Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32555 and RR42_RS32560 are separated by 99 nucleotides RR42_RS32560 and RR42_RS32565 are separated by 103 nucleotides
RR42_RS32555: RR42_RS32555 - nitrilotriacetate monooxygenase, at 2,699,425 to 2,700,789
_RS32555
RR42_RS32560: RR42_RS32560 - membrane protein, at 2,700,889 to 2,701,983
_RS32560
RR42_RS32565: RR42_RS32565 - MFS transporter, at 2,702,087 to 2,703,424
_RS32565
Position (kb)
2700
2701
2702 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2700.191 kb on - strand, within RR42_RS32555 at 2701.019 kb on + strand, within RR42_RS32560 at 2701.046 kb on + strand, within RR42_RS32560 at 2701.160 kb on + strand, within RR42_RS32560 at 2701.250 kb on + strand, within RR42_RS32560 at 2701.251 kb on - strand, within RR42_RS32560 at 2701.368 kb on - strand, within RR42_RS32560 at 2701.481 kb on + strand, within RR42_RS32560 at 2701.513 kb on - strand, within RR42_RS32560 at 2701.513 kb on - strand, within RR42_RS32560 at 2701.520 kb on + strand, within RR42_RS32560 at 2701.520 kb on + strand, within RR42_RS32560 at 2701.521 kb on - strand, within RR42_RS32560 at 2701.521 kb on - strand, within RR42_RS32560 at 2701.521 kb on - strand, within RR42_RS32560 at 2701.521 kb on - strand, within RR42_RS32560 at 2701.521 kb on - strand, within RR42_RS32560 at 2701.546 kb on - strand, within RR42_RS32560 at 2701.546 kb on - strand, within RR42_RS32560 at 2701.556 kb on + strand, within RR42_RS32560 at 2701.556 kb on + strand, within RR42_RS32560 at 2701.556 kb on + strand, within RR42_RS32560 at 2701.557 kb on - strand, within RR42_RS32560 at 2701.557 kb on - strand, within RR42_RS32560 at 2701.557 kb on - strand, within RR42_RS32560 at 2701.557 kb on - strand, within RR42_RS32560 at 2701.557 kb on - strand, within RR42_RS32560 at 2701.560 kb on + strand, within RR42_RS32560 at 2701.635 kb on - strand, within RR42_RS32560 at 2701.635 kb on - strand, within RR42_RS32560 at 2701.880 kb on + strand at 2701.880 kb on + strand at 2701.968 kb on + strand at 2701.968 kb on + strand at 2701.969 kb on - strand at 2701.969 kb on - strand at 2701.969 kb on - strand at 2701.969 kb on - strand at 2702.004 kb on + strand at 2702.005 kb on - strand at 2702.051 kb on - strand at 2702.062 kb on + strand at 2702.062 kb on + strand at 2702.062 kb on + strand at 2702.062 kb on + strand at 2702.063 kb on - strand at 2702.063 kb on - strand at 2702.063 kb on - strand at 2702.106 kb on - strand at 2702.187 kb on + strand at 2702.187 kb on + strand at 2702.187 kb on + strand at 2702.188 kb on - strand at 2702.190 kb on + strand at 2702.191 kb on - strand at 2702.191 kb on - strand at 2702.191 kb on - strand at 2702.334 kb on + strand, within RR42_RS32565 at 2702.334 kb on + strand, within RR42_RS32565 at 2702.334 kb on + strand, within RR42_RS32565 at 2702.335 kb on - strand, within RR42_RS32565 at 2702.404 kb on + strand, within RR42_RS32565 at 2702.405 kb on - strand, within RR42_RS32565 at 2702.405 kb on - strand, within RR42_RS32565 at 2702.405 kb on - strand, within RR42_RS32565 at 2702.616 kb on + strand, within RR42_RS32565 at 2702.616 kb on + strand, within RR42_RS32565 at 2702.617 kb on - strand, within RR42_RS32565 at 2702.663 kb on - strand, within RR42_RS32565 at 2702.747 kb on - strand, within RR42_RS32565 at 2702.913 kb on + strand, within RR42_RS32565 at 2702.914 kb on - strand, within RR42_RS32565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,700,191 - RR42_RS32555 0.56 -0.5 2,701,019 + RR42_RS32560 0.12 +0.6 2,701,046 + RR42_RS32560 0.14 +0.3 2,701,160 + RR42_RS32560 0.25 -0.1 2,701,250 + RR42_RS32560 0.33 -0.4 2,701,251 - RR42_RS32560 0.33 -0.7 2,701,368 - RR42_RS32560 0.44 -0.6 2,701,481 + RR42_RS32560 0.54 -0.7 2,701,513 - RR42_RS32560 0.57 +2.0 2,701,513 - RR42_RS32560 0.57 -0.5 2,701,520 + RR42_RS32560 0.58 +0.3 2,701,520 + RR42_RS32560 0.58 +3.9 2,701,521 - RR42_RS32560 0.58 -0.3 2,701,521 - RR42_RS32560 0.58 +0.7 2,701,521 - RR42_RS32560 0.58 +0.8 2,701,521 - RR42_RS32560 0.58 -1.0 2,701,521 - RR42_RS32560 0.58 -0.0 2,701,546 - RR42_RS32560 0.60 +0.6 2,701,546 - RR42_RS32560 0.60 +0.5 2,701,556 + RR42_RS32560 0.61 -0.0 2,701,556 + RR42_RS32560 0.61 +0.9 2,701,556 + RR42_RS32560 0.61 -1.9 2,701,557 - RR42_RS32560 0.61 +1.0 2,701,557 - RR42_RS32560 0.61 +0.8 2,701,557 - RR42_RS32560 0.61 +0.3 2,701,557 - RR42_RS32560 0.61 -0.2 2,701,557 - RR42_RS32560 0.61 +1.4 2,701,560 + RR42_RS32560 0.61 -0.7 2,701,635 - RR42_RS32560 0.68 -0.2 2,701,635 - RR42_RS32560 0.68 +0.5 2,701,880 + -0.3 2,701,880 + +0.4 2,701,968 + +0.1 2,701,968 + -0.4 2,701,969 - +0.7 2,701,969 - -0.6 2,701,969 - -1.9 2,701,969 - +0.7 2,702,004 + -0.3 2,702,005 - +0.8 2,702,051 - -0.5 2,702,062 + +0.1 2,702,062 + -1.3 2,702,062 + -0.5 2,702,062 + -1.5 2,702,063 - -0.3 2,702,063 - +0.6 2,702,063 - -0.0 2,702,106 - -0.3 2,702,187 + +0.1 2,702,187 + +1.6 2,702,187 + -1.4 2,702,188 - +0.1 2,702,190 + -0.1 2,702,191 - -0.2 2,702,191 - -1.2 2,702,191 - -0.4 2,702,334 + RR42_RS32565 0.18 -0.1 2,702,334 + RR42_RS32565 0.18 +0.2 2,702,334 + RR42_RS32565 0.18 +0.4 2,702,335 - RR42_RS32565 0.19 -0.0 2,702,404 + RR42_RS32565 0.24 +0.7 2,702,405 - RR42_RS32565 0.24 +1.0 2,702,405 - RR42_RS32565 0.24 -2.1 2,702,405 - RR42_RS32565 0.24 +0.3 2,702,616 + RR42_RS32565 0.40 -1.1 2,702,616 + RR42_RS32565 0.40 +1.4 2,702,617 - RR42_RS32565 0.40 +0.6 2,702,663 - RR42_RS32565 0.43 +0.4 2,702,747 - RR42_RS32565 0.49 +2.1 2,702,913 + RR42_RS32565 0.62 -0.6 2,702,914 - RR42_RS32565 0.62 +0.6
Or see this region's nucleotide sequence