Experiment: ALP with copper (II) chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2480 and Dsui_2481 overlap by 4 nucleotides Dsui_2481 and Dsui_2482 are separated by 71 nucleotides Dsui_2482 and Dsui_2483 overlap by 11 nucleotides
Dsui_2480: Dsui_2480 - nitrous oxidase accessory protein, at 2,630,518 to 2,631,753
_2480
Dsui_2481: Dsui_2481 - DNA polymerase III, epsilon subunit, at 2,631,750 to 2,632,469
_2481
Dsui_2482: Dsui_2482 - ribonuclease HI, at 2,632,541 to 2,632,993
_2482
Dsui_2483: Dsui_2483 - methylase involved in ubiquinone/menaquinone biosynthesis, at 2,632,983 to 2,633,711
_2483
Position (kb)
2631
2632
2633 Strain fitness (log2 ratio)
-1
0
1 at 2630.792 kb on + strand, within Dsui_2480 at 2630.799 kb on - strand, within Dsui_2480 at 2630.884 kb on + strand at 2631.108 kb on - strand, within Dsui_2480 at 2631.293 kb on - strand, within Dsui_2480 at 2631.381 kb on + strand at 2631.415 kb on + strand, within Dsui_2480 at 2631.428 kb on - strand, within Dsui_2480 at 2631.493 kb on - strand, within Dsui_2480 at 2631.663 kb on + strand at 2631.734 kb on - strand at 2632.499 kb on - strand at 2632.524 kb on - strand at 2632.524 kb on - strand at 2632.545 kb on - strand at 2632.975 kb on - strand at 2632.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with copper (II) chloride 0.6 mM remove 2,630,792 + Dsui_2480 0.22 -1.0 2,630,799 - Dsui_2480 0.23 +0.1 2,630,884 + +0.6 2,631,108 - Dsui_2480 0.48 -0.1 2,631,293 - Dsui_2480 0.63 -1.4 2,631,381 + +0.6 2,631,415 + Dsui_2480 0.73 -0.4 2,631,428 - Dsui_2480 0.74 -1.2 2,631,493 - Dsui_2480 0.79 -1.4 2,631,663 + +0.6 2,631,734 - -1.2 2,632,499 - -1.2 2,632,524 - -1.2 2,632,524 - -1.3 2,632,545 - -1.3 2,632,975 - -1.4 2,632,998 - -0.9
Or see this region's nucleotide sequence