Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08765

Experiment: pyruvate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08760 and BWI76_RS08765 overlap by 4 nucleotidesBWI76_RS08765 and BWI76_RS08770 overlap by 17 nucleotidesBWI76_RS08770 and BWI76_RS08775 overlap by 8 nucleotides BWI76_RS08760: BWI76_RS08760 - biotin synthase, at 1,846,413 to 1,847,453 _RS08760 BWI76_RS08765: BWI76_RS08765 - 8-amino-7-oxononanoate synthase, at 1,847,450 to 1,848,607 _RS08765 BWI76_RS08770: BWI76_RS08770 - malonyl-ACP O-methyltransferase BioC, at 1,848,591 to 1,849,346 _RS08770 BWI76_RS08775: BWI76_RS08775 - dethiobiotin synthase, at 1,849,339 to 1,850,061 _RS08775 Position (kb) 1847 1848 1849Strain fitness (log2 ratio) -2 -1 0 1at 1846.536 kb on - strand, within BWI76_RS08760at 1846.653 kb on + strand, within BWI76_RS08760at 1846.747 kb on + strand, within BWI76_RS08760at 1846.872 kb on + strand, within BWI76_RS08760at 1846.937 kb on - strand, within BWI76_RS08760at 1847.691 kb on + strand, within BWI76_RS08765at 1847.826 kb on - strand, within BWI76_RS08765at 1848.089 kb on + strand, within BWI76_RS08765at 1848.463 kb on + strand, within BWI76_RS08765at 1848.539 kb on - strandat 1848.691 kb on + strand, within BWI76_RS08770at 1848.740 kb on + strand, within BWI76_RS08770at 1848.968 kb on + strand, within BWI76_RS08770at 1849.437 kb on - strand, within BWI76_RS08775at 1849.551 kb on + strand, within BWI76_RS08775at 1849.559 kb on - strand, within BWI76_RS08775

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Per-strain Table

Position Strand Gene LocusTag Fraction pyruvate (C)
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1,846,536 - BWI76_RS08760 0.12 -0.2
1,846,653 + BWI76_RS08760 0.23 +0.3
1,846,747 + BWI76_RS08760 0.32 -0.4
1,846,872 + BWI76_RS08760 0.44 +1.3
1,846,937 - BWI76_RS08760 0.50 -0.0
1,847,691 + BWI76_RS08765 0.21 +0.7
1,847,826 - BWI76_RS08765 0.32 +0.2
1,848,089 + BWI76_RS08765 0.55 +1.1
1,848,463 + BWI76_RS08765 0.87 -0.5
1,848,539 - -0.0
1,848,691 + BWI76_RS08770 0.13 -2.0
1,848,740 + BWI76_RS08770 0.20 -0.1
1,848,968 + BWI76_RS08770 0.50 +0.0
1,849,437 - BWI76_RS08775 0.14 +0.7
1,849,551 + BWI76_RS08775 0.29 +0.2
1,849,559 - BWI76_RS08775 0.30 +0.3

Or see this region's nucleotide sequence