Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32490 and RR42_RS32495 are separated by 123 nucleotides RR42_RS32495 and RR42_RS32500 are separated by 59 nucleotides RR42_RS32500 and RR42_RS32505 are separated by 76 nucleotides RR42_RS32505 and RR42_RS32510 are separated by 1 nucleotides
RR42_RS32490: RR42_RS32490 - 3-keto-5-aminohexanoate cleavage protein, at 2,687,055 to 2,687,879
_RS32490
RR42_RS32495: RR42_RS32495 - shikimate kinase, at 2,688,003 to 2,688,515
_RS32495
RR42_RS32500: RR42_RS32500 - ABC transporter permease, at 2,688,575 to 2,689,426
_RS32500
RR42_RS32505: RR42_RS32505 - sulfonate ABC transporter ATP-binding protein, at 2,689,503 to 2,690,351
_RS32505
RR42_RS32510: RR42_RS32510 - nitrate ABC transporter substrate-binding protein, at 2,690,353 to 2,691,387
_RS32510
Position (kb)
2688
2689
2690 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.639 kb on - strand, within RR42_RS32490 at 2687.886 kb on + strand at 2687.927 kb on + strand at 2688.270 kb on + strand, within RR42_RS32495 at 2688.271 kb on - strand, within RR42_RS32495 at 2688.693 kb on + strand, within RR42_RS32500 at 2688.721 kb on - strand, within RR42_RS32500 at 2688.721 kb on - strand, within RR42_RS32500 at 2688.721 kb on - strand, within RR42_RS32500 at 2688.721 kb on - strand, within RR42_RS32500 at 2688.721 kb on - strand, within RR42_RS32500 at 2688.861 kb on + strand, within RR42_RS32500 at 2688.861 kb on + strand, within RR42_RS32500 at 2688.862 kb on - strand, within RR42_RS32500 at 2688.862 kb on - strand, within RR42_RS32500 at 2688.862 kb on - strand, within RR42_RS32500 at 2688.883 kb on - strand, within RR42_RS32500 at 2688.933 kb on + strand, within RR42_RS32500 at 2688.933 kb on + strand, within RR42_RS32500 at 2688.933 kb on + strand, within RR42_RS32500 at 2688.933 kb on + strand, within RR42_RS32500 at 2688.934 kb on - strand, within RR42_RS32500 at 2688.934 kb on - strand, within RR42_RS32500 at 2689.146 kb on + strand, within RR42_RS32500 at 2689.410 kb on + strand at 2689.443 kb on + strand at 2690.263 kb on + strand, within RR42_RS32505 at 2690.264 kb on - strand, within RR42_RS32505 at 2690.390 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,687,638 + RR42_RS32490 0.71 +2.3 2,687,638 + RR42_RS32490 0.71 +0.3 2,687,638 + RR42_RS32490 0.71 -0.1 2,687,638 + RR42_RS32490 0.71 -1.9 2,687,638 + RR42_RS32490 0.71 +0.3 2,687,638 + RR42_RS32490 0.71 +0.1 2,687,638 + RR42_RS32490 0.71 +0.2 2,687,638 + RR42_RS32490 0.71 +0.7 2,687,639 - RR42_RS32490 0.71 -0.1 2,687,886 + +0.1 2,687,927 + +0.0 2,688,270 + RR42_RS32495 0.52 +1.2 2,688,271 - RR42_RS32495 0.52 -1.0 2,688,693 + RR42_RS32500 0.14 +0.9 2,688,721 - RR42_RS32500 0.17 +1.0 2,688,721 - RR42_RS32500 0.17 -1.1 2,688,721 - RR42_RS32500 0.17 +0.2 2,688,721 - RR42_RS32500 0.17 +1.1 2,688,721 - RR42_RS32500 0.17 +0.6 2,688,861 + RR42_RS32500 0.34 -1.7 2,688,861 + RR42_RS32500 0.34 +0.4 2,688,862 - RR42_RS32500 0.34 -0.7 2,688,862 - RR42_RS32500 0.34 +1.6 2,688,862 - RR42_RS32500 0.34 +0.1 2,688,883 - RR42_RS32500 0.36 -0.4 2,688,933 + RR42_RS32500 0.42 -0.2 2,688,933 + RR42_RS32500 0.42 -1.7 2,688,933 + RR42_RS32500 0.42 +0.8 2,688,933 + RR42_RS32500 0.42 +0.3 2,688,934 - RR42_RS32500 0.42 +0.8 2,688,934 - RR42_RS32500 0.42 -0.0 2,689,146 + RR42_RS32500 0.67 -1.1 2,689,410 + +0.3 2,689,443 + -1.7 2,690,263 + RR42_RS32505 0.90 +0.6 2,690,264 - RR42_RS32505 0.90 -0.3 2,690,390 + -0.4
Or see this region's nucleotide sequence