Experiment: ALP with copper (II) chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0371 and Dsui_0372 overlap by 4 nucleotides Dsui_0372 and Dsui_0373 are separated by 20 nucleotides Dsui_0373 and Dsui_0374 are separated by 61 nucleotides Dsui_0374 and Dsui_0375 are separated by 49 nucleotides Dsui_0375 and Dsui_0376 are separated by 26 nucleotides Dsui_0376 and Dsui_0377 are separated by 64 nucleotides Dsui_0377 and Dsui_0378 are separated by 172 nucleotides
Dsui_0371: Dsui_0371 - transcription termination/antitermination factor NusG, at 371,566 to 372,099
_0371
Dsui_0372: Dsui_0372 - preprotein translocase, SecE subunit, at 372,096 to 372,449
_0372
Dsui_0373: Dsui_0373 - tRNA-Trp, at 372,470 to 372,545
_0373
Dsui_0374: Dsui_0374 - translation elongation factor TU, at 372,607 to 373,797
_0374
Dsui_0375: Dsui_0375 - tRNA-Thr, at 373,847 to 373,921
_0375
Dsui_0376: Dsui_0376 - tRNA-Gly, at 373,948 to 374,021
_0376
Dsui_0377: Dsui_0377 - tRNA-Tyr, at 374,086 to 374,170
_0377
Dsui_0378: Dsui_0378 - FAD/FMN-dependent dehydrogenase, at 374,343 to 375,740
_0378
Position (kb)
372
373
374 Strain fitness (log2 ratio)
-2
-1
0
1 at 374.217 kb on - strand at 374.312 kb on - strand at 374.327 kb on + strand at 374.340 kb on + strand at 374.378 kb on + strand at 374.378 kb on + strand at 374.378 kb on + strand at 374.386 kb on - strand at 374.386 kb on - strand at 374.477 kb on + strand at 374.589 kb on + strand, within Dsui_0378 at 374.597 kb on - strand, within Dsui_0378
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with copper (II) chloride 0.6 mM remove 374,217 - -0.2 374,312 - -1.5 374,327 + +0.8 374,340 + -1.5 374,378 + -0.2 374,378 + -2.0 374,378 + -0.2 374,386 - +0.8 374,386 - +0.8 374,477 + -1.5 374,589 + Dsui_0378 0.18 -1.6 374,597 - Dsui_0378 0.18 -0.8
Or see this region's nucleotide sequence