Experiment: ALP with copper (II) chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1220 and Dsui_1221 are separated by 163 nucleotides Dsui_1221 and Dsui_1222 are separated by 45 nucleotides Dsui_1222 and Dsui_1223 are separated by 4 nucleotides Dsui_1223 and Dsui_1224 are separated by 10 nucleotides
Dsui_1220: Dsui_1220 - gamma-glutamyl phosphate reductase, at 1,275,398 to 1,276,654
_1220
Dsui_1221: Dsui_1221 - hypothetical protein, at 1,276,818 to 1,277,090
_1221
Dsui_1222: Dsui_1222 - DNA polymerase III, delta subunit, at 1,277,136 to 1,278,137
_1222
Dsui_1223: Dsui_1223 - Rare lipoprotein B, at 1,278,142 to 1,278,648
_1223
Dsui_1224: Dsui_1224 - leucyl-tRNA synthetase, at 1,278,659 to 1,281,328
_1224
Position (kb) 1277
1278
1279 Strain fitness (log2 ratio) -2
-1
0
1 at 1276.691 kb on + strand at 1276.757 kb on - strand at 1276.822 kb on + strand at 1276.830 kb on - strand at 1276.848 kb on + strand, within Dsui_1221 at 1276.920 kb on + strand, within Dsui_1221 at 1276.937 kb on + strand, within Dsui_1221 at 1277.085 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with copper (II) chloride 0.6 mM remove 1,276,691 + -1.9 1,276,757 - +0.7 1,276,822 + +0.2 1,276,830 - +0.1 1,276,848 + Dsui_1221 0.11 +0.3 1,276,920 + Dsui_1221 0.37 +0.1 1,276,937 + Dsui_1221 0.44 -0.4 1,277,085 + -0.7
Or see this region's nucleotide sequence