Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35320 and RR42_RS35325 overlap by 4 nucleotides RR42_RS35325 and RR42_RS35330 are separated by 291 nucleotides RR42_RS35330 and RR42_RS35335 are separated by 229 nucleotides RR42_RS35335 and RR42_RS35340 are separated by 229 nucleotides
RR42_RS35320: RR42_RS35320 - membrane protein, at 3,378,579 to 3,379,421
_RS35320
RR42_RS35325: RR42_RS35325 - hypothetical protein, at 3,379,418 to 3,380,038
_RS35325
RR42_RS35330: RR42_RS35330 - S-adenosylmethionine synthetase, at 3,380,330 to 3,381,511
_RS35330
RR42_RS35335: RR42_RS35335 - hypothetical protein, at 3,381,741 to 3,382,142
_RS35335
RR42_RS35340: RR42_RS35340 - cell division protein FtsH, at 3,382,372 to 3,384,315
_RS35340
Position (kb)
3380
3381
3382 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3379.387 kb on - strand at 3379.387 kb on - strand at 3379.517 kb on + strand, within RR42_RS35325 at 3379.517 kb on + strand, within RR42_RS35325 at 3379.518 kb on - strand, within RR42_RS35325 at 3379.522 kb on + strand, within RR42_RS35325 at 3379.524 kb on + strand, within RR42_RS35325 at 3379.524 kb on + strand, within RR42_RS35325 at 3379.525 kb on - strand, within RR42_RS35325 at 3379.525 kb on - strand, within RR42_RS35325 at 3379.525 kb on - strand, within RR42_RS35325 at 3379.528 kb on + strand, within RR42_RS35325 at 3379.529 kb on - strand, within RR42_RS35325 at 3379.587 kb on - strand, within RR42_RS35325 at 3379.597 kb on + strand, within RR42_RS35325 at 3379.597 kb on + strand, within RR42_RS35325 at 3379.597 kb on + strand, within RR42_RS35325 at 3379.597 kb on + strand, within RR42_RS35325 at 3379.597 kb on + strand, within RR42_RS35325 at 3379.598 kb on - strand, within RR42_RS35325 at 3379.598 kb on - strand, within RR42_RS35325 at 3379.598 kb on - strand, within RR42_RS35325 at 3379.598 kb on - strand, within RR42_RS35325 at 3379.599 kb on + strand, within RR42_RS35325 at 3379.617 kb on + strand, within RR42_RS35325 at 3379.690 kb on - strand, within RR42_RS35325 at 3379.690 kb on - strand, within RR42_RS35325 at 3379.876 kb on + strand, within RR42_RS35325 at 3379.893 kb on - strand, within RR42_RS35325 at 3379.975 kb on + strand, within RR42_RS35325 at 3379.978 kb on - strand at 3379.978 kb on - strand at 3379.978 kb on - strand at 3379.978 kb on - strand at 3379.978 kb on - strand at 3380.010 kb on - strand at 3380.028 kb on - strand at 3380.089 kb on + strand at 3380.098 kb on - strand at 3380.143 kb on + strand at 3380.144 kb on - strand at 3380.151 kb on + strand at 3380.151 kb on + strand at 3380.151 kb on + strand at 3380.151 kb on + strand at 3380.155 kb on - strand at 3380.155 kb on - strand at 3380.240 kb on - strand at 3380.288 kb on + strand at 3380.288 kb on + strand at 3380.288 kb on + strand at 3380.288 kb on + strand at 3380.416 kb on + strand at 3380.435 kb on - strand at 3380.473 kb on - strand, within RR42_RS35330 at 3380.483 kb on + strand, within RR42_RS35330 at 3380.598 kb on + strand, within RR42_RS35330 at 3380.669 kb on + strand, within RR42_RS35330 at 3380.701 kb on + strand, within RR42_RS35330 at 3380.702 kb on - strand, within RR42_RS35330 at 3380.805 kb on - strand, within RR42_RS35330 at 3380.805 kb on - strand, within RR42_RS35330 at 3380.814 kb on - strand, within RR42_RS35330 at 3380.903 kb on + strand, within RR42_RS35330 at 3380.903 kb on + strand, within RR42_RS35330 at 3380.903 kb on + strand, within RR42_RS35330 at 3380.993 kb on + strand, within RR42_RS35330 at 3380.994 kb on - strand, within RR42_RS35330 at 3380.994 kb on - strand, within RR42_RS35330 at 3380.994 kb on - strand, within RR42_RS35330 at 3381.016 kb on + strand, within RR42_RS35330 at 3381.067 kb on + strand, within RR42_RS35330 at 3381.187 kb on + strand, within RR42_RS35330 at 3381.188 kb on - strand, within RR42_RS35330 at 3381.188 kb on - strand, within RR42_RS35330 at 3381.258 kb on - strand, within RR42_RS35330 at 3381.509 kb on + strand at 3381.509 kb on + strand at 3381.563 kb on + strand at 3381.718 kb on - strand at 3381.718 kb on - strand at 3381.766 kb on - strand at 3381.778 kb on - strand at 3381.778 kb on - strand at 3381.778 kb on - strand at 3381.925 kb on - strand, within RR42_RS35335 at 3382.035 kb on + strand, within RR42_RS35335 at 3382.035 kb on + strand, within RR42_RS35335 at 3382.038 kb on + strand, within RR42_RS35335 at 3382.038 kb on + strand, within RR42_RS35335 at 3382.039 kb on - strand, within RR42_RS35335 at 3382.039 kb on - strand, within RR42_RS35335 at 3382.161 kb on - strand at 3382.225 kb on + strand at 3382.226 kb on - strand at 3382.279 kb on + strand at 3382.305 kb on + strand at 3382.306 kb on - strand at 3382.351 kb on + strand at 3382.359 kb on + strand at 3382.360 kb on - strand at 3382.405 kb on + strand at 3382.405 kb on + strand at 3382.405 kb on + strand at 3382.406 kb on - strand at 3382.409 kb on + strand at 3382.410 kb on - strand at 3382.410 kb on - strand at 3382.410 kb on - strand at 3382.410 kb on - strand at 3382.410 kb on - strand at 3382.410 kb on - strand at 3382.448 kb on - strand at 3382.448 kb on - strand at 3382.448 kb on - strand at 3382.448 kb on - strand at 3382.451 kb on + strand at 3382.451 kb on + strand at 3382.451 kb on + strand at 3382.451 kb on + strand at 3382.452 kb on - strand at 3382.452 kb on - strand at 3382.481 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,379,387 - -1.6 3,379,387 - +0.6 3,379,517 + RR42_RS35325 0.16 -1.2 3,379,517 + RR42_RS35325 0.16 -0.4 3,379,518 - RR42_RS35325 0.16 +1.6 3,379,522 + RR42_RS35325 0.17 +0.0 3,379,524 + RR42_RS35325 0.17 -0.0 3,379,524 + RR42_RS35325 0.17 -0.0 3,379,525 - RR42_RS35325 0.17 -1.5 3,379,525 - RR42_RS35325 0.17 -1.5 3,379,525 - RR42_RS35325 0.17 +0.1 3,379,528 + RR42_RS35325 0.18 +0.8 3,379,529 - RR42_RS35325 0.18 -1.7 3,379,587 - RR42_RS35325 0.27 -0.2 3,379,597 + RR42_RS35325 0.29 +0.3 3,379,597 + RR42_RS35325 0.29 +0.4 3,379,597 + RR42_RS35325 0.29 -0.1 3,379,597 + RR42_RS35325 0.29 -0.2 3,379,597 + RR42_RS35325 0.29 +0.5 3,379,598 - RR42_RS35325 0.29 -0.5 3,379,598 - RR42_RS35325 0.29 -0.8 3,379,598 - RR42_RS35325 0.29 -0.4 3,379,598 - RR42_RS35325 0.29 -2.5 3,379,599 + RR42_RS35325 0.29 -0.2 3,379,617 + RR42_RS35325 0.32 -0.1 3,379,690 - RR42_RS35325 0.44 +0.1 3,379,690 - RR42_RS35325 0.44 +1.6 3,379,876 + RR42_RS35325 0.74 +0.7 3,379,893 - RR42_RS35325 0.76 +0.8 3,379,975 + RR42_RS35325 0.90 +0.7 3,379,978 - +1.0 3,379,978 - +0.5 3,379,978 - -0.5 3,379,978 - +0.2 3,379,978 - -0.6 3,380,010 - -1.1 3,380,028 - +0.1 3,380,089 + -0.8 3,380,098 - +0.0 3,380,143 + -1.2 3,380,144 - +0.7 3,380,151 + -0.0 3,380,151 + -0.1 3,380,151 + +0.2 3,380,151 + -1.5 3,380,155 - +0.0 3,380,155 - +0.8 3,380,240 - +0.0 3,380,288 + -0.3 3,380,288 + -0.3 3,380,288 + +0.1 3,380,288 + +2.1 3,380,416 + +1.0 3,380,435 - +2.3 3,380,473 - RR42_RS35330 0.12 -0.2 3,380,483 + RR42_RS35330 0.13 -0.1 3,380,598 + RR42_RS35330 0.23 +0.2 3,380,669 + RR42_RS35330 0.29 -0.2 3,380,701 + RR42_RS35330 0.31 +0.6 3,380,702 - RR42_RS35330 0.31 -0.8 3,380,805 - RR42_RS35330 0.40 +0.3 3,380,805 - RR42_RS35330 0.40 -0.0 3,380,814 - RR42_RS35330 0.41 -0.3 3,380,903 + RR42_RS35330 0.48 +0.6 3,380,903 + RR42_RS35330 0.48 +2.1 3,380,903 + RR42_RS35330 0.48 +0.5 3,380,993 + RR42_RS35330 0.56 +0.3 3,380,994 - RR42_RS35330 0.56 -0.5 3,380,994 - RR42_RS35330 0.56 -0.0 3,380,994 - RR42_RS35330 0.56 -0.7 3,381,016 + RR42_RS35330 0.58 +0.4 3,381,067 + RR42_RS35330 0.62 -1.4 3,381,187 + RR42_RS35330 0.73 -1.1 3,381,188 - RR42_RS35330 0.73 -0.4 3,381,188 - RR42_RS35330 0.73 -0.8 3,381,258 - RR42_RS35330 0.79 -0.2 3,381,509 + +0.6 3,381,509 + -1.1 3,381,563 + +1.8 3,381,718 - -1.3 3,381,718 - -1.6 3,381,766 - +1.5 3,381,778 - -1.1 3,381,778 - +0.6 3,381,778 - -0.6 3,381,925 - RR42_RS35335 0.46 +0.2 3,382,035 + RR42_RS35335 0.73 -1.9 3,382,035 + RR42_RS35335 0.73 -0.8 3,382,038 + RR42_RS35335 0.74 +0.4 3,382,038 + RR42_RS35335 0.74 -0.5 3,382,039 - RR42_RS35335 0.74 -1.1 3,382,039 - RR42_RS35335 0.74 -0.4 3,382,161 - -0.0 3,382,225 + -0.4 3,382,226 - +0.5 3,382,279 + -0.2 3,382,305 + -0.6 3,382,306 - +0.4 3,382,351 + -0.4 3,382,359 + -0.6 3,382,360 - -1.8 3,382,405 + -0.9 3,382,405 + +0.4 3,382,405 + +0.0 3,382,406 - +2.0 3,382,409 + +0.5 3,382,410 - +1.1 3,382,410 - -0.6 3,382,410 - -0.9 3,382,410 - +0.6 3,382,410 - -0.6 3,382,410 - +0.1 3,382,448 - +0.4 3,382,448 - -0.3 3,382,448 - +0.3 3,382,448 - -0.4 3,382,451 + +0.5 3,382,451 + +0.2 3,382,451 + -0.5 3,382,451 + -2.1 3,382,452 - +1.4 3,382,452 - +0.9 3,382,481 - -0.4
Or see this region's nucleotide sequence