Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35435 and RR42_RS35440 are separated by 12 nucleotides RR42_RS35440 and RR42_RS35445 are separated by 84 nucleotides RR42_RS35445 and RR42_RS35450 are separated by 10 nucleotides RR42_RS35450 and RR42_RS35455 are separated by 1 nucleotides
RR42_RS35435: RR42_RS35435 - NAD(FAD)-dependent dehydrogenase, at 3,402,588 to 3,404,255
_RS35435
RR42_RS35440: RR42_RS35440 - MBL fold metallo-hydrolase, at 3,404,268 to 3,405,128
_RS35440
RR42_RS35445: RR42_RS35445 - ArsR family transcriptional regulator, at 3,405,213 to 3,405,536
_RS35445
RR42_RS35450: RR42_RS35450 - hypothetical protein, at 3,405,547 to 3,405,981
_RS35450
RR42_RS35455: RR42_RS35455 - membrane protein, at 3,405,983 to 3,406,414
_RS35455
Position (kb)
3404
3405
3406 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3403.295 kb on + strand, within RR42_RS35435 at 3403.295 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.310 kb on - strand, within RR42_RS35435 at 3403.310 kb on - strand, within RR42_RS35435 at 3403.310 kb on - strand, within RR42_RS35435 at 3403.310 kb on - strand, within RR42_RS35435 at 3403.409 kb on - strand, within RR42_RS35435 at 3403.509 kb on + strand, within RR42_RS35435 at 3403.525 kb on + strand, within RR42_RS35435 at 3403.693 kb on + strand, within RR42_RS35435 at 3403.694 kb on - strand, within RR42_RS35435 at 3403.696 kb on + strand, within RR42_RS35435 at 3403.697 kb on - strand, within RR42_RS35435 at 3403.732 kb on - strand, within RR42_RS35435 at 3403.860 kb on + strand, within RR42_RS35435 at 3403.861 kb on - strand, within RR42_RS35435 at 3403.905 kb on + strand, within RR42_RS35435 at 3403.988 kb on + strand, within RR42_RS35435 at 3403.988 kb on + strand, within RR42_RS35435 at 3404.194 kb on - strand at 3404.260 kb on + strand at 3404.260 kb on + strand at 3404.261 kb on - strand at 3404.261 kb on - strand at 3404.261 kb on - strand at 3404.472 kb on + strand, within RR42_RS35440 at 3404.575 kb on + strand, within RR42_RS35440 at 3404.576 kb on - strand, within RR42_RS35440 at 3404.576 kb on - strand, within RR42_RS35440 at 3404.675 kb on - strand, within RR42_RS35440 at 3404.692 kb on - strand, within RR42_RS35440 at 3404.693 kb on + strand, within RR42_RS35440 at 3404.693 kb on + strand, within RR42_RS35440 at 3404.693 kb on + strand, within RR42_RS35440 at 3404.694 kb on - strand, within RR42_RS35440 at 3404.888 kb on - strand, within RR42_RS35440 at 3404.888 kb on - strand, within RR42_RS35440 at 3405.019 kb on + strand, within RR42_RS35440 at 3405.020 kb on - strand, within RR42_RS35440 at 3405.020 kb on - strand, within RR42_RS35440 at 3405.068 kb on - strand at 3405.076 kb on + strand at 3405.076 kb on + strand at 3405.076 kb on + strand at 3405.077 kb on - strand at 3405.077 kb on - strand at 3405.077 kb on - strand at 3405.156 kb on + strand at 3405.157 kb on - strand at 3405.328 kb on + strand, within RR42_RS35445 at 3405.328 kb on + strand, within RR42_RS35445 at 3405.462 kb on + strand, within RR42_RS35445 at 3405.463 kb on - strand, within RR42_RS35445 at 3405.463 kb on - strand, within RR42_RS35445 at 3405.534 kb on + strand at 3405.535 kb on - strand at 3405.535 kb on - strand at 3405.546 kb on + strand at 3405.546 kb on + strand at 3405.546 kb on + strand at 3405.547 kb on - strand at 3405.564 kb on + strand at 3405.783 kb on + strand, within RR42_RS35450 at 3405.875 kb on + strand, within RR42_RS35450 at 3405.957 kb on - strand at 3406.066 kb on - strand, within RR42_RS35455
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,403,295 + RR42_RS35435 0.42 +1.3 3,403,295 + RR42_RS35435 0.42 +1.0 3,403,309 + RR42_RS35435 0.43 -1.6 3,403,309 + RR42_RS35435 0.43 +0.8 3,403,309 + RR42_RS35435 0.43 +0.3 3,403,309 + RR42_RS35435 0.43 -1.3 3,403,309 + RR42_RS35435 0.43 +0.4 3,403,309 + RR42_RS35435 0.43 -1.1 3,403,309 + RR42_RS35435 0.43 -0.2 3,403,310 - RR42_RS35435 0.43 -0.2 3,403,310 - RR42_RS35435 0.43 -0.2 3,403,310 - RR42_RS35435 0.43 +0.3 3,403,310 - RR42_RS35435 0.43 -0.8 3,403,409 - RR42_RS35435 0.49 +0.6 3,403,509 + RR42_RS35435 0.55 -0.7 3,403,525 + RR42_RS35435 0.56 -0.8 3,403,693 + RR42_RS35435 0.66 +0.0 3,403,694 - RR42_RS35435 0.66 -0.1 3,403,696 + RR42_RS35435 0.66 -0.7 3,403,697 - RR42_RS35435 0.66 -0.4 3,403,732 - RR42_RS35435 0.69 +0.0 3,403,860 + RR42_RS35435 0.76 +0.0 3,403,861 - RR42_RS35435 0.76 -1.2 3,403,905 + RR42_RS35435 0.79 +0.6 3,403,988 + RR42_RS35435 0.84 -1.5 3,403,988 + RR42_RS35435 0.84 -0.2 3,404,194 - -1.0 3,404,260 + +0.6 3,404,260 + -2.2 3,404,261 - -0.4 3,404,261 - +0.3 3,404,261 - +0.1 3,404,472 + RR42_RS35440 0.24 +1.3 3,404,575 + RR42_RS35440 0.36 -0.4 3,404,576 - RR42_RS35440 0.36 -0.6 3,404,576 - RR42_RS35440 0.36 +0.3 3,404,675 - RR42_RS35440 0.47 +1.2 3,404,692 - RR42_RS35440 0.49 -1.3 3,404,693 + RR42_RS35440 0.49 -0.4 3,404,693 + RR42_RS35440 0.49 -1.7 3,404,693 + RR42_RS35440 0.49 -0.6 3,404,694 - RR42_RS35440 0.49 -0.7 3,404,888 - RR42_RS35440 0.72 -2.0 3,404,888 - RR42_RS35440 0.72 -0.2 3,405,019 + RR42_RS35440 0.87 -0.2 3,405,020 - RR42_RS35440 0.87 -2.9 3,405,020 - RR42_RS35440 0.87 -1.1 3,405,068 - +0.6 3,405,076 + -1.9 3,405,076 + +0.7 3,405,076 + -1.2 3,405,077 - +1.5 3,405,077 - +0.7 3,405,077 - +0.0 3,405,156 + -1.3 3,405,157 - +0.4 3,405,328 + RR42_RS35445 0.35 +1.3 3,405,328 + RR42_RS35445 0.35 +0.7 3,405,462 + RR42_RS35445 0.77 +1.2 3,405,463 - RR42_RS35445 0.77 +0.8 3,405,463 - RR42_RS35445 0.77 +2.0 3,405,534 + +0.9 3,405,535 - +1.1 3,405,535 - -0.0 3,405,546 + -0.5 3,405,546 + -0.0 3,405,546 + +1.5 3,405,547 - -0.5 3,405,564 + +0.7 3,405,783 + RR42_RS35450 0.54 -0.2 3,405,875 + RR42_RS35450 0.75 -1.5 3,405,957 - -0.5 3,406,066 - RR42_RS35455 0.19 +1.6
Or see this region's nucleotide sequence