Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3329 and Pf1N1B4_3330 are separated by 112 nucleotides Pf1N1B4_3330 and Pf1N1B4_3331 are separated by 10 nucleotides Pf1N1B4_3331 and Pf1N1B4_3332 are separated by 326 nucleotides
Pf1N1B4_3329 - Putative permease PerM (= YfgO), at 3,717,239 to 3,718,309
_3329
Pf1N1B4_3330 - Thiol peroxidase, Bcp-type (EC 1.11.1.15), at 3,718,422 to 3,718,895
_3330
Pf1N1B4_3331 - Glycine cleavage system transcriptional antiactivator GcvR, at 3,718,906 to 3,719,466
_3331
Pf1N1B4_3332 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 3,719,793 to 3,720,671
_3332
Position (kb)
3718
3719
3720 Strain fitness (log2 ratio)
-1
0
1
2 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand at 3717.920 kb on + strand at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.920 kb on + strand, within Pf1N1B4_3329 at 3717.950 kb on + strand, within Pf1N1B4_3329 at 3717.950 kb on + strand, within Pf1N1B4_3329 at 3717.951 kb on - strand, within Pf1N1B4_3329 at 3718.076 kb on + strand, within Pf1N1B4_3329 at 3718.076 kb on + strand, within Pf1N1B4_3329 at 3718.221 kb on + strand at 3718.307 kb on + strand at 3718.307 kb on + strand at 3718.352 kb on - strand at 3718.375 kb on - strand at 3718.375 kb on - strand at 3718.552 kb on + strand, within Pf1N1B4_3330 at 3718.552 kb on + strand, within Pf1N1B4_3330 at 3718.552 kb on + strand, within Pf1N1B4_3330 at 3718.552 kb on + strand, within Pf1N1B4_3330 at 3718.553 kb on - strand, within Pf1N1B4_3330 at 3718.553 kb on - strand, within Pf1N1B4_3330 at 3718.553 kb on - strand, within Pf1N1B4_3330 at 3718.706 kb on + strand, within Pf1N1B4_3330 at 3718.719 kb on - strand, within Pf1N1B4_3330 at 3718.787 kb on - strand, within Pf1N1B4_3330 at 3718.792 kb on + strand, within Pf1N1B4_3330 at 3718.792 kb on + strand, within Pf1N1B4_3330 at 3718.792 kb on + strand, within Pf1N1B4_3330 at 3718.792 kb on + strand, within Pf1N1B4_3330 at 3718.793 kb on - strand at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.793 kb on - strand at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.793 kb on - strand, within Pf1N1B4_3330 at 3718.907 kb on + strand at 3718.907 kb on + strand at 3718.908 kb on - strand at 3718.908 kb on - strand at 3718.908 kb on - strand at 3719.069 kb on + strand, within Pf1N1B4_3331 at 3719.069 kb on + strand, within Pf1N1B4_3331 at 3719.070 kb on - strand, within Pf1N1B4_3331 at 3719.070 kb on - strand, within Pf1N1B4_3331 at 3719.070 kb on - strand, within Pf1N1B4_3331 at 3719.165 kb on + strand, within Pf1N1B4_3331 at 3719.166 kb on - strand, within Pf1N1B4_3331 at 3719.166 kb on - strand, within Pf1N1B4_3331 at 3719.166 kb on - strand, within Pf1N1B4_3331 at 3719.174 kb on + strand, within Pf1N1B4_3331 at 3719.175 kb on - strand at 3719.496 kb on + strand at 3719.658 kb on + strand at 3719.658 kb on + strand at 3719.659 kb on - strand at 3719.725 kb on - strand at 3719.755 kb on + strand at 3720.111 kb on + strand at 3720.138 kb on + strand, within Pf1N1B4_3332 at 3720.189 kb on + strand at 3720.398 kb on + strand, within Pf1N1B4_3332 at 3720.398 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 3,717,920 + Pf1N1B4_3329 0.64 -0.1 3,717,920 + Pf1N1B4_3329 0.64 -0.1 3,717,920 + +0.9 3,717,920 + -0.1 3,717,920 + Pf1N1B4_3329 0.64 -0.8 3,717,920 + Pf1N1B4_3329 0.64 -0.1 3,717,920 + Pf1N1B4_3329 0.64 -0.9 3,717,920 + Pf1N1B4_3329 0.64 +1.7 3,717,950 + Pf1N1B4_3329 0.66 -1.4 3,717,950 + Pf1N1B4_3329 0.66 -1.1 3,717,951 - Pf1N1B4_3329 0.66 -1.1 3,718,076 + Pf1N1B4_3329 0.78 -0.8 3,718,076 + Pf1N1B4_3329 0.78 +1.9 3,718,221 + -0.1 3,718,307 + -0.1 3,718,307 + -1.6 3,718,352 - -1.6 3,718,375 - -0.1 3,718,375 - -0.1 3,718,552 + Pf1N1B4_3330 0.27 +1.5 3,718,552 + Pf1N1B4_3330 0.27 +0.4 3,718,552 + Pf1N1B4_3330 0.27 -0.1 3,718,552 + Pf1N1B4_3330 0.27 +0.9 3,718,553 - Pf1N1B4_3330 0.28 -0.1 3,718,553 - Pf1N1B4_3330 0.28 +0.3 3,718,553 - Pf1N1B4_3330 0.28 +0.9 3,718,706 + Pf1N1B4_3330 0.60 -1.1 3,718,719 - Pf1N1B4_3330 0.63 +2.7 3,718,787 - Pf1N1B4_3330 0.77 +0.9 3,718,792 + Pf1N1B4_3330 0.78 +2.3 3,718,792 + Pf1N1B4_3330 0.78 -0.1 3,718,792 + Pf1N1B4_3330 0.78 -0.4 3,718,792 + Pf1N1B4_3330 0.78 -1.1 3,718,793 - -0.1 3,718,793 - Pf1N1B4_3330 0.78 -0.3 3,718,793 - Pf1N1B4_3330 0.78 +1.9 3,718,793 - -1.1 3,718,793 - Pf1N1B4_3330 0.78 +0.9 3,718,793 - Pf1N1B4_3330 0.78 +1.3 3,718,793 - Pf1N1B4_3330 0.78 +1.5 3,718,907 + -1.1 3,718,907 + -0.5 3,718,908 - +1.5 3,718,908 - +1.9 3,718,908 - -0.1 3,719,069 + Pf1N1B4_3331 0.29 -1.1 3,719,069 + Pf1N1B4_3331 0.29 -0.1 3,719,070 - Pf1N1B4_3331 0.29 -0.1 3,719,070 - Pf1N1B4_3331 0.29 -0.6 3,719,070 - Pf1N1B4_3331 0.29 +0.9 3,719,165 + Pf1N1B4_3331 0.46 +0.7 3,719,166 - Pf1N1B4_3331 0.46 -1.1 3,719,166 - Pf1N1B4_3331 0.46 -1.1 3,719,166 - Pf1N1B4_3331 0.46 -1.1 3,719,174 + Pf1N1B4_3331 0.48 -0.5 3,719,175 - +0.9 3,719,496 + -0.1 3,719,658 + +1.3 3,719,658 + -1.6 3,719,659 - -1.1 3,719,725 - +2.5 3,719,755 + -0.1 3,720,111 + -0.1 3,720,138 + Pf1N1B4_3332 0.39 -0.1 3,720,189 + -1.6 3,720,398 + Pf1N1B4_3332 0.69 -0.1 3,720,398 + +0.9
Or see this region's nucleotide sequence