Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2351 and Pf1N1B4_2352 are separated by 151 nucleotides Pf1N1B4_2352 and Pf1N1B4_2353 are separated by 233 nucleotides Pf1N1B4_2353 and Pf1N1B4_2354 are separated by 57 nucleotides
Pf1N1B4_2351 - Ferric siderophore transport system, periplasmic binding protein TonB, at 2,665,244 to 2,666,098
_2351
Pf1N1B4_2352 - Glutathione synthetase (EC 6.3.2.3), at 2,666,250 to 2,667,221
_2352
Pf1N1B4_2353 - twitching motility protein PilG, at 2,667,455 to 2,667,859
_2353
Pf1N1B4_2354 - twitching motility protein PilH, at 2,667,917 to 2,668,282
_2354
Position (kb)
2666
2667
2668 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2665.250 kb on - strand at 2665.250 kb on - strand at 2666.159 kb on + strand at 2666.160 kb on - strand at 2666.160 kb on - strand at 2666.160 kb on - strand at 2666.214 kb on + strand at 2666.215 kb on - strand at 2666.215 kb on - strand at 2666.215 kb on - strand at 2666.215 kb on - strand at 2666.215 kb on - strand at 2666.237 kb on + strand at 2666.238 kb on - strand at 2666.238 kb on - strand at 2666.292 kb on + strand at 2666.293 kb on - strand at 2666.397 kb on + strand, within Pf1N1B4_2352 at 2666.397 kb on + strand, within Pf1N1B4_2352 at 2666.398 kb on - strand, within Pf1N1B4_2352 at 2666.621 kb on - strand, within Pf1N1B4_2352 at 2666.920 kb on + strand, within Pf1N1B4_2352 at 2666.920 kb on + strand, within Pf1N1B4_2352 at 2666.920 kb on + strand, within Pf1N1B4_2352 at 2666.921 kb on - strand, within Pf1N1B4_2352 at 2666.921 kb on - strand, within Pf1N1B4_2352 at 2666.921 kb on - strand, within Pf1N1B4_2352 at 2666.921 kb on - strand, within Pf1N1B4_2352 at 2666.929 kb on + strand, within Pf1N1B4_2352 at 2666.930 kb on - strand, within Pf1N1B4_2352 at 2667.088 kb on + strand, within Pf1N1B4_2352 at 2667.089 kb on - strand, within Pf1N1B4_2352 at 2667.108 kb on - strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.162 kb on - strand at 2667.170 kb on + strand at 2667.170 kb on + strand at 2667.171 kb on - strand at 2667.172 kb on + strand at 2667.172 kb on + strand at 2667.172 kb on + strand at 2667.172 kb on + strand at 2667.189 kb on - strand at 2667.328 kb on - strand at 2667.379 kb on + strand at 2667.379 kb on + strand at 2667.379 kb on + strand at 2667.380 kb on - strand at 2667.380 kb on - strand at 2667.380 kb on - strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.446 kb on - strand at 2667.446 kb on - strand at 2667.670 kb on - strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.829 kb on - strand at 2667.857 kb on + strand at 2667.858 kb on - strand at 2667.925 kb on + strand at 2667.925 kb on + strand at 2667.925 kb on + strand at 2667.925 kb on + strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2668.160 kb on - strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 2,665,250 - +1.2 2,665,250 - +2.2 2,666,159 + +0.2 2,666,160 - +1.2 2,666,160 - -1.4 2,666,160 - +0.5 2,666,214 + -0.1 2,666,215 - +0.9 2,666,215 - -0.8 2,666,215 - +0.2 2,666,215 - +1.2 2,666,215 - -1.0 2,666,237 + -0.4 2,666,238 - -1.8 2,666,238 - -0.4 2,666,292 + -1.4 2,666,293 - +2.5 2,666,397 + Pf1N1B4_2352 0.15 -1.4 2,666,397 + Pf1N1B4_2352 0.15 -1.1 2,666,398 - Pf1N1B4_2352 0.15 +0.8 2,666,621 - Pf1N1B4_2352 0.38 +0.2 2,666,920 + Pf1N1B4_2352 0.69 +0.2 2,666,920 + Pf1N1B4_2352 0.69 -0.8 2,666,920 + Pf1N1B4_2352 0.69 +1.2 2,666,921 - Pf1N1B4_2352 0.69 -1.6 2,666,921 - Pf1N1B4_2352 0.69 +1.2 2,666,921 - Pf1N1B4_2352 0.69 -0.8 2,666,921 - Pf1N1B4_2352 0.69 +0.2 2,666,929 + Pf1N1B4_2352 0.70 +0.2 2,666,930 - Pf1N1B4_2352 0.70 +1.2 2,667,088 + Pf1N1B4_2352 0.86 -0.4 2,667,089 - Pf1N1B4_2352 0.86 +0.2 2,667,108 - Pf1N1B4_2352 0.88 -0.8 2,667,109 + Pf1N1B4_2352 0.88 +1.2 2,667,109 + Pf1N1B4_2352 0.88 -2.1 2,667,109 + Pf1N1B4_2352 0.88 -0.8 2,667,109 + Pf1N1B4_2352 0.88 +0.2 2,667,109 + Pf1N1B4_2352 0.88 +0.2 2,667,109 + Pf1N1B4_2352 0.88 -3.0 2,667,109 + +0.2 2,667,109 + Pf1N1B4_2352 0.88 -0.8 2,667,109 + Pf1N1B4_2352 0.88 -0.8 2,667,109 + Pf1N1B4_2352 0.88 -0.8 2,667,109 + Pf1N1B4_2352 0.88 -0.8 2,667,109 + Pf1N1B4_2352 0.88 -1.4 2,667,110 - Pf1N1B4_2352 0.88 +0.2 2,667,110 - +0.2 2,667,110 - Pf1N1B4_2352 0.88 -1.1 2,667,110 - Pf1N1B4_2352 0.88 -2.1 2,667,110 - Pf1N1B4_2352 0.88 +0.2 2,667,110 - Pf1N1B4_2352 0.88 -2.1 2,667,110 - Pf1N1B4_2352 0.88 -0.8 2,667,110 - Pf1N1B4_2352 0.88 -1.4 2,667,110 - Pf1N1B4_2352 0.88 +0.2 2,667,110 - Pf1N1B4_2352 0.88 -1.8 2,667,162 - +0.2 2,667,170 + -0.8 2,667,170 + -2.6 2,667,171 - -0.8 2,667,172 + +0.2 2,667,172 + +1.8 2,667,172 + -1.6 2,667,172 + +0.2 2,667,189 - -1.4 2,667,328 - +0.2 2,667,379 + +0.3 2,667,379 + +1.8 2,667,379 + -0.4 2,667,380 - -0.6 2,667,380 - +2.0 2,667,380 - +0.2 2,667,383 + +0.2 2,667,383 + +0.2 2,667,383 + +1.2 2,667,383 + -0.0 2,667,383 + +0.2 2,667,383 + +2.5 2,667,384 - -0.6 2,667,384 - +0.2 2,667,384 - +2.2 2,667,384 - +0.2 2,667,384 - +0.2 2,667,384 - +0.9 2,667,384 - +0.5 2,667,384 - +0.2 2,667,384 - -0.4 2,667,384 - +0.8 2,667,384 - +0.2 2,667,446 - +0.2 2,667,446 - +0.6 2,667,670 - Pf1N1B4_2353 0.53 -2.1 2,667,709 + Pf1N1B4_2353 0.63 -0.8 2,667,709 + Pf1N1B4_2353 0.63 +1.2 2,667,709 + Pf1N1B4_2353 0.63 +1.5 2,667,709 + Pf1N1B4_2353 0.63 -1.8 2,667,709 + Pf1N1B4_2353 0.63 +1.2 2,667,709 + Pf1N1B4_2353 0.63 +1.8 2,667,709 + Pf1N1B4_2353 0.63 -0.8 2,667,709 + Pf1N1B4_2353 0.63 +0.2 2,667,710 - Pf1N1B4_2353 0.63 +0.2 2,667,710 - Pf1N1B4_2353 0.63 -0.2 2,667,710 - Pf1N1B4_2353 0.63 +0.2 2,667,710 - Pf1N1B4_2353 0.63 -0.8 2,667,710 - Pf1N1B4_2353 0.63 -0.6 2,667,829 - +0.7 2,667,857 + -0.8 2,667,858 - +0.2 2,667,925 + +1.2 2,667,925 + +0.8 2,667,925 + -1.4 2,667,925 + +1.3 2,667,926 - +0.8 2,667,926 - -1.4 2,667,926 - +1.4 2,667,926 - +0.2 2,667,926 - -0.6 2,667,926 - -2.1 2,667,926 - -1.4 2,667,926 - -0.8 2,667,926 - +0.4 2,667,926 - +0.2 2,667,926 - +0.2 2,667,926 - -0.4 2,667,959 + Pf1N1B4_2354 0.11 -0.4 2,667,959 + Pf1N1B4_2354 0.11 +1.8 2,667,959 + Pf1N1B4_2354 0.11 -0.2 2,667,959 + Pf1N1B4_2354 0.11 +0.2 2,667,959 + Pf1N1B4_2354 0.11 +0.9 2,667,960 - Pf1N1B4_2354 0.12 +1.8 2,667,960 - Pf1N1B4_2354 0.12 +1.2 2,667,960 - Pf1N1B4_2354 0.12 +0.2 2,668,160 - Pf1N1B4_2354 0.66 -0.8 2,668,217 + Pf1N1B4_2354 0.82 -0.1 2,668,217 + Pf1N1B4_2354 0.82 +2.5 2,668,217 + Pf1N1B4_2354 0.82 +0.2 2,668,217 + Pf1N1B4_2354 0.82 +0.2 2,668,218 - Pf1N1B4_2354 0.82 +0.4 2,668,218 - Pf1N1B4_2354 0.82 +0.5 2,668,218 - Pf1N1B4_2354 0.82 +0.8 2,668,218 - Pf1N1B4_2354 0.82 +0.2 2,668,218 - Pf1N1B4_2354 0.82 -2.1
Or see this region's nucleotide sequence