strand at 4787.616 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 4,784,715 + +0.7 4,784,715 + -0.3 4,784,716 - +0.7 4,784,716 - +0.1 4,784,716 - -0.3 4,784,879 + -1.3 4,784,972 + Pf1N1B4_4324 0.30 -0.3 4,785,160 + Pf1N1B4_4324 0.51 +0.7 4,785,206 + -0.3 4,785,206 + +1.0 4,785,441 + Pf1N1B4_4324 0.84 +0.3 4,785,646 + -1.3 4,785,646 + +0.3 4,785,646 + +0.7 4,785,646 + -0.3 4,785,646 + -1.0 4,785,646 + +0.2 4,785,646 + +0.8 4,785,646 + +0.3 4,785,646 + +1.3 4,785,646 + +1.5 4,785,646 + -0.9 4,785,646 + +0.2 4,785,646 + -0.6 4,785,646 + -1.3 4,785,646 + -1.6 4,785,646 + -0.9 4,785,646 + -1.9 4,785,646 + -1.0 4,785,647 - -1.3 4,785,647 - -0.3 4,785,647 - +0.3 4,785,647 - -1.3 4,785,647 - +2.5 4,785,647 - +0.1 4,785,647 - -0.3 4,785,647 - +0.3 4,785,647 - +0.3 4,785,647 - -0.3 4,785,647 - +0.7 4,785,647 - -0.3 4,785,647 - -0.3 4,785,647 - -1.9 4,785,647 - +1.3 4,785,647 - +0.7 4,785,647 - -1.3 4,785,647 - -0.3 4,785,647 - -0.3 4,785,708 - -0.9 4,785,708 - -1.3 4,786,150 - Pf1N1B4_4325 0.41 -0.7 4,786,162 - Pf1N1B4_4325 0.42 -0.3 4,786,335 - Pf1N1B4_4325 0.59 -0.3 4,786,335 - Pf1N1B4_4325 0.59 +1.7 4,786,433 - Pf1N1B4_4325 0.68 -0.3 4,786,516 + Pf1N1B4_4325 0.76 -0.3 4,786,516 + Pf1N1B4_4325 0.76 +0.4 4,786,517 - Pf1N1B4_4325 0.76 -0.7 4,786,517 - -0.3 4,786,579 + Pf1N1B4_4325 0.82 +0.1 4,786,917 - Pf1N1B4_4326 0.16 -0.9 4,786,917 - Pf1N1B4_4326 0.16 +1.3 4,786,917 - Pf1N1B4_4326 0.16 -0.6 4,786,980 + +1.3 4,787,061 - Pf1N1B4_4326 0.32 -0.9 4,787,159 + Pf1N1B4_4326 0.43 +0.7 4,787,160 - Pf1N1B4_4326 0.43 +1.0 4,787,462 + Pf1N1B4_4326 0.76 -0.9 4,787,616 - +0.7 4,787,616 - +1.9
Or see this region's nucleotide sequence