Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_19

Experiment: Uridine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_17 and Pf1N1B4_18 are separated by 103 nucleotidesPf1N1B4_18 and Pf1N1B4_19 are separated by 120 nucleotidesPf1N1B4_19 and Pf1N1B4_20 are separated by 166 nucleotidesPf1N1B4_20 and Pf1N1B4_21 are separated by 48 nucleotides Pf1N1B4_17 - Sensory box/GGDEF family protein, at 18,598 to 21,855 _17 Pf1N1B4_18 - 16 kDa heat shock protein A, at 21,959 to 22,405 _18 Pf1N1B4_19 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 22,526 to 23,485 _19 Pf1N1B4_20 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 23,652 to 24,101 _20 Pf1N1B4_21 - Probable hydrolase, at 24,150 to 24,983 _21 Position (kb) 22 23 24Strain fitness (log2 ratio) -2 -1 0 1 2 3at 21.598 kb on + strandat 21.598 kb on + strandat 21.794 kb on - strandat 21.794 kb on - strandat 21.802 kb on + strandat 21.802 kb on + strandat 21.802 kb on + strandat 21.802 kb on + strandat 21.853 kb on + strandat 21.885 kb on + strandat 21.893 kb on + strandat 21.893 kb on + strandat 22.141 kb on - strand, within Pf1N1B4_18at 22.141 kb on - strand, within Pf1N1B4_18at 22.141 kb on - strand, within Pf1N1B4_18at 22.146 kb on + strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.147 kb on - strand, within Pf1N1B4_18at 22.290 kb on + strand, within Pf1N1B4_18at 22.291 kb on - strand, within Pf1N1B4_18at 22.299 kb on + strand, within Pf1N1B4_18at 22.300 kb on - strand, within Pf1N1B4_18at 22.300 kb on - strand, within Pf1N1B4_18at 22.400 kb on - strandat 22.400 kb on - strandat 22.425 kb on + strandat 22.472 kb on - strandat 22.483 kb on - strandat 22.483 kb on - strandat 22.483 kb on - strandat 22.556 kb on + strandat 22.623 kb on + strand, within Pf1N1B4_19at 22.959 kb on + strand, within Pf1N1B4_19at 22.959 kb on + strand, within Pf1N1B4_19at 23.349 kb on + strand, within Pf1N1B4_19at 23.353 kb on - strand, within Pf1N1B4_19at 23.353 kb on - strand, within Pf1N1B4_19at 23.362 kb on + strand, within Pf1N1B4_19at 23.363 kb on - strand, within Pf1N1B4_19at 23.363 kb on - strand, within Pf1N1B4_19at 23.386 kb on + strand, within Pf1N1B4_19at 23.594 kb on - strandat 23.692 kb on + strandat 23.882 kb on - strand, within Pf1N1B4_20at 23.914 kb on - strand, within Pf1N1B4_20at 24.011 kb on - strand, within Pf1N1B4_20at 24.121 kb on + strandat 24.122 kb on - strandat 24.122 kb on - strandat 24.122 kb on - strandat 24.252 kb on + strand, within Pf1N1B4_21at 24.253 kb on - strand, within Pf1N1B4_21at 24.365 kb on - strand, within Pf1N1B4_21at 24.423 kb on - strand, within Pf1N1B4_21at 24.423 kb on - strandat 24.458 kb on - strand, within Pf1N1B4_21at 24.458 kb on - strand, within Pf1N1B4_21at 24.458 kb on - strand, within Pf1N1B4_21at 24.458 kb on - strand, within Pf1N1B4_21at 24.478 kb on - strand, within Pf1N1B4_21

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Per-strain Table

Position Strand Gene LocusTag Fraction Uridine (N)
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21,598 + -1.0
21,598 + +0.0
21,794 - +0.8
21,794 - -0.2
21,802 + -1.8
21,802 + -1.2
21,802 + -0.2
21,802 + +0.8
21,853 + +1.4
21,885 + +0.8
21,893 + -1.2
21,893 + +2.8
22,141 - Pf1N1B4_18 0.41 -1.8
22,141 - Pf1N1B4_18 0.41 +0.8
22,141 - Pf1N1B4_18 0.41 +1.8
22,146 + Pf1N1B4_18 0.42 +0.6
22,147 - Pf1N1B4_18 0.42 -0.2
22,147 - Pf1N1B4_18 0.42 -0.2
22,147 - Pf1N1B4_18 0.42 +0.8
22,147 - Pf1N1B4_18 0.42 +2.1
22,147 - Pf1N1B4_18 0.42 +0.4
22,147 - Pf1N1B4_18 0.42 -1.2
22,147 - Pf1N1B4_18 0.42 -0.8
22,147 - Pf1N1B4_18 0.42 +1.4
22,147 - Pf1N1B4_18 0.42 +0.8
22,147 - Pf1N1B4_18 0.42 -0.8
22,147 - Pf1N1B4_18 0.42 +0.7
22,290 + Pf1N1B4_18 0.74 -0.2
22,291 - Pf1N1B4_18 0.74 +0.8
22,299 + Pf1N1B4_18 0.76 +0.8
22,300 - Pf1N1B4_18 0.76 -0.2
22,300 - Pf1N1B4_18 0.76 +0.8
22,400 - -0.8
22,400 - +1.4
22,425 + +0.2
22,472 - -1.2
22,483 - -0.2
22,483 - +0.8
22,483 - +2.8
22,556 + -0.2
22,623 + Pf1N1B4_19 0.10 -1.2
22,959 + Pf1N1B4_19 0.45 +0.8
22,959 + Pf1N1B4_19 0.45 -1.2
23,349 + Pf1N1B4_19 0.86 -0.4
23,353 - Pf1N1B4_19 0.86 -2.2
23,353 - Pf1N1B4_19 0.86 -0.8
23,362 + Pf1N1B4_19 0.87 -0.6
23,363 - Pf1N1B4_19 0.87 -0.8
23,363 - Pf1N1B4_19 0.87 +0.6
23,386 + Pf1N1B4_19 0.90 -1.2
23,594 - -1.8
23,692 + +0.4
23,882 - Pf1N1B4_20 0.51 +0.8
23,914 - Pf1N1B4_20 0.58 -0.2
24,011 - Pf1N1B4_20 0.80 -0.2
24,121 + -0.2
24,122 - -0.2
24,122 - +0.8
24,122 - -0.2
24,252 + Pf1N1B4_21 0.12 -1.5
24,253 - Pf1N1B4_21 0.12 -1.8
24,365 - Pf1N1B4_21 0.26 -1.8
24,423 - Pf1N1B4_21 0.33 +0.4
24,423 - +1.8
24,458 - Pf1N1B4_21 0.37 -1.2
24,458 - Pf1N1B4_21 0.37 +1.4
24,458 - Pf1N1B4_21 0.37 +1.4
24,458 - Pf1N1B4_21 0.37 -0.9
24,478 - Pf1N1B4_21 0.39 -0.2

Or see this region's nucleotide sequence