Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_280 and Pf1N1B4_281 overlap by 47 nucleotides Pf1N1B4_281 and Pf1N1B4_282 are separated by 14 nucleotides Pf1N1B4_282 and Pf1N1B4_283 are separated by 3 nucleotides Pf1N1B4_283 and Pf1N1B4_284 are separated by 113 nucleotides Pf1N1B4_284 and Pf1N1B4_285 are separated by 96 nucleotides
Pf1N1B4_280 - hypothetical protein, at 338,938 to 339,096
_280
Pf1N1B4_281 - FIG00957049: hypothetical protein, at 339,050 to 339,448
_281
Pf1N1B4_282 - Transcription elongation factor, at 339,463 to 339,945
_282
Pf1N1B4_283 - Cys regulon transcriptional activator CysB, at 339,949 to 340,923
_283
Pf1N1B4_284 - Universal stress protein family, at 341,037 to 341,540
_284
Pf1N1B4_285 - 5'-nucleotidase (EC 3.1.3.5), at 341,637 to 342,542
_285
Position (kb)
339
340
341 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 338.963 kb on + strand, within Pf1N1B4_280 at 338.963 kb on + strand, within Pf1N1B4_280 at 338.963 kb on + strand, within Pf1N1B4_280 at 338.963 kb on + strand at 338.964 kb on - strand, within Pf1N1B4_280 at 338.964 kb on - strand, within Pf1N1B4_280 at 338.969 kb on - strand, within Pf1N1B4_280 at 338.996 kb on + strand, within Pf1N1B4_280 at 338.997 kb on - strand, within Pf1N1B4_280 at 338.997 kb on - strand, within Pf1N1B4_280 at 339.049 kb on - strand, within Pf1N1B4_280 at 339.094 kb on + strand at 339.094 kb on + strand at 339.095 kb on - strand at 339.095 kb on - strand at 339.118 kb on + strand, within Pf1N1B4_281 at 339.119 kb on - strand, within Pf1N1B4_281 at 339.288 kb on - strand, within Pf1N1B4_281 at 339.288 kb on - strand, within Pf1N1B4_281 at 339.288 kb on - strand, within Pf1N1B4_281 at 339.288 kb on - strand at 339.347 kb on - strand, within Pf1N1B4_281 at 339.347 kb on - strand, within Pf1N1B4_281 at 339.589 kb on + strand, within Pf1N1B4_282 at 339.589 kb on + strand at 339.589 kb on + strand, within Pf1N1B4_282 at 339.590 kb on - strand, within Pf1N1B4_282 at 339.590 kb on - strand, within Pf1N1B4_282 at 339.617 kb on - strand, within Pf1N1B4_282 at 339.617 kb on - strand, within Pf1N1B4_282 at 339.617 kb on - strand, within Pf1N1B4_282 at 339.697 kb on + strand, within Pf1N1B4_282 at 339.697 kb on + strand, within Pf1N1B4_282 at 339.697 kb on + strand, within Pf1N1B4_282 at 339.943 kb on + strand at 339.943 kb on + strand at 340.944 kb on - strand at 340.944 kb on - strand at 340.944 kb on - strand at 340.969 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.977 kb on - strand at 340.977 kb on - strand at 340.977 kb on - strand at 340.979 kb on - strand at 341.003 kb on - strand at 341.039 kb on - strand at 341.039 kb on - strand at 341.081 kb on - strand at 341.081 kb on - strand at 341.081 kb on - strand at 341.081 kb on - strand at 341.167 kb on - strand, within Pf1N1B4_284 at 341.337 kb on - strand, within Pf1N1B4_284 at 341.337 kb on - strand, within Pf1N1B4_284 at 341.383 kb on + strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.500 kb on + strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.583 kb on + strand at 341.584 kb on - strand at 341.584 kb on - strand at 341.639 kb on - strand at 341.654 kb on + strand at 341.733 kb on + strand, within Pf1N1B4_285 at 341.733 kb on + strand, within Pf1N1B4_285 at 341.733 kb on + strand, within Pf1N1B4_285 at 341.734 kb on - strand, within Pf1N1B4_285
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 338,963 + Pf1N1B4_280 0.16 +1.9 338,963 + Pf1N1B4_280 0.16 +1.3 338,963 + Pf1N1B4_280 0.16 -0.1 338,963 + -0.1 338,964 - Pf1N1B4_280 0.16 +0.5 338,964 - Pf1N1B4_280 0.16 +1.1 338,969 - Pf1N1B4_280 0.19 -0.5 338,996 + Pf1N1B4_280 0.36 +0.9 338,997 - Pf1N1B4_280 0.37 +0.3 338,997 - Pf1N1B4_280 0.37 +1.6 339,049 - Pf1N1B4_280 0.70 +1.5 339,094 + -0.1 339,094 + +1.5 339,095 - +0.9 339,095 - +1.5 339,118 + Pf1N1B4_281 0.17 +0.9 339,119 - Pf1N1B4_281 0.17 +0.6 339,288 - Pf1N1B4_281 0.60 -0.5 339,288 - Pf1N1B4_281 0.60 -1.2 339,288 - Pf1N1B4_281 0.60 -0.3 339,288 - -0.1 339,347 - Pf1N1B4_281 0.74 -0.4 339,347 - Pf1N1B4_281 0.74 +2.5 339,589 + Pf1N1B4_282 0.26 +1.3 339,589 + -0.1 339,589 + Pf1N1B4_282 0.26 -1.1 339,590 - Pf1N1B4_282 0.26 +0.5 339,590 - Pf1N1B4_282 0.26 -0.1 339,617 - Pf1N1B4_282 0.32 +1.5 339,617 - Pf1N1B4_282 0.32 +0.2 339,617 - Pf1N1B4_282 0.32 +1.5 339,697 + Pf1N1B4_282 0.48 -1.4 339,697 + Pf1N1B4_282 0.48 -0.8 339,697 + Pf1N1B4_282 0.48 +1.2 339,943 + +1.2 339,943 + -0.1 340,944 - -1.1 340,944 - +0.4 340,944 - -0.3 340,969 - -0.7 340,971 - -0.3 340,971 - -0.4 340,971 - -1.4 340,971 - -0.7 340,971 - +0.7 340,971 - -0.1 340,971 - -1.7 340,977 - -1.1 340,977 - -1.1 340,977 - -0.1 340,979 - -0.1 341,003 - +0.9 341,039 - +1.3 341,039 - +0.5 341,081 - -0.9 341,081 - -0.1 341,081 - +0.9 341,081 - -0.6 341,167 - Pf1N1B4_284 0.26 +0.9 341,337 - Pf1N1B4_284 0.60 +0.0 341,337 - Pf1N1B4_284 0.60 +1.5 341,383 + Pf1N1B4_284 0.69 -1.7 341,384 - Pf1N1B4_284 0.69 -0.6 341,384 - Pf1N1B4_284 0.69 -0.1 341,384 - Pf1N1B4_284 0.69 +0.6 341,384 - Pf1N1B4_284 0.69 +1.1 341,384 - Pf1N1B4_284 0.69 +0.1 341,384 - Pf1N1B4_284 0.69 -1.8 341,384 - Pf1N1B4_284 0.69 +1.4 341,440 + Pf1N1B4_284 0.80 +0.9 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + +0.9 341,440 + Pf1N1B4_284 0.80 -1.4 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + Pf1N1B4_284 0.80 +0.2 341,440 + Pf1N1B4_284 0.80 +0.5 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + Pf1N1B4_284 0.80 +1.2 341,440 + Pf1N1B4_284 0.80 +0.9 341,440 + Pf1N1B4_284 0.80 -1.1 341,440 + Pf1N1B4_284 0.80 +1.5 341,440 + Pf1N1B4_284 0.80 -0.5 341,440 + Pf1N1B4_284 0.80 -0.5 341,440 + Pf1N1B4_284 0.80 +0.4 341,440 + -0.1 341,440 + Pf1N1B4_284 0.80 +0.9 341,440 + Pf1N1B4_284 0.80 -1.1 341,440 + Pf1N1B4_284 0.80 -2.9 341,441 - Pf1N1B4_284 0.80 +0.9 341,441 - Pf1N1B4_284 0.80 -1.1 341,441 - Pf1N1B4_284 0.80 +0.6 341,441 - Pf1N1B4_284 0.80 -0.7 341,441 - Pf1N1B4_284 0.80 -0.7 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 +0.6 341,441 - Pf1N1B4_284 0.80 +2.2 341,441 - Pf1N1B4_284 0.80 +0.5 341,441 - Pf1N1B4_284 0.80 -0.3 341,441 - Pf1N1B4_284 0.80 -1.4 341,441 - Pf1N1B4_284 0.80 +0.3 341,441 - Pf1N1B4_284 0.80 -0.4 341,441 - Pf1N1B4_284 0.80 -0.4 341,441 - Pf1N1B4_284 0.80 -2.1 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 +0.6 341,441 - Pf1N1B4_284 0.80 -0.2 341,441 - Pf1N1B4_284 0.80 -1.1 341,441 - Pf1N1B4_284 0.80 -0.6 341,441 - Pf1N1B4_284 0.80 +0.6 341,441 - Pf1N1B4_284 0.80 +1.1 341,441 - -1.1 341,441 - Pf1N1B4_284 0.80 +1.1 341,441 - Pf1N1B4_284 0.80 +0.9 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 +0.9 341,441 - Pf1N1B4_284 0.80 +0.5 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -1.4 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - -0.1 341,441 - Pf1N1B4_284 0.80 +0.4 341,441 - Pf1N1B4_284 0.80 -0.7 341,441 - Pf1N1B4_284 0.80 -0.4 341,441 - Pf1N1B4_284 0.80 +0.5 341,441 - Pf1N1B4_284 0.80 -0.8 341,441 - Pf1N1B4_284 0.80 +1.5 341,441 - Pf1N1B4_284 0.80 +0.3 341,441 - Pf1N1B4_284 0.80 +2.7 341,441 - Pf1N1B4_284 0.80 -0.7 341,441 - Pf1N1B4_284 0.80 +1.5 341,441 - Pf1N1B4_284 0.80 +0.9 341,441 - Pf1N1B4_284 0.80 +0.2 341,441 - Pf1N1B4_284 0.80 +0.6 341,500 + +2.1 341,501 - +0.5 341,501 - -0.5 341,501 - +0.7 341,501 - -1.1 341,501 - +0.5 341,501 - +0.2 341,501 - +0.9 341,501 - -0.1 341,501 - -0.1 341,521 + +0.9 341,521 + +0.3 341,521 + -0.4 341,521 + +0.8 341,521 + +1.2 341,521 + -1.1 341,521 + +0.4 341,521 + -1.7 341,522 - +0.6 341,522 - +0.2 341,522 - +1.2 341,522 - +0.6 341,522 - +0.3 341,522 - +0.9 341,522 - -0.1 341,522 - +0.2 341,522 - -1.5 341,522 - -0.5 341,522 - +0.8 341,522 - +0.8 341,522 - +0.4 341,522 - -0.1 341,522 - -1.1 341,522 - -0.1 341,522 - -0.1 341,522 - +0.0 341,522 - -0.1 341,522 - +1.4 341,522 - +1.5 341,522 - -0.8 341,583 + +0.7 341,584 - -0.1 341,584 - -0.7 341,639 - +1.3 341,654 + -0.1 341,733 + Pf1N1B4_285 0.11 -1.1 341,733 + Pf1N1B4_285 0.11 -1.1 341,733 + Pf1N1B4_285 0.11 -0.4 341,734 - Pf1N1B4_285 0.11 -0.5
Or see this region's nucleotide sequence