Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32485 and RR42_RS32490 are separated by 46 nucleotides RR42_RS32490 and RR42_RS32495 are separated by 123 nucleotides RR42_RS32495 and RR42_RS32500 are separated by 59 nucleotides
RR42_RS32485: RR42_RS32485 - 3-keto-5-aminohexanoate cleavage protein, at 2,685,125 to 2,687,008
_RS32485
RR42_RS32490: RR42_RS32490 - 3-keto-5-aminohexanoate cleavage protein, at 2,687,055 to 2,687,879
_RS32490
RR42_RS32495: RR42_RS32495 - shikimate kinase, at 2,688,003 to 2,688,515
_RS32495
RR42_RS32500: RR42_RS32500 - ABC transporter permease, at 2,688,575 to 2,689,426
_RS32500
Position (kb)
2687
2688 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2686.331 kb on + strand, within RR42_RS32485 at 2686.368 kb on - strand, within RR42_RS32485 at 2686.487 kb on + strand, within RR42_RS32485 at 2686.487 kb on + strand, within RR42_RS32485 at 2686.488 kb on - strand, within RR42_RS32485 at 2686.542 kb on - strand, within RR42_RS32485 at 2686.757 kb on + strand, within RR42_RS32485 at 2686.757 kb on + strand, within RR42_RS32485 at 2686.758 kb on - strand, within RR42_RS32485 at 2686.758 kb on - strand, within RR42_RS32485 at 2686.758 kb on - strand, within RR42_RS32485 at 2686.758 kb on - strand, within RR42_RS32485 at 2686.758 kb on - strand, within RR42_RS32485 at 2686.760 kb on + strand, within RR42_RS32485 at 2686.760 kb on + strand, within RR42_RS32485 at 2686.761 kb on - strand, within RR42_RS32485 at 2686.830 kb on + strand at 2686.831 kb on - strand at 2686.868 kb on + strand at 2686.868 kb on + strand at 2686.869 kb on - strand at 2686.869 kb on - strand at 2686.923 kb on - strand at 2687.460 kb on + strand, within RR42_RS32490 at 2687.460 kb on + strand, within RR42_RS32490 at 2687.461 kb on - strand, within RR42_RS32490 at 2687.505 kb on + strand, within RR42_RS32490 at 2687.506 kb on - strand, within RR42_RS32490 at 2687.506 kb on - strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.638 kb on + strand, within RR42_RS32490 at 2687.639 kb on - strand, within RR42_RS32490 at 2687.886 kb on + strand at 2687.927 kb on + strand at 2688.270 kb on + strand, within RR42_RS32495 at 2688.271 kb on - strand, within RR42_RS32495 at 2688.693 kb on + strand, within RR42_RS32500 at 2688.721 kb on - strand, within RR42_RS32500 at 2688.721 kb on - strand, within RR42_RS32500 at 2688.721 kb on - strand, within RR42_RS32500 at 2688.721 kb on - strand, within RR42_RS32500 at 2688.721 kb on - strand, within RR42_RS32500 at 2688.861 kb on + strand, within RR42_RS32500 at 2688.861 kb on + strand, within RR42_RS32500 at 2688.862 kb on - strand, within RR42_RS32500 at 2688.862 kb on - strand, within RR42_RS32500 at 2688.862 kb on - strand, within RR42_RS32500
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,686,331 + RR42_RS32485 0.64 +0.7 2,686,368 - RR42_RS32485 0.66 -0.6 2,686,487 + RR42_RS32485 0.72 -1.0 2,686,487 + RR42_RS32485 0.72 +0.9 2,686,488 - RR42_RS32485 0.72 -0.2 2,686,542 - RR42_RS32485 0.75 -0.8 2,686,757 + RR42_RS32485 0.87 -1.0 2,686,757 + RR42_RS32485 0.87 +0.0 2,686,758 - RR42_RS32485 0.87 +0.3 2,686,758 - RR42_RS32485 0.87 +0.6 2,686,758 - RR42_RS32485 0.87 +0.4 2,686,758 - RR42_RS32485 0.87 +0.4 2,686,758 - RR42_RS32485 0.87 +0.1 2,686,760 + RR42_RS32485 0.87 -0.6 2,686,760 + RR42_RS32485 0.87 +1.6 2,686,761 - RR42_RS32485 0.87 +0.4 2,686,830 + -0.2 2,686,831 - +0.8 2,686,868 + -1.1 2,686,868 + +1.6 2,686,869 - -1.0 2,686,869 - -1.2 2,686,923 - -0.7 2,687,460 + RR42_RS32490 0.49 +0.9 2,687,460 + RR42_RS32490 0.49 +0.6 2,687,461 - RR42_RS32490 0.49 -0.2 2,687,505 + RR42_RS32490 0.55 -0.3 2,687,506 - RR42_RS32490 0.55 -0.6 2,687,506 - RR42_RS32490 0.55 -0.9 2,687,638 + RR42_RS32490 0.71 +2.3 2,687,638 + RR42_RS32490 0.71 +0.3 2,687,638 + RR42_RS32490 0.71 -0.1 2,687,638 + RR42_RS32490 0.71 -1.9 2,687,638 + RR42_RS32490 0.71 +0.3 2,687,638 + RR42_RS32490 0.71 +0.1 2,687,638 + RR42_RS32490 0.71 +0.2 2,687,638 + RR42_RS32490 0.71 +0.7 2,687,639 - RR42_RS32490 0.71 -0.1 2,687,886 + +0.1 2,687,927 + +0.0 2,688,270 + RR42_RS32495 0.52 +1.2 2,688,271 - RR42_RS32495 0.52 -1.0 2,688,693 + RR42_RS32500 0.14 +0.9 2,688,721 - RR42_RS32500 0.17 +1.0 2,688,721 - RR42_RS32500 0.17 -1.1 2,688,721 - RR42_RS32500 0.17 +0.2 2,688,721 - RR42_RS32500 0.17 +1.1 2,688,721 - RR42_RS32500 0.17 +0.6 2,688,861 + RR42_RS32500 0.34 -1.7 2,688,861 + RR42_RS32500 0.34 +0.4 2,688,862 - RR42_RS32500 0.34 -0.7 2,688,862 - RR42_RS32500 0.34 +1.6 2,688,862 - RR42_RS32500 0.34 +0.1
Or see this region's nucleotide sequence