Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32805 and RR42_RS32810 are separated by 335 nucleotides RR42_RS32810 and RR42_RS32815 are separated by 216 nucleotides RR42_RS32815 and RR42_RS32820 are separated by 55 nucleotides
RR42_RS32805: RR42_RS32805 - damage-inducible protein, at 2,756,616 to 2,757,512
_RS32805
RR42_RS32810: RR42_RS32810 - hypothetical protein, at 2,757,848 to 2,758,201
_RS32810
RR42_RS32815: RR42_RS32815 - hypothetical protein, at 2,758,418 to 2,759,038
_RS32815
RR42_RS32820: RR42_RS32820 - hypothetical protein, at 2,759,094 to 2,759,534
_RS32820
Position (kb)
2757
2758
2759 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2757.112 kb on + strand, within RR42_RS32805 at 2757.113 kb on - strand, within RR42_RS32805 at 2757.113 kb on - strand, within RR42_RS32805 at 2757.490 kb on - strand at 2757.534 kb on + strand at 2757.610 kb on - strand at 2757.643 kb on + strand at 2757.656 kb on + strand at 2757.656 kb on + strand at 2757.657 kb on - strand at 2757.657 kb on - strand at 2757.662 kb on + strand at 2757.662 kb on + strand at 2757.662 kb on + strand at 2757.663 kb on - strand at 2757.663 kb on - strand at 2757.996 kb on + strand, within RR42_RS32810 at 2757.996 kb on + strand, within RR42_RS32810 at 2757.997 kb on - strand, within RR42_RS32810 at 2758.139 kb on + strand, within RR42_RS32810 at 2758.139 kb on + strand, within RR42_RS32810 at 2758.140 kb on - strand, within RR42_RS32810 at 2758.140 kb on - strand, within RR42_RS32810 at 2758.140 kb on - strand, within RR42_RS32810 at 2758.140 kb on - strand, within RR42_RS32810 at 2758.201 kb on + strand at 2758.213 kb on + strand at 2758.317 kb on + strand at 2758.362 kb on - strand at 2758.395 kb on - strand at 2758.395 kb on - strand at 2758.402 kb on + strand at 2758.402 kb on + strand at 2758.419 kb on + strand at 2758.511 kb on - strand, within RR42_RS32815 at 2758.511 kb on - strand, within RR42_RS32815 at 2758.511 kb on - strand, within RR42_RS32815 at 2758.511 kb on - strand, within RR42_RS32815 at 2758.683 kb on - strand, within RR42_RS32815 at 2758.708 kb on + strand, within RR42_RS32815 at 2758.715 kb on - strand, within RR42_RS32815 at 2758.846 kb on - strand, within RR42_RS32815 at 2758.972 kb on + strand, within RR42_RS32815 at 2758.972 kb on + strand, within RR42_RS32815 at 2758.972 kb on + strand, within RR42_RS32815 at 2758.975 kb on + strand, within RR42_RS32815 at 2758.976 kb on - strand, within RR42_RS32815 at 2759.051 kb on + strand at 2759.052 kb on - strand at 2759.175 kb on + strand, within RR42_RS32820 at 2759.175 kb on + strand, within RR42_RS32820 at 2759.176 kb on - strand, within RR42_RS32820 at 2759.176 kb on - strand, within RR42_RS32820 at 2759.176 kb on - strand, within RR42_RS32820
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,757,112 + RR42_RS32805 0.55 +0.2 2,757,113 - RR42_RS32805 0.55 +2.6 2,757,113 - RR42_RS32805 0.55 -0.6 2,757,490 - +1.5 2,757,534 + +1.6 2,757,610 - -0.4 2,757,643 + -0.5 2,757,656 + -0.1 2,757,656 + +0.9 2,757,657 - +0.3 2,757,657 - -0.6 2,757,662 + -0.1 2,757,662 + +0.5 2,757,662 + +0.0 2,757,663 - -0.3 2,757,663 - +2.6 2,757,996 + RR42_RS32810 0.42 -1.0 2,757,996 + RR42_RS32810 0.42 -0.1 2,757,997 - RR42_RS32810 0.42 -1.4 2,758,139 + RR42_RS32810 0.82 +0.7 2,758,139 + RR42_RS32810 0.82 -0.6 2,758,140 - RR42_RS32810 0.82 -0.1 2,758,140 - RR42_RS32810 0.82 -0.4 2,758,140 - RR42_RS32810 0.82 +0.3 2,758,140 - RR42_RS32810 0.82 -0.6 2,758,201 + +0.5 2,758,213 + +1.0 2,758,317 + -0.8 2,758,362 - -1.9 2,758,395 - -1.3 2,758,395 - -0.1 2,758,402 + +0.4 2,758,402 + -1.5 2,758,419 + -0.2 2,758,511 - RR42_RS32815 0.15 +0.1 2,758,511 - RR42_RS32815 0.15 +0.3 2,758,511 - RR42_RS32815 0.15 -0.7 2,758,511 - RR42_RS32815 0.15 +1.0 2,758,683 - RR42_RS32815 0.43 -0.1 2,758,708 + RR42_RS32815 0.47 -0.9 2,758,715 - RR42_RS32815 0.48 -1.3 2,758,846 - RR42_RS32815 0.69 +0.8 2,758,972 + RR42_RS32815 0.89 +0.6 2,758,972 + RR42_RS32815 0.89 +0.0 2,758,972 + RR42_RS32815 0.89 +2.0 2,758,975 + RR42_RS32815 0.90 +0.0 2,758,976 - RR42_RS32815 0.90 -1.8 2,759,051 + +0.0 2,759,052 - +1.0 2,759,175 + RR42_RS32820 0.18 +0.9 2,759,175 + RR42_RS32820 0.18 -0.7 2,759,176 - RR42_RS32820 0.19 +0.1 2,759,176 - RR42_RS32820 0.19 -0.7 2,759,176 - RR42_RS32820 0.19 +0.5
Or see this region's nucleotide sequence