Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_15 and Pf1N1B4_16 are separated by 158 nucleotides Pf1N1B4_16 and Pf1N1B4_17 are separated by 105 nucleotides
Pf1N1B4_15 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 16,025 to 17,443
_15
Pf1N1B4_16 - Cys regulon transcriptional activator CysB, at 17,602 to 18,492
_16
Pf1N1B4_17 - Sensory box/GGDEF family protein, at 18,598 to 21,855
_17
Position (kb) 17
18
19 Strain fitness (log2 ratio) -2
-1
0
1 at 16.704 kb on + strand, within Pf1N1B4_15 at 16.704 kb on + strand, within Pf1N1B4_15 at 16.704 kb on + strand, within Pf1N1B4_15 at 16.774 kb on + strand, within Pf1N1B4_15 at 16.775 kb on - strand, within Pf1N1B4_15 at 17.165 kb on - strand, within Pf1N1B4_15 at 17.364 kb on + strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.471 kb on - strand at 17.823 kb on - strand, within Pf1N1B4_16 at 17.927 kb on + strand, within Pf1N1B4_16 at 17.931 kb on - strand, within Pf1N1B4_16 at 18.271 kb on + strand, within Pf1N1B4_16 at 18.272 kb on - strand at 18.392 kb on + strand, within Pf1N1B4_16 at 18.392 kb on + strand, within Pf1N1B4_16 at 18.392 kb on + strand, within Pf1N1B4_16 at 18.398 kb on - strand at 18.398 kb on - strand, within Pf1N1B4_16 at 18.521 kb on + strand at 18.522 kb on - strand at 18.524 kb on - strand at 18.597 kb on + strand at 18.598 kb on - strand at 18.598 kb on - strand at 18.709 kb on + strand at 18.805 kb on + strand at 18.805 kb on + strand at 19.136 kb on - strand, within Pf1N1B4_17 at 19.136 kb on - strand, within Pf1N1B4_17 at 19.355 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 16,704 + Pf1N1B4_15 0.48 -1.2 16,704 + Pf1N1B4_15 0.48 -2.2 16,704 + Pf1N1B4_15 0.48 -1.2 16,774 + Pf1N1B4_15 0.53 -1.2 16,775 - Pf1N1B4_15 0.53 -0.2 17,165 - Pf1N1B4_15 0.80 -2.5 17,364 + -0.2 17,365 - -0.2 17,365 - -2.5 17,365 - -1.2 17,365 - -1.8 17,365 - -2.2 17,471 - -0.2 17,823 - Pf1N1B4_16 0.25 +1.4 17,927 + Pf1N1B4_16 0.36 -0.8 17,931 - Pf1N1B4_16 0.37 +0.8 18,271 + Pf1N1B4_16 0.75 +0.8 18,272 - +0.4 18,392 + Pf1N1B4_16 0.89 -1.2 18,392 + Pf1N1B4_16 0.89 -1.5 18,392 + Pf1N1B4_16 0.89 +0.4 18,398 - -0.2 18,398 - Pf1N1B4_16 0.89 +0.6 18,521 + -0.2 18,522 - -0.2 18,524 - +1.0 18,597 + -0.2 18,598 - -0.2 18,598 - +1.4 18,709 + -1.2 18,805 + +1.6 18,805 + +0.8 19,136 - Pf1N1B4_17 0.17 -0.2 19,136 - Pf1N1B4_17 0.17 -0.7 19,355 - -0.2
Or see this region's nucleotide sequence