Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32225 and RR42_RS32230 are separated by 158 nucleotides RR42_RS32230 and RR42_RS32235 are separated by 33 nucleotides
RR42_RS32225: RR42_RS32225 - NAD-dependent dehydratase, at 2,616,235 to 2,617,236
_RS32225
RR42_RS32230: RR42_RS32230 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 2,617,395 to 2,618,396
_RS32230
RR42_RS32235: RR42_RS32235 - hopanoid biosynthesis associated radical SAM protein HpnH, at 2,618,430 to 2,619,596
_RS32235
Position (kb)
2617
2618
2619 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.546 kb on - strand, within RR42_RS32225 at 2616.546 kb on - strand, within RR42_RS32225 at 2616.546 kb on - strand, within RR42_RS32225 at 2616.583 kb on - strand, within RR42_RS32225 at 2616.583 kb on - strand, within RR42_RS32225 at 2616.583 kb on - strand, within RR42_RS32225 at 2616.583 kb on - strand, within RR42_RS32225 at 2616.583 kb on - strand, within RR42_RS32225 at 2616.664 kb on + strand, within RR42_RS32225 at 2616.664 kb on + strand, within RR42_RS32225 at 2616.665 kb on - strand, within RR42_RS32225 at 2616.804 kb on - strand, within RR42_RS32225 at 2616.896 kb on + strand, within RR42_RS32225 at 2616.897 kb on - strand, within RR42_RS32225 at 2616.897 kb on - strand, within RR42_RS32225 at 2616.897 kb on - strand, within RR42_RS32225 at 2617.002 kb on - strand, within RR42_RS32225 at 2617.025 kb on + strand, within RR42_RS32225 at 2617.025 kb on + strand, within RR42_RS32225 at 2617.025 kb on + strand, within RR42_RS32225 at 2617.026 kb on - strand, within RR42_RS32225 at 2617.026 kb on - strand, within RR42_RS32225 at 2617.220 kb on + strand at 2617.236 kb on + strand at 2617.237 kb on - strand at 2617.237 kb on - strand at 2617.308 kb on + strand at 2617.308 kb on + strand at 2617.309 kb on - strand at 2617.310 kb on + strand at 2617.310 kb on + strand at 2617.310 kb on + strand at 2617.310 kb on + strand at 2617.312 kb on + strand at 2617.312 kb on + strand at 2617.312 kb on + strand at 2617.312 kb on + strand at 2617.312 kb on + strand at 2617.312 kb on + strand at 2617.312 kb on + strand at 2617.313 kb on - strand at 2617.313 kb on - strand at 2617.313 kb on - strand at 2617.313 kb on - strand at 2617.315 kb on + strand at 2617.315 kb on + strand at 2617.316 kb on - strand at 2617.348 kb on + strand at 2617.356 kb on + strand at 2617.357 kb on - strand at 2617.357 kb on - strand at 2617.388 kb on + strand at 2617.388 kb on + strand at 2617.389 kb on - strand at 2617.389 kb on - strand at 2617.394 kb on + strand at 2617.395 kb on - strand at 2617.459 kb on + strand at 2617.460 kb on - strand at 2617.460 kb on - strand at 2617.719 kb on + strand, within RR42_RS32230 at 2617.720 kb on - strand, within RR42_RS32230 at 2617.873 kb on - strand, within RR42_RS32230 at 2617.887 kb on - strand, within RR42_RS32230 at 2617.887 kb on - strand, within RR42_RS32230 at 2617.887 kb on - strand, within RR42_RS32230 at 2617.910 kb on + strand, within RR42_RS32230 at 2617.911 kb on - strand, within RR42_RS32230 at 2617.911 kb on - strand, within RR42_RS32230 at 2617.911 kb on - strand, within RR42_RS32230 at 2617.911 kb on - strand, within RR42_RS32230 at 2617.913 kb on + strand, within RR42_RS32230 at 2617.913 kb on + strand, within RR42_RS32230 at 2617.914 kb on - strand, within RR42_RS32230 at 2617.914 kb on - strand, within RR42_RS32230 at 2617.915 kb on + strand, within RR42_RS32230 at 2617.915 kb on + strand, within RR42_RS32230 at 2617.915 kb on + strand, within RR42_RS32230 at 2617.916 kb on - strand, within RR42_RS32230 at 2617.916 kb on - strand, within RR42_RS32230 at 2617.943 kb on + strand, within RR42_RS32230 at 2617.977 kb on + strand, within RR42_RS32230 at 2618.058 kb on - strand, within RR42_RS32230 at 2618.058 kb on - strand, within RR42_RS32230 at 2618.112 kb on + strand, within RR42_RS32230 at 2618.413 kb on + strand at 2618.425 kb on - strand at 2618.470 kb on - strand at 2618.470 kb on - strand at 2618.555 kb on + strand, within RR42_RS32235 at 2618.784 kb on + strand, within RR42_RS32235 at 2618.785 kb on - strand, within RR42_RS32235 at 2618.872 kb on - strand, within RR42_RS32235 at 2619.030 kb on + strand, within RR42_RS32235 at 2619.186 kb on + strand, within RR42_RS32235 at 2619.187 kb on - strand, within RR42_RS32235 at 2619.187 kb on - strand, within RR42_RS32235 at 2619.246 kb on + strand, within RR42_RS32235 at 2619.246 kb on + strand, within RR42_RS32235 at 2619.265 kb on - strand, within RR42_RS32235 at 2619.312 kb on + strand, within RR42_RS32235 at 2619.313 kb on - strand, within RR42_RS32235 at 2619.357 kb on - strand, within RR42_RS32235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,616,545 + RR42_RS32225 0.31 -1.9 2,616,545 + RR42_RS32225 0.31 -1.4 2,616,545 + RR42_RS32225 0.31 -0.7 2,616,545 + RR42_RS32225 0.31 +0.7 2,616,545 + RR42_RS32225 0.31 +0.0 2,616,545 + RR42_RS32225 0.31 +0.0 2,616,545 + RR42_RS32225 0.31 +1.0 2,616,545 + RR42_RS32225 0.31 -1.2 2,616,546 - RR42_RS32225 0.31 +0.8 2,616,546 - RR42_RS32225 0.31 -0.6 2,616,546 - RR42_RS32225 0.31 -0.6 2,616,583 - RR42_RS32225 0.35 +1.6 2,616,583 - RR42_RS32225 0.35 -0.5 2,616,583 - RR42_RS32225 0.35 +0.1 2,616,583 - RR42_RS32225 0.35 +1.0 2,616,583 - RR42_RS32225 0.35 -0.0 2,616,664 + RR42_RS32225 0.43 -0.4 2,616,664 + RR42_RS32225 0.43 -1.0 2,616,665 - RR42_RS32225 0.43 -0.6 2,616,804 - RR42_RS32225 0.57 -1.7 2,616,896 + RR42_RS32225 0.66 -0.5 2,616,897 - RR42_RS32225 0.66 -0.1 2,616,897 - RR42_RS32225 0.66 -1.5 2,616,897 - RR42_RS32225 0.66 +1.4 2,617,002 - RR42_RS32225 0.77 +0.0 2,617,025 + RR42_RS32225 0.79 -0.3 2,617,025 + RR42_RS32225 0.79 +0.4 2,617,025 + RR42_RS32225 0.79 +0.0 2,617,026 - RR42_RS32225 0.79 -1.3 2,617,026 - RR42_RS32225 0.79 -0.3 2,617,220 + -0.6 2,617,236 + -0.7 2,617,237 - -0.8 2,617,237 - -2.2 2,617,308 + -1.1 2,617,308 + -0.6 2,617,309 - -2.7 2,617,310 + -2.3 2,617,310 + -0.5 2,617,310 + +1.3 2,617,310 + -0.4 2,617,312 + +0.6 2,617,312 + +0.2 2,617,312 + -0.3 2,617,312 + +0.0 2,617,312 + +1.1 2,617,312 + +0.1 2,617,312 + -1.6 2,617,313 - +3.0 2,617,313 - -0.4 2,617,313 - -0.7 2,617,313 - -0.7 2,617,315 + +0.6 2,617,315 + +0.1 2,617,316 - -0.2 2,617,348 + -1.3 2,617,356 + -1.1 2,617,357 - -0.6 2,617,357 - -0.5 2,617,388 + +0.0 2,617,388 + +0.2 2,617,389 - +1.7 2,617,389 - +0.4 2,617,394 + -0.3 2,617,395 - +0.0 2,617,459 + -1.4 2,617,460 - +0.1 2,617,460 - +0.3 2,617,719 + RR42_RS32230 0.32 +1.2 2,617,720 - RR42_RS32230 0.32 +0.4 2,617,873 - RR42_RS32230 0.48 +1.4 2,617,887 - RR42_RS32230 0.49 -0.8 2,617,887 - RR42_RS32230 0.49 +0.0 2,617,887 - RR42_RS32230 0.49 +0.1 2,617,910 + RR42_RS32230 0.51 +0.3 2,617,911 - RR42_RS32230 0.51 +0.1 2,617,911 - RR42_RS32230 0.51 -1.4 2,617,911 - RR42_RS32230 0.51 -1.9 2,617,911 - RR42_RS32230 0.51 -0.6 2,617,913 + RR42_RS32230 0.52 -1.6 2,617,913 + RR42_RS32230 0.52 -0.4 2,617,914 - RR42_RS32230 0.52 -1.0 2,617,914 - RR42_RS32230 0.52 +0.8 2,617,915 + RR42_RS32230 0.52 -0.7 2,617,915 + RR42_RS32230 0.52 -0.1 2,617,915 + RR42_RS32230 0.52 -0.3 2,617,916 - RR42_RS32230 0.52 -1.4 2,617,916 - RR42_RS32230 0.52 -1.1 2,617,943 + RR42_RS32230 0.55 -1.2 2,617,977 + RR42_RS32230 0.58 -0.3 2,618,058 - RR42_RS32230 0.66 -1.0 2,618,058 - RR42_RS32230 0.66 -1.3 2,618,112 + RR42_RS32230 0.72 +0.4 2,618,413 + +0.6 2,618,425 - +0.0 2,618,470 - -1.4 2,618,470 - -1.2 2,618,555 + RR42_RS32235 0.11 +0.4 2,618,784 + RR42_RS32235 0.30 -1.5 2,618,785 - RR42_RS32235 0.30 +1.3 2,618,872 - RR42_RS32235 0.38 -2.8 2,619,030 + RR42_RS32235 0.51 +1.6 2,619,186 + RR42_RS32235 0.65 -1.6 2,619,187 - RR42_RS32235 0.65 -0.9 2,619,187 - RR42_RS32235 0.65 -0.9 2,619,246 + RR42_RS32235 0.70 -0.8 2,619,246 + RR42_RS32235 0.70 -0.1 2,619,265 - RR42_RS32235 0.72 -2.6 2,619,312 + RR42_RS32235 0.76 +0.2 2,619,313 - RR42_RS32235 0.76 +0.3 2,619,357 - RR42_RS32235 0.79 -0.0
Or see this region's nucleotide sequence