Experiment: ALP with copper (II) chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1031 and Dsui_1032 are separated by 167 nucleotides Dsui_1032 and Dsui_1033 are separated by 3 nucleotides Dsui_1033 and Dsui_1034 overlap by 4 nucleotides
Dsui_1031: Dsui_1031 - formamidopyrimidine-DNA glycosylase Fpg, at 1,063,698 to 1,064,525
_1031
Dsui_1032: Dsui_1032 - Flp pilus assembly protein TadD, at 1,064,693 to 1,066,432
_1032
Dsui_1033: Dsui_1033 - outer membrane lipoprotein LolB, at 1,066,436 to 1,067,011
_1033
Dsui_1034: Dsui_1034 - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, at 1,067,008 to 1,067,859
_1034
Position (kb)
1064
1065
1066
1067 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1063.742 kb on + strand at 1063.750 kb on - strand at 1063.815 kb on - strand, within Dsui_1031 at 1063.887 kb on + strand, within Dsui_1031 at 1064.017 kb on + strand, within Dsui_1031 at 1064.071 kb on + strand at 1064.089 kb on - strand at 1064.158 kb on - strand, within Dsui_1031 at 1064.184 kb on - strand, within Dsui_1031 at 1064.200 kb on - strand, within Dsui_1031 at 1064.228 kb on + strand, within Dsui_1031 at 1064.371 kb on - strand, within Dsui_1031 at 1064.407 kb on + strand, within Dsui_1031 at 1064.509 kb on - strand at 1064.562 kb on + strand at 1064.569 kb on - strand at 1064.587 kb on - strand at 1064.643 kb on - strand at 1064.648 kb on - strand at 1064.678 kb on - strand at 1064.777 kb on + strand at 1064.825 kb on + strand at 1065.021 kb on + strand, within Dsui_1032 at 1065.162 kb on - strand at 1065.469 kb on + strand, within Dsui_1032 at 1065.762 kb on + strand, within Dsui_1032 at 1066.109 kb on + strand, within Dsui_1032 at 1066.209 kb on + strand, within Dsui_1032 at 1066.272 kb on + strand at 1066.342 kb on + strand at 1066.439 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with copper (II) chloride 0.6 mM remove 1,063,742 + -0.2 1,063,750 - +0.8 1,063,815 - Dsui_1031 0.14 -2.6 1,063,887 + Dsui_1031 0.23 -1.6 1,064,017 + Dsui_1031 0.39 +0.8 1,064,071 + +0.8 1,064,089 - +0.8 1,064,158 - Dsui_1031 0.56 -3.1 1,064,184 - Dsui_1031 0.59 -2.2 1,064,200 - Dsui_1031 0.61 -1.6 1,064,228 + Dsui_1031 0.64 -0.2 1,064,371 - Dsui_1031 0.81 -0.2 1,064,407 + Dsui_1031 0.86 +0.8 1,064,509 - -0.9 1,064,562 + -0.2 1,064,569 - +0.8 1,064,587 - -2.0 1,064,643 - +0.8 1,064,648 - +0.8 1,064,678 - +0.8 1,064,777 + -2.6 1,064,825 + -5.4 1,065,021 + Dsui_1032 0.19 -7.4 1,065,162 - +0.8 1,065,469 + Dsui_1032 0.45 -4.4 1,065,762 + Dsui_1032 0.61 -5.9 1,066,109 + Dsui_1032 0.81 -7.6 1,066,209 + Dsui_1032 0.87 -2.8 1,066,272 + -7.2 1,066,342 + +0.4 1,066,439 - -0.8
Or see this region's nucleotide sequence