Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32030 and RR42_RS32035 are separated by 440 nucleotides RR42_RS32035 and RR42_RS32040 are separated by 64 nucleotides
RR42_RS32030: RR42_RS32030 - LuxR family transcriptional regulator, at 2,562,667 to 2,563,854
_RS32030
RR42_RS32035: RR42_RS32035 - short-chain dehydrogenase, at 2,564,295 to 2,565,104
_RS32035
RR42_RS32040: RR42_RS32040 - esterase, at 2,565,169 to 2,566,161
_RS32040
Position (kb)
2564
2565
2566 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2563.575 kb on - strand, within RR42_RS32030 at 2563.577 kb on + strand, within RR42_RS32030 at 2563.577 kb on + strand, within RR42_RS32030 at 2563.586 kb on + strand, within RR42_RS32030 at 2563.587 kb on - strand, within RR42_RS32030 at 2563.587 kb on - strand, within RR42_RS32030 at 2563.587 kb on - strand, within RR42_RS32030 at 2563.685 kb on + strand, within RR42_RS32030 at 2563.686 kb on - strand, within RR42_RS32030 at 2563.757 kb on + strand at 2563.854 kb on + strand at 2563.855 kb on - strand at 2563.855 kb on - strand at 2563.855 kb on - strand at 2563.873 kb on - strand at 2563.942 kb on + strand at 2563.944 kb on + strand at 2563.944 kb on + strand at 2563.945 kb on - strand at 2564.092 kb on + strand at 2564.093 kb on - strand at 2564.093 kb on - strand at 2564.104 kb on + strand at 2564.141 kb on + strand at 2564.141 kb on + strand at 2564.233 kb on - strand at 2564.761 kb on - strand, within RR42_RS32035 at 2564.761 kb on - strand, within RR42_RS32035 at 2564.762 kb on + strand, within RR42_RS32035 at 2564.762 kb on + strand, within RR42_RS32035 at 2565.364 kb on + strand, within RR42_RS32040 at 2565.453 kb on + strand, within RR42_RS32040 at 2565.454 kb on - strand, within RR42_RS32040 at 2565.454 kb on - strand, within RR42_RS32040 at 2565.709 kb on + strand, within RR42_RS32040 at 2565.716 kb on - strand, within RR42_RS32040 at 2565.716 kb on - strand, within RR42_RS32040 at 2565.809 kb on - strand, within RR42_RS32040
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,563,575 - RR42_RS32030 0.76 -1.7 2,563,577 + RR42_RS32030 0.77 -0.4 2,563,577 + RR42_RS32030 0.77 +0.3 2,563,586 + RR42_RS32030 0.77 -1.6 2,563,587 - RR42_RS32030 0.77 +1.0 2,563,587 - RR42_RS32030 0.77 +1.6 2,563,587 - RR42_RS32030 0.77 +1.2 2,563,685 + RR42_RS32030 0.86 +0.2 2,563,686 - RR42_RS32030 0.86 -0.1 2,563,757 + -0.1 2,563,854 + +2.2 2,563,855 - -1.2 2,563,855 - +0.4 2,563,855 - +1.6 2,563,873 - +0.9 2,563,942 + -1.0 2,563,944 + +1.1 2,563,944 + +1.1 2,563,945 - +0.0 2,564,092 + +1.0 2,564,093 - +0.3 2,564,093 - +0.3 2,564,104 + -1.5 2,564,141 + +0.0 2,564,141 + +0.1 2,564,233 - +0.9 2,564,761 - RR42_RS32035 0.58 -0.8 2,564,761 - RR42_RS32035 0.58 -1.2 2,564,762 + RR42_RS32035 0.58 +0.2 2,564,762 + RR42_RS32035 0.58 -1.4 2,565,364 + RR42_RS32040 0.20 +0.0 2,565,453 + RR42_RS32040 0.29 +0.5 2,565,454 - RR42_RS32040 0.29 +1.3 2,565,454 - RR42_RS32040 0.29 +0.1 2,565,709 + RR42_RS32040 0.54 -0.3 2,565,716 - RR42_RS32040 0.55 -2.1 2,565,716 - RR42_RS32040 0.55 +0.3 2,565,809 - RR42_RS32040 0.64 -1.3
Or see this region's nucleotide sequence