Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32700 and RR42_RS32705 are separated by 81 nucleotides RR42_RS32705 and RR42_RS32710 are separated by 129 nucleotides RR42_RS32710 and RR42_RS32715 are separated by 162 nucleotides
RR42_RS32700: RR42_RS32700 - alpha/beta hydrolase, at 2,731,320 to 2,732,282
_RS32700
RR42_RS32705: RR42_RS32705 - CoA-transferase, at 2,732,364 to 2,733,581
_RS32705
RR42_RS32710: RR42_RS32710 - hypothetical protein, at 2,733,711 to 2,733,995
_RS32710
RR42_RS32715: RR42_RS32715 - peroxidase, at 2,734,158 to 2,734,811
_RS32715
Position (kb)
2732
2733
2734 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2731.461 kb on + strand, within RR42_RS32700 at 2731.461 kb on + strand, within RR42_RS32700 at 2731.462 kb on - strand, within RR42_RS32700 at 2731.462 kb on - strand, within RR42_RS32700 at 2731.641 kb on - strand, within RR42_RS32700 at 2731.672 kb on - strand, within RR42_RS32700 at 2732.050 kb on - strand, within RR42_RS32700 at 2732.055 kb on + strand, within RR42_RS32700 at 2732.258 kb on + strand at 2732.259 kb on - strand at 2732.280 kb on + strand at 2732.393 kb on - strand at 2732.393 kb on - strand at 2732.463 kb on + strand at 2732.464 kb on - strand at 2732.464 kb on - strand at 2732.559 kb on + strand, within RR42_RS32705 at 2732.601 kb on - strand, within RR42_RS32705 at 2732.601 kb on - strand, within RR42_RS32705 at 2732.670 kb on + strand, within RR42_RS32705 at 2732.671 kb on - strand, within RR42_RS32705 at 2732.671 kb on - strand, within RR42_RS32705 at 2732.694 kb on + strand, within RR42_RS32705 at 2732.695 kb on - strand, within RR42_RS32705 at 2732.695 kb on - strand, within RR42_RS32705 at 2732.742 kb on + strand, within RR42_RS32705 at 2732.742 kb on + strand, within RR42_RS32705 at 2732.743 kb on - strand, within RR42_RS32705 at 2732.778 kb on + strand, within RR42_RS32705 at 2732.796 kb on + strand, within RR42_RS32705 at 2732.976 kb on + strand, within RR42_RS32705 at 2733.037 kb on - strand, within RR42_RS32705 at 2733.178 kb on + strand, within RR42_RS32705 at 2733.178 kb on + strand, within RR42_RS32705 at 2733.178 kb on + strand, within RR42_RS32705 at 2733.403 kb on + strand, within RR42_RS32705 at 2733.504 kb on - strand at 2733.579 kb on + strand at 2733.579 kb on + strand at 2733.662 kb on + strand at 2733.663 kb on - strand at 2733.677 kb on + strand at 2733.678 kb on - strand at 2733.685 kb on + strand at 2733.685 kb on + strand at 2733.686 kb on - strand at 2733.686 kb on - strand at 2733.686 kb on - strand at 2733.846 kb on + strand, within RR42_RS32710 at 2733.847 kb on - strand, within RR42_RS32710 at 2734.072 kb on + strand at 2734.073 kb on - strand at 2734.084 kb on + strand at 2734.092 kb on + strand at 2734.093 kb on - strand at 2734.159 kb on + strand at 2734.216 kb on + strand at 2734.217 kb on - strand at 2734.489 kb on + strand, within RR42_RS32715 at 2734.490 kb on - strand, within RR42_RS32715
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,731,461 + RR42_RS32700 0.15 -0.0 2,731,461 + RR42_RS32700 0.15 +2.6 2,731,462 - RR42_RS32700 0.15 -0.4 2,731,462 - RR42_RS32700 0.15 -0.3 2,731,641 - RR42_RS32700 0.33 -0.5 2,731,672 - RR42_RS32700 0.37 +0.0 2,732,050 - RR42_RS32700 0.76 +0.5 2,732,055 + RR42_RS32700 0.76 -0.5 2,732,258 + +2.6 2,732,259 - -0.8 2,732,280 + -1.0 2,732,393 - +1.3 2,732,393 - +0.3 2,732,463 + -0.6 2,732,464 - -0.2 2,732,464 - -0.2 2,732,559 + RR42_RS32705 0.16 -0.9 2,732,601 - RR42_RS32705 0.19 +0.1 2,732,601 - RR42_RS32705 0.19 +1.0 2,732,670 + RR42_RS32705 0.25 -1.0 2,732,671 - RR42_RS32705 0.25 -0.9 2,732,671 - RR42_RS32705 0.25 +0.8 2,732,694 + RR42_RS32705 0.27 -1.4 2,732,695 - RR42_RS32705 0.27 -0.1 2,732,695 - RR42_RS32705 0.27 +2.3 2,732,742 + RR42_RS32705 0.31 -0.9 2,732,742 + RR42_RS32705 0.31 -1.0 2,732,743 - RR42_RS32705 0.31 +0.1 2,732,778 + RR42_RS32705 0.34 -0.1 2,732,796 + RR42_RS32705 0.35 +1.9 2,732,976 + RR42_RS32705 0.50 +0.4 2,733,037 - RR42_RS32705 0.55 -0.1 2,733,178 + RR42_RS32705 0.67 -1.3 2,733,178 + RR42_RS32705 0.67 +0.2 2,733,178 + RR42_RS32705 0.67 -1.4 2,733,403 + RR42_RS32705 0.85 -0.9 2,733,504 - +0.4 2,733,579 + +1.0 2,733,579 + -0.7 2,733,662 + -0.1 2,733,663 - -0.3 2,733,677 + -1.4 2,733,678 - -0.5 2,733,685 + -0.2 2,733,685 + -0.8 2,733,686 - +0.1 2,733,686 - +0.2 2,733,686 - -0.0 2,733,846 + RR42_RS32710 0.47 +0.7 2,733,847 - RR42_RS32710 0.48 -1.0 2,734,072 + -0.1 2,734,073 - +0.8 2,734,084 + -0.7 2,734,092 + +0.7 2,734,093 - -0.3 2,734,159 + +1.2 2,734,216 + -1.9 2,734,217 - -1.1 2,734,489 + RR42_RS32715 0.51 -1.3 2,734,490 - RR42_RS32715 0.51 -0.4
Or see this region's nucleotide sequence