Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01290 and AZOBR_RS01295 are separated by 121 nucleotides AZOBR_RS01295 and AZOBR_RS01300 overlap by 46 nucleotides AZOBR_RS01300 and AZOBR_RS01305 are separated by 148 nucleotides AZOBR_RS01305 and AZOBR_RS01310 are separated by 93 nucleotides
AZOBR_RS01290: AZOBR_RS01290 - 2-octaprenylphenol hydroxylase, at 266,941 to 268,479
_RS01290
AZOBR_RS01295: AZOBR_RS01295 - hypothetical protein, at 268,601 to 269,332
_RS01295
AZOBR_RS01300: AZOBR_RS01300 - peptide ABC transporter substrate-binding protein, at 269,287 to 271,095
_RS01300
AZOBR_RS01305: AZOBR_RS01305 - inorganic pyrophosphatase, at 271,244 to 271,801
_RS01305
AZOBR_RS01310: AZOBR_RS01310 - lactate dehydrogenase, at 271,895 to 272,836
_RS01310
Position (kb) 269
270
271
272 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 268.477 kb on + strand at 268.478 kb on - strand at 268.478 kb on - strand at 268.478 kb on - strand at 268.478 kb on - strand at 268.634 kb on + strand at 268.634 kb on + strand at 268.635 kb on - strand at 268.635 kb on - strand at 268.635 kb on - strand at 268.886 kb on + strand, within AZOBR_RS01295 at 268.886 kb on + strand, within AZOBR_RS01295 at 268.925 kb on + strand, within AZOBR_RS01295 at 268.926 kb on - strand, within AZOBR_RS01295 at 269.189 kb on + strand, within AZOBR_RS01295 at 269.190 kb on - strand, within AZOBR_RS01295 at 269.330 kb on + strand at 269.411 kb on + strand at 269.411 kb on + strand at 269.411 kb on + strand at 269.411 kb on + strand at 269.411 kb on + strand at 269.411 kb on + strand at 269.412 kb on - strand at 269.412 kb on - strand at 269.412 kb on - strand at 269.412 kb on - strand at 269.412 kb on - strand at 269.412 kb on - strand at 269.412 kb on - strand at 269.412 kb on - strand at 269.531 kb on + strand, within AZOBR_RS01300 at 269.573 kb on + strand, within AZOBR_RS01300 at 269.573 kb on + strand, within AZOBR_RS01300 at 269.573 kb on + strand, within AZOBR_RS01300 at 269.573 kb on + strand, within AZOBR_RS01300 at 269.574 kb on - strand, within AZOBR_RS01300 at 269.574 kb on - strand, within AZOBR_RS01300 at 269.574 kb on - strand, within AZOBR_RS01300 at 269.576 kb on + strand, within AZOBR_RS01300 at 269.576 kb on + strand, within AZOBR_RS01300 at 269.577 kb on - strand, within AZOBR_RS01300 at 269.577 kb on - strand, within AZOBR_RS01300 at 269.863 kb on - strand, within AZOBR_RS01300 at 270.014 kb on + strand, within AZOBR_RS01300 at 270.014 kb on + strand, within AZOBR_RS01300 at 270.017 kb on + strand, within AZOBR_RS01300 at 270.017 kb on + strand, within AZOBR_RS01300 at 270.017 kb on + strand, within AZOBR_RS01300 at 270.017 kb on + strand, within AZOBR_RS01300 at 270.018 kb on - strand, within AZOBR_RS01300 at 270.018 kb on - strand, within AZOBR_RS01300 at 270.314 kb on + strand, within AZOBR_RS01300 at 270.314 kb on + strand, within AZOBR_RS01300 at 270.315 kb on - strand, within AZOBR_RS01300 at 270.377 kb on + strand, within AZOBR_RS01300 at 270.378 kb on - strand, within AZOBR_RS01300 at 270.626 kb on + strand, within AZOBR_RS01300 at 270.626 kb on + strand, within AZOBR_RS01300 at 270.626 kb on + strand, within AZOBR_RS01300 at 270.795 kb on - strand, within AZOBR_RS01300 at 270.983 kb on + strand at 271.142 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days remove 268,477 + +0.2 268,478 - -0.3 268,478 - +0.2 268,478 - +0.8 268,478 - +0.3 268,634 + -0.1 268,634 + +0.1 268,635 - +0.9 268,635 - +0.5 268,635 - +0.2 268,886 + AZOBR_RS01295 0.39 -0.3 268,886 + AZOBR_RS01295 0.39 +0.2 268,925 + AZOBR_RS01295 0.44 +0.2 268,926 - AZOBR_RS01295 0.44 -0.2 269,189 + AZOBR_RS01295 0.80 +2.4 269,190 - AZOBR_RS01295 0.80 +0.6 269,330 + +0.1 269,411 + +0.1 269,411 + +0.1 269,411 + +0.2 269,411 + -2.1 269,411 + -0.2 269,411 + -0.1 269,412 - -0.2 269,412 - +0.1 269,412 - +0.5 269,412 - -0.7 269,412 - +0.9 269,412 - -1.0 269,412 - +0.7 269,412 - +0.6 269,531 + AZOBR_RS01300 0.13 +0.5 269,573 + AZOBR_RS01300 0.16 +1.1 269,573 + AZOBR_RS01300 0.16 +0.0 269,573 + AZOBR_RS01300 0.16 -0.4 269,573 + AZOBR_RS01300 0.16 -1.3 269,574 - AZOBR_RS01300 0.16 -1.1 269,574 - AZOBR_RS01300 0.16 -0.4 269,574 - AZOBR_RS01300 0.16 -0.1 269,576 + AZOBR_RS01300 0.16 +0.3 269,576 + AZOBR_RS01300 0.16 -2.0 269,577 - AZOBR_RS01300 0.16 +1.4 269,577 - AZOBR_RS01300 0.16 +0.6 269,863 - AZOBR_RS01300 0.32 -0.2 270,014 + AZOBR_RS01300 0.40 +1.6 270,014 + AZOBR_RS01300 0.40 +0.8 270,017 + AZOBR_RS01300 0.40 +1.9 270,017 + AZOBR_RS01300 0.40 -1.4 270,017 + AZOBR_RS01300 0.40 +1.3 270,017 + AZOBR_RS01300 0.40 -0.3 270,018 - AZOBR_RS01300 0.40 -0.4 270,018 - AZOBR_RS01300 0.40 -1.5 270,314 + AZOBR_RS01300 0.57 +0.3 270,314 + AZOBR_RS01300 0.57 +0.4 270,315 - AZOBR_RS01300 0.57 +0.6 270,377 + AZOBR_RS01300 0.60 +0.1 270,378 - AZOBR_RS01300 0.60 +1.3 270,626 + AZOBR_RS01300 0.74 +0.2 270,626 + AZOBR_RS01300 0.74 -1.1 270,626 + AZOBR_RS01300 0.74 +0.7 270,795 - AZOBR_RS01300 0.83 +1.1 270,983 + -3.6 271,142 + +1.6
Or see this region's nucleotide sequence