Experiment: ALP with copper (II) chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1366 and Dsui_1367 are separated by 120 nucleotides Dsui_1367 and Dsui_1368 are separated by 8 nucleotides Dsui_1368 and Dsui_1369 are separated by 50 nucleotides Dsui_1369 and Dsui_1370 are separated by 141 nucleotides
Dsui_1366: Dsui_1366 - glutaminyl-tRNA synthetase, at 1,419,730 to 1,421,511
_1366
Dsui_1367: Dsui_1367 - crcB protein, at 1,421,632 to 1,422,021
_1367
Dsui_1368: Dsui_1368 - hypothetical protein, at 1,422,030 to 1,422,866
_1368
Dsui_1369: Dsui_1369 - hypothetical protein, at 1,422,917 to 1,423,207
_1369
Dsui_1370: Dsui_1370 - putative permease, DMT superfamily, at 1,423,349 to 1,424,251
_1370
Position (kb)
1422
1423 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1421.588 kb on - strand at 1421.609 kb on + strand at 1421.609 kb on + strand at 1421.609 kb on + strand at 1421.609 kb on + strand at 1421.609 kb on + strand at 1421.617 kb on - strand at 1421.617 kb on - strand at 1421.624 kb on + strand at 1421.624 kb on - strand at 1421.632 kb on - strand at 1421.709 kb on + strand, within Dsui_1367 at 1421.721 kb on + strand, within Dsui_1367 at 1421.724 kb on - strand, within Dsui_1367 at 1421.724 kb on - strand at 1421.780 kb on + strand, within Dsui_1367 at 1421.814 kb on + strand, within Dsui_1367 at 1421.815 kb on - strand at 1421.826 kb on + strand, within Dsui_1367 at 1421.947 kb on - strand, within Dsui_1367 at 1422.015 kb on + strand at 1422.092 kb on - strand at 1422.103 kb on - strand at 1422.110 kb on - strand at 1422.152 kb on + strand, within Dsui_1368 at 1422.171 kb on - strand at 1422.175 kb on - strand at 1422.191 kb on - strand, within Dsui_1368 at 1422.217 kb on - strand, within Dsui_1368 at 1422.225 kb on - strand, within Dsui_1368 at 1422.239 kb on + strand, within Dsui_1368 at 1422.247 kb on - strand, within Dsui_1368 at 1422.350 kb on - strand at 1422.453 kb on + strand, within Dsui_1368 at 1422.466 kb on - strand, within Dsui_1368 at 1422.569 kb on + strand at 1422.569 kb on + strand, within Dsui_1368 at 1422.667 kb on - strand at 1422.713 kb on - strand at 1422.715 kb on + strand at 1422.743 kb on + strand, within Dsui_1368 at 1422.743 kb on + strand, within Dsui_1368 at 1422.751 kb on - strand at 1422.804 kb on + strand at 1422.812 kb on - strand at 1422.827 kb on + strand at 1422.904 kb on - strand at 1422.929 kb on + strand at 1423.056 kb on + strand at 1423.086 kb on + strand, within Dsui_1369 at 1423.108 kb on + strand, within Dsui_1369 at 1423.115 kb on + strand at 1423.116 kb on - strand, within Dsui_1369 at 1423.116 kb on - strand at 1423.169 kb on + strand at 1423.202 kb on + strand at 1423.217 kb on - strand at 1423.224 kb on - strand at 1423.297 kb on + strand at 1423.369 kb on + strand at 1423.472 kb on - strand at 1423.496 kb on + strand, within Dsui_1370 at 1423.557 kb on + strand at 1423.565 kb on - strand, within Dsui_1370 at 1423.569 kb on - strand, within Dsui_1370 at 1423.613 kb on - strand, within Dsui_1370 at 1423.641 kb on - strand, within Dsui_1370 at 1423.772 kb on - strand at 1423.828 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with copper (II) chloride 0.6 mM remove 1,421,588 - +0.8 1,421,609 + +0.7 1,421,609 + -3.3 1,421,609 + -3.0 1,421,609 + +0.8 1,421,609 + -2.5 1,421,617 - +0.8 1,421,617 - +0.8 1,421,624 + -2.6 1,421,624 - -1.2 1,421,632 - -0.8 1,421,709 + Dsui_1367 0.20 -2.1 1,421,721 + Dsui_1367 0.23 -1.0 1,421,724 - Dsui_1367 0.24 -1.5 1,421,724 - +0.8 1,421,780 + Dsui_1367 0.38 -1.5 1,421,814 + Dsui_1367 0.47 -1.8 1,421,815 - -0.2 1,421,826 + Dsui_1367 0.50 -0.2 1,421,947 - Dsui_1367 0.81 +0.8 1,422,015 + -0.2 1,422,092 - -0.2 1,422,103 - +0.8 1,422,110 - -1.2 1,422,152 + Dsui_1368 0.15 -2.0 1,422,171 - +0.8 1,422,175 - -0.2 1,422,191 - Dsui_1368 0.19 +0.8 1,422,217 - Dsui_1368 0.22 +0.8 1,422,225 - Dsui_1368 0.23 +0.8 1,422,239 + Dsui_1368 0.25 -1.5 1,422,247 - Dsui_1368 0.26 -0.8 1,422,350 - +0.8 1,422,453 + Dsui_1368 0.51 +0.8 1,422,466 - Dsui_1368 0.52 -1.2 1,422,569 + +0.8 1,422,569 + Dsui_1368 0.64 +0.8 1,422,667 - +0.8 1,422,713 - +0.8 1,422,715 + +0.8 1,422,743 + Dsui_1368 0.85 -0.2 1,422,743 + Dsui_1368 0.85 +0.8 1,422,751 - -0.2 1,422,804 + -1.2 1,422,812 - -0.2 1,422,827 + +0.8 1,422,904 - +0.8 1,422,929 + +0.8 1,423,056 + -0.2 1,423,086 + Dsui_1369 0.58 -0.8 1,423,108 + Dsui_1369 0.66 -0.2 1,423,115 + +0.8 1,423,116 - Dsui_1369 0.68 -0.2 1,423,116 - +0.8 1,423,169 + -0.2 1,423,202 + -0.2 1,423,217 - -0.8 1,423,224 - -1.2 1,423,297 + +0.8 1,423,369 + -0.2 1,423,472 - +0.8 1,423,496 + Dsui_1370 0.16 +0.8 1,423,557 + +0.8 1,423,565 - Dsui_1370 0.24 -0.2 1,423,569 - Dsui_1370 0.24 -2.0 1,423,613 - Dsui_1370 0.29 -0.2 1,423,641 - Dsui_1370 0.32 -0.2 1,423,772 - +0.8 1,423,828 - -0.2
Or see this region's nucleotide sequence