Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34065 and RR42_RS34070 are separated by 56 nucleotides RR42_RS34070 and RR42_RS34075 are separated by 293 nucleotides RR42_RS34075 and RR42_RS34080 are separated by 83 nucleotides
RR42_RS34065: RR42_RS34065 - quinone oxidoreductase, at 3,072,009 to 3,072,992
_RS34065
RR42_RS34070: RR42_RS34070 - NADPH:quinone oxidoreductase, at 3,073,049 to 3,074,050
_RS34070
RR42_RS34075: RR42_RS34075 - 4-oxalocrotonate tautomerase, at 3,074,344 to 3,074,724
_RS34075
RR42_RS34080: RR42_RS34080 - LysR family transcriptional regulator, at 3,074,808 to 3,075,665
_RS34080
Position (kb)
3073
3074
3075 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3072.057 kb on + strand at 3072.057 kb on + strand at 3072.058 kb on - strand at 3072.058 kb on - strand at 3072.058 kb on - strand at 3072.301 kb on - strand, within RR42_RS34065 at 3072.301 kb on - strand, within RR42_RS34065 at 3072.498 kb on + strand, within RR42_RS34065 at 3072.499 kb on - strand, within RR42_RS34065 at 3072.586 kb on - strand, within RR42_RS34065 at 3072.586 kb on - strand, within RR42_RS34065 at 3072.640 kb on + strand, within RR42_RS34065 at 3072.641 kb on - strand, within RR42_RS34065 at 3072.641 kb on - strand, within RR42_RS34065 at 3072.802 kb on - strand, within RR42_RS34065 at 3072.813 kb on + strand, within RR42_RS34065 at 3072.884 kb on - strand, within RR42_RS34065 at 3072.884 kb on - strand, within RR42_RS34065 at 3072.884 kb on - strand, within RR42_RS34065 at 3072.965 kb on + strand at 3073.020 kb on - strand at 3073.020 kb on - strand at 3073.025 kb on - strand at 3073.246 kb on + strand, within RR42_RS34070 at 3073.573 kb on + strand, within RR42_RS34070 at 3073.579 kb on + strand, within RR42_RS34070 at 3073.579 kb on + strand, within RR42_RS34070 at 3073.631 kb on + strand, within RR42_RS34070 at 3073.632 kb on - strand, within RR42_RS34070 at 3073.633 kb on + strand, within RR42_RS34070 at 3073.633 kb on + strand, within RR42_RS34070 at 3073.815 kb on + strand, within RR42_RS34070 at 3073.815 kb on + strand, within RR42_RS34070 at 3073.816 kb on - strand, within RR42_RS34070 at 3073.866 kb on + strand, within RR42_RS34070 at 3073.867 kb on - strand, within RR42_RS34070 at 3073.871 kb on + strand, within RR42_RS34070 at 3073.871 kb on + strand, within RR42_RS34070 at 3073.931 kb on - strand, within RR42_RS34070 at 3073.931 kb on - strand, within RR42_RS34070 at 3073.931 kb on - strand, within RR42_RS34070 at 3074.001 kb on - strand at 3074.069 kb on - strand at 3074.069 kb on - strand at 3074.069 kb on - strand at 3074.069 kb on - strand at 3074.147 kb on - strand at 3074.147 kb on - strand at 3074.223 kb on + strand at 3074.351 kb on + strand at 3074.351 kb on + strand at 3074.351 kb on + strand at 3074.352 kb on - strand at 3074.488 kb on + strand, within RR42_RS34075 at 3074.488 kb on + strand, within RR42_RS34075 at 3074.488 kb on + strand, within RR42_RS34075 at 3074.489 kb on - strand, within RR42_RS34075 at 3074.520 kb on + strand, within RR42_RS34075 at 3074.521 kb on - strand, within RR42_RS34075 at 3074.555 kb on + strand, within RR42_RS34075 at 3074.555 kb on + strand, within RR42_RS34075 at 3074.595 kb on + strand, within RR42_RS34075 at 3074.596 kb on - strand, within RR42_RS34075 at 3074.596 kb on - strand, within RR42_RS34075 at 3074.598 kb on + strand, within RR42_RS34075 at 3074.598 kb on + strand, within RR42_RS34075 at 3074.598 kb on + strand, within RR42_RS34075 at 3074.755 kb on + strand at 3074.877 kb on - strand at 3074.877 kb on - strand at 3074.877 kb on - strand at 3074.945 kb on + strand, within RR42_RS34080 at 3074.945 kb on + strand, within RR42_RS34080 at 3074.946 kb on - strand, within RR42_RS34080 at 3074.946 kb on - strand, within RR42_RS34080 at 3074.946 kb on - strand, within RR42_RS34080
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,072,057 + +1.6 3,072,057 + -1.6 3,072,058 - -0.1 3,072,058 - -0.1 3,072,058 - -2.2 3,072,301 - RR42_RS34065 0.30 -0.3 3,072,301 - RR42_RS34065 0.30 -0.1 3,072,498 + RR42_RS34065 0.50 -0.2 3,072,499 - RR42_RS34065 0.50 -0.2 3,072,586 - RR42_RS34065 0.59 +0.3 3,072,586 - RR42_RS34065 0.59 +0.6 3,072,640 + RR42_RS34065 0.64 +0.5 3,072,641 - RR42_RS34065 0.64 +0.1 3,072,641 - RR42_RS34065 0.64 -0.4 3,072,802 - RR42_RS34065 0.81 -0.2 3,072,813 + RR42_RS34065 0.82 -0.7 3,072,884 - RR42_RS34065 0.89 +1.6 3,072,884 - RR42_RS34065 0.89 -0.8 3,072,884 - RR42_RS34065 0.89 -0.4 3,072,965 + +1.2 3,073,020 - +1.0 3,073,020 - -0.5 3,073,025 - +0.4 3,073,246 + RR42_RS34070 0.20 -0.3 3,073,573 + RR42_RS34070 0.52 -2.0 3,073,579 + RR42_RS34070 0.53 +0.1 3,073,579 + RR42_RS34070 0.53 +1.1 3,073,631 + RR42_RS34070 0.58 +0.6 3,073,632 - RR42_RS34070 0.58 +0.4 3,073,633 + RR42_RS34070 0.58 -0.4 3,073,633 + RR42_RS34070 0.58 -1.3 3,073,815 + RR42_RS34070 0.76 +0.9 3,073,815 + RR42_RS34070 0.76 +2.6 3,073,816 - RR42_RS34070 0.77 +0.3 3,073,866 + RR42_RS34070 0.82 -1.2 3,073,867 - RR42_RS34070 0.82 +0.1 3,073,871 + RR42_RS34070 0.82 +0.6 3,073,871 + RR42_RS34070 0.82 -1.0 3,073,931 - RR42_RS34070 0.88 -0.5 3,073,931 - RR42_RS34070 0.88 -0.5 3,073,931 - RR42_RS34070 0.88 -1.1 3,074,001 - -0.4 3,074,069 - -0.5 3,074,069 - -1.0 3,074,069 - +0.7 3,074,069 - -0.2 3,074,147 - +0.5 3,074,147 - +0.9 3,074,223 + -0.9 3,074,351 + +0.4 3,074,351 + -1.5 3,074,351 + +0.2 3,074,352 - +0.6 3,074,488 + RR42_RS34075 0.38 -0.7 3,074,488 + RR42_RS34075 0.38 +0.0 3,074,488 + RR42_RS34075 0.38 -0.8 3,074,489 - RR42_RS34075 0.38 +0.0 3,074,520 + RR42_RS34075 0.46 -2.5 3,074,521 - RR42_RS34075 0.46 -0.7 3,074,555 + RR42_RS34075 0.55 +0.3 3,074,555 + RR42_RS34075 0.55 +0.5 3,074,595 + RR42_RS34075 0.66 +0.4 3,074,596 - RR42_RS34075 0.66 -1.3 3,074,596 - RR42_RS34075 0.66 +0.8 3,074,598 + RR42_RS34075 0.67 -0.2 3,074,598 + RR42_RS34075 0.67 -0.5 3,074,598 + RR42_RS34075 0.67 -3.0 3,074,755 + +0.3 3,074,877 - -0.2 3,074,877 - -0.5 3,074,877 - -0.6 3,074,945 + RR42_RS34080 0.16 +1.2 3,074,945 + RR42_RS34080 0.16 -1.1 3,074,946 - RR42_RS34080 0.16 -0.1 3,074,946 - RR42_RS34080 0.16 +0.5 3,074,946 - RR42_RS34080 0.16 +1.4
Or see this region's nucleotide sequence