Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33180 and RR42_RS33185 are separated by 818 nucleotides RR42_RS33185 and RR42_RS33190 are separated by 91 nucleotides
RR42_RS33180: RR42_RS33180 - hypothetical protein, at 2,849,958 to 2,850,740
_RS33180
RR42_RS33185: RR42_RS33185 - AraC family transcriptional regulator, at 2,851,559 to 2,852,506
_RS33185
RR42_RS33190: RR42_RS33190 - transcriptional regulator, at 2,852,598 to 2,853,347
_RS33190
Position (kb)
2851
2852
2853 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2850.597 kb on + strand, within RR42_RS33180 at 2850.672 kb on - strand at 2850.679 kb on + strand at 2850.680 kb on - strand at 2850.691 kb on + strand at 2850.691 kb on + strand at 2850.691 kb on + strand at 2850.692 kb on - strand at 2850.692 kb on - strand at 2850.732 kb on + strand at 2850.732 kb on + strand at 2850.733 kb on - strand at 2850.733 kb on - strand at 2850.733 kb on - strand at 2850.747 kb on - strand at 2850.747 kb on - strand at 2850.762 kb on + strand at 2850.763 kb on - strand at 2850.763 kb on - strand at 2850.763 kb on - strand at 2850.763 kb on - strand at 2850.787 kb on + strand at 2850.791 kb on + strand at 2850.791 kb on + strand at 2850.791 kb on + strand at 2850.791 kb on + strand at 2850.791 kb on + strand at 2850.792 kb on - strand at 2850.795 kb on + strand at 2850.795 kb on + strand at 2850.795 kb on + strand at 2850.796 kb on - strand at 2850.796 kb on - strand at 2850.796 kb on - strand at 2850.796 kb on - strand at 2850.796 kb on - strand at 2850.797 kb on + strand at 2850.797 kb on + strand at 2850.798 kb on - strand at 2850.844 kb on + strand at 2850.847 kb on + strand at 2850.847 kb on + strand at 2850.847 kb on + strand at 2850.848 kb on - strand at 2850.848 kb on - strand at 2850.848 kb on - strand at 2850.938 kb on + strand at 2850.974 kb on + strand at 2850.974 kb on + strand at 2850.974 kb on + strand at 2850.974 kb on + strand at 2851.023 kb on + strand at 2851.025 kb on + strand at 2851.027 kb on + strand at 2851.027 kb on + strand at 2851.027 kb on + strand at 2851.027 kb on + strand at 2851.027 kb on + strand at 2851.028 kb on - strand at 2851.028 kb on - strand at 2851.028 kb on - strand at 2851.028 kb on - strand at 2851.028 kb on - strand at 2851.028 kb on - strand at 2851.028 kb on - strand at 2851.028 kb on - strand at 2851.029 kb on + strand at 2851.029 kb on + strand at 2851.029 kb on + strand at 2851.029 kb on + strand at 2851.030 kb on - strand at 2851.030 kb on - strand at 2851.030 kb on - strand at 2851.180 kb on + strand at 2851.233 kb on + strand at 2851.355 kb on - strand at 2851.355 kb on - strand at 2851.355 kb on - strand at 2851.430 kb on - strand at 2851.475 kb on - strand at 2851.488 kb on + strand at 2851.620 kb on - strand at 2851.715 kb on + strand, within RR42_RS33185 at 2851.715 kb on + strand, within RR42_RS33185 at 2851.716 kb on - strand, within RR42_RS33185 at 2851.716 kb on - strand, within RR42_RS33185 at 2852.212 kb on - strand, within RR42_RS33185 at 2852.212 kb on - strand, within RR42_RS33185 at 2852.213 kb on + strand, within RR42_RS33185 at 2852.249 kb on + strand, within RR42_RS33185 at 2852.249 kb on + strand, within RR42_RS33185 at 2852.249 kb on + strand, within RR42_RS33185 at 2852.249 kb on + strand, within RR42_RS33185 at 2852.249 kb on + strand, within RR42_RS33185 at 2852.250 kb on - strand, within RR42_RS33185 at 2852.250 kb on - strand, within RR42_RS33185 at 2852.250 kb on - strand, within RR42_RS33185 at 2852.250 kb on - strand, within RR42_RS33185 at 2852.250 kb on - strand, within RR42_RS33185 at 2852.251 kb on + strand, within RR42_RS33185 at 2852.251 kb on + strand, within RR42_RS33185 at 2852.345 kb on - strand, within RR42_RS33185 at 2852.345 kb on - strand, within RR42_RS33185 at 2852.412 kb on - strand at 2852.560 kb on + strand at 2852.560 kb on + strand at 2852.561 kb on - strand at 2852.563 kb on + strand at 2852.563 kb on + strand at 2852.563 kb on + strand at 2852.598 kb on + strand at 2852.599 kb on - strand at 2852.599 kb on - strand at 2852.634 kb on - strand at 2852.634 kb on - strand at 2852.645 kb on + strand at 2852.702 kb on + strand, within RR42_RS33190 at 2852.702 kb on + strand, within RR42_RS33190 at 2852.702 kb on + strand, within RR42_RS33190 at 2852.702 kb on + strand, within RR42_RS33190 at 2852.703 kb on - strand, within RR42_RS33190 at 2852.703 kb on - strand, within RR42_RS33190 at 2852.703 kb on - strand, within RR42_RS33190 at 2852.703 kb on - strand, within RR42_RS33190 at 2852.703 kb on - strand, within RR42_RS33190 at 2852.703 kb on - strand, within RR42_RS33190 at 2852.714 kb on + strand, within RR42_RS33190 at 2852.714 kb on + strand, within RR42_RS33190 at 2852.714 kb on + strand, within RR42_RS33190 at 2852.714 kb on + strand, within RR42_RS33190 at 2852.715 kb on - strand, within RR42_RS33190 at 2852.763 kb on + strand, within RR42_RS33190 at 2852.763 kb on + strand, within RR42_RS33190 at 2852.763 kb on + strand, within RR42_RS33190 at 2852.764 kb on - strand, within RR42_RS33190 at 2852.764 kb on - strand, within RR42_RS33190 at 2852.764 kb on - strand, within RR42_RS33190 at 2852.777 kb on + strand, within RR42_RS33190 at 2852.777 kb on + strand, within RR42_RS33190 at 2852.777 kb on + strand, within RR42_RS33190 at 2852.778 kb on - strand, within RR42_RS33190 at 2852.778 kb on - strand, within RR42_RS33190 at 2852.778 kb on - strand, within RR42_RS33190 at 2852.812 kb on + strand, within RR42_RS33190 at 2852.813 kb on - strand, within RR42_RS33190 at 2852.920 kb on + strand, within RR42_RS33190 at 2852.920 kb on + strand, within RR42_RS33190 at 2852.920 kb on + strand, within RR42_RS33190 at 2852.921 kb on - strand, within RR42_RS33190 at 2853.046 kb on + strand, within RR42_RS33190 at 2853.046 kb on + strand, within RR42_RS33190 at 2853.047 kb on - strand, within RR42_RS33190 at 2853.047 kb on - strand, within RR42_RS33190 at 2853.058 kb on + strand, within RR42_RS33190 at 2853.058 kb on + strand, within RR42_RS33190 at 2853.059 kb on - strand, within RR42_RS33190 at 2853.201 kb on + strand, within RR42_RS33190 at 2853.310 kb on + strand at 2853.310 kb on + strand at 2853.310 kb on + strand at 2853.310 kb on + strand at 2853.311 kb on - strand at 2853.311 kb on - strand at 2853.311 kb on - strand at 2853.311 kb on - strand at 2853.311 kb on - strand at 2853.322 kb on + strand at 2853.322 kb on + strand at 2853.322 kb on + strand at 2853.322 kb on + strand at 2853.322 kb on + strand at 2853.323 kb on - strand at 2853.323 kb on - strand at 2853.323 kb on - strand at 2853.323 kb on - strand at 2853.323 kb on - strand at 2853.369 kb on + strand at 2853.381 kb on - strand at 2853.381 kb on - strand at 2853.387 kb on + strand at 2853.387 kb on + strand at 2853.387 kb on + strand at 2853.388 kb on - strand at 2853.388 kb on - strand at 2853.388 kb on - strand at 2853.389 kb on + strand at 2853.398 kb on + strand at 2853.398 kb on + strand at 2853.399 kb on - strand at 2853.399 kb on - strand at 2853.399 kb on - strand at 2853.425 kb on + strand at 2853.425 kb on + strand at 2853.425 kb on + strand at 2853.426 kb on - strand at 2853.426 kb on - strand at 2853.451 kb on - strand at 2853.451 kb on - strand at 2853.488 kb on + strand at 2853.488 kb on + strand at 2853.489 kb on - strand at 2853.489 kb on - strand at 2853.489 kb on - strand at 2853.489 kb on - strand at 2853.494 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,850,597 + RR42_RS33180 0.82 +2.7 2,850,672 - +0.6 2,850,679 + +1.6 2,850,680 - +1.6 2,850,691 + +2.6 2,850,691 + +1.9 2,850,691 + +2.7 2,850,692 - +0.8 2,850,692 - +2.6 2,850,732 + +2.1 2,850,732 + +2.8 2,850,733 - +0.9 2,850,733 - +1.4 2,850,733 - +0.4 2,850,747 - +2.0 2,850,747 - +1.3 2,850,762 + +2.9 2,850,763 - +1.0 2,850,763 - +0.1 2,850,763 - +0.3 2,850,763 - -1.2 2,850,787 + +2.6 2,850,791 + +2.5 2,850,791 + +2.9 2,850,791 + +2.8 2,850,791 + +0.6 2,850,791 + +2.1 2,850,792 - +0.5 2,850,795 + +1.6 2,850,795 + +1.0 2,850,795 + +1.9 2,850,796 - +0.9 2,850,796 - +2.1 2,850,796 - +0.0 2,850,796 - -0.2 2,850,796 - +0.5 2,850,797 + +0.8 2,850,797 + +2.4 2,850,798 - +0.2 2,850,844 + +1.5 2,850,847 + +2.3 2,850,847 + +1.0 2,850,847 + +2.0 2,850,848 - +2.1 2,850,848 - +0.7 2,850,848 - +0.3 2,850,938 + +2.2 2,850,974 + +1.7 2,850,974 + +3.1 2,850,974 + +1.9 2,850,974 + +2.3 2,851,023 + +3.2 2,851,025 + +3.2 2,851,027 + +1.5 2,851,027 + +2.1 2,851,027 + +3.3 2,851,027 + +2.1 2,851,027 + +2.3 2,851,028 - -0.2 2,851,028 - +1.9 2,851,028 - +0.6 2,851,028 - +1.1 2,851,028 - -0.2 2,851,028 - +1.1 2,851,028 - +1.6 2,851,028 - +0.6 2,851,029 + +2.1 2,851,029 + +1.8 2,851,029 + +1.6 2,851,029 + +2.3 2,851,030 - -1.0 2,851,030 - +1.8 2,851,030 - +0.4 2,851,180 + +3.5 2,851,233 + +0.8 2,851,355 - +0.3 2,851,355 - -0.7 2,851,355 - -0.3 2,851,430 - +1.6 2,851,475 - -0.6 2,851,488 + +0.2 2,851,620 - -1.1 2,851,715 + RR42_RS33185 0.16 -1.7 2,851,715 + RR42_RS33185 0.16 +0.3 2,851,716 - RR42_RS33185 0.17 -0.4 2,851,716 - RR42_RS33185 0.17 +0.1 2,852,212 - RR42_RS33185 0.69 +0.2 2,852,212 - RR42_RS33185 0.69 +0.4 2,852,213 + RR42_RS33185 0.69 +2.0 2,852,249 + RR42_RS33185 0.73 +0.8 2,852,249 + RR42_RS33185 0.73 -0.2 2,852,249 + RR42_RS33185 0.73 +1.0 2,852,249 + RR42_RS33185 0.73 -0.1 2,852,249 + RR42_RS33185 0.73 -0.1 2,852,250 - RR42_RS33185 0.73 -0.2 2,852,250 - RR42_RS33185 0.73 +0.3 2,852,250 - RR42_RS33185 0.73 +0.3 2,852,250 - RR42_RS33185 0.73 -0.3 2,852,250 - RR42_RS33185 0.73 +0.0 2,852,251 + RR42_RS33185 0.73 -1.4 2,852,251 + RR42_RS33185 0.73 +0.8 2,852,345 - RR42_RS33185 0.83 -2.2 2,852,345 - RR42_RS33185 0.83 +0.0 2,852,412 - -0.3 2,852,560 + +0.6 2,852,560 + +0.5 2,852,561 - -0.6 2,852,563 + -0.4 2,852,563 + -1.6 2,852,563 + -0.5 2,852,598 + +1.3 2,852,599 - -0.5 2,852,599 - +0.6 2,852,634 - -0.5 2,852,634 - -0.1 2,852,645 + -1.7 2,852,702 + RR42_RS33190 0.14 +0.2 2,852,702 + RR42_RS33190 0.14 +1.1 2,852,702 + RR42_RS33190 0.14 +0.1 2,852,702 + RR42_RS33190 0.14 +0.1 2,852,703 - RR42_RS33190 0.14 -0.7 2,852,703 - RR42_RS33190 0.14 -0.5 2,852,703 - RR42_RS33190 0.14 -1.0 2,852,703 - RR42_RS33190 0.14 -0.2 2,852,703 - RR42_RS33190 0.14 -0.9 2,852,703 - RR42_RS33190 0.14 +0.0 2,852,714 + RR42_RS33190 0.15 +0.1 2,852,714 + RR42_RS33190 0.15 +0.7 2,852,714 + RR42_RS33190 0.15 +0.5 2,852,714 + RR42_RS33190 0.15 -0.9 2,852,715 - RR42_RS33190 0.16 +0.3 2,852,763 + RR42_RS33190 0.22 -0.1 2,852,763 + RR42_RS33190 0.22 -1.3 2,852,763 + RR42_RS33190 0.22 +0.3 2,852,764 - RR42_RS33190 0.22 -0.6 2,852,764 - RR42_RS33190 0.22 -0.1 2,852,764 - RR42_RS33190 0.22 -2.0 2,852,777 + RR42_RS33190 0.24 -1.9 2,852,777 + RR42_RS33190 0.24 -0.2 2,852,777 + RR42_RS33190 0.24 -2.4 2,852,778 - RR42_RS33190 0.24 +0.5 2,852,778 - RR42_RS33190 0.24 -0.2 2,852,778 - RR42_RS33190 0.24 +0.3 2,852,812 + RR42_RS33190 0.29 -0.3 2,852,813 - RR42_RS33190 0.29 +0.0 2,852,920 + RR42_RS33190 0.43 +0.7 2,852,920 + RR42_RS33190 0.43 +0.0 2,852,920 + RR42_RS33190 0.43 +1.6 2,852,921 - RR42_RS33190 0.43 -0.1 2,853,046 + RR42_RS33190 0.60 +0.3 2,853,046 + RR42_RS33190 0.60 +0.6 2,853,047 - RR42_RS33190 0.60 -1.0 2,853,047 - RR42_RS33190 0.60 +5.6 2,853,058 + RR42_RS33190 0.61 +1.0 2,853,058 + RR42_RS33190 0.61 +0.1 2,853,059 - RR42_RS33190 0.61 -1.0 2,853,201 + RR42_RS33190 0.80 -0.1 2,853,310 + +0.9 2,853,310 + +1.1 2,853,310 + +0.7 2,853,310 + -0.5 2,853,311 - +3.6 2,853,311 - +1.4 2,853,311 - -0.9 2,853,311 - -0.3 2,853,311 - -0.7 2,853,322 + +0.1 2,853,322 + -0.5 2,853,322 + +0.8 2,853,322 + -2.2 2,853,322 + +0.3 2,853,323 - -0.3 2,853,323 - +0.1 2,853,323 - +0.2 2,853,323 - -0.4 2,853,323 - +1.0 2,853,369 + -0.1 2,853,381 - -0.5 2,853,381 - -0.5 2,853,387 + -1.7 2,853,387 + -0.1 2,853,387 + +0.9 2,853,388 - +0.5 2,853,388 - -1.2 2,853,388 - -0.2 2,853,389 + -0.9 2,853,398 + -0.6 2,853,398 + +0.1 2,853,399 - +0.0 2,853,399 - +1.3 2,853,399 - +1.9 2,853,425 + -0.2 2,853,425 + -0.3 2,853,425 + +0.8 2,853,426 - +0.5 2,853,426 - -1.5 2,853,451 - +0.3 2,853,451 - -1.1 2,853,488 + -0.4 2,853,488 + -1.0 2,853,489 - +0.0 2,853,489 - +1.6 2,853,489 - +0.8 2,853,489 - +1.2 2,853,494 + -1.1
Or see this region's nucleotide sequence