Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31845 and RR42_RS31850 are separated by 85 nucleotides RR42_RS31850 and RR42_RS31855 are separated by 55 nucleotides RR42_RS31855 and RR42_RS31860 are separated by 10 nucleotides
RR42_RS31845: RR42_RS31845 - hypothetical protein, at 2,521,417 to 2,522,796
_RS31845
RR42_RS31850: RR42_RS31850 - 2-keto-4-pentenoate hydratase, at 2,522,882 to 2,523,664
_RS31850
RR42_RS31855: RR42_RS31855 - acetaldehyde dehydrogenase, at 2,523,720 to 2,524,631
_RS31855
RR42_RS31860: RR42_RS31860 - 4-hyroxy-2-oxovalerate aldolase, at 2,524,642 to 2,525,664
_RS31860
Position (kb)
2522
2523
2524 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2522.035 kb on + strand, within RR42_RS31845 at 2522.159 kb on - strand, within RR42_RS31845 at 2522.206 kb on + strand, within RR42_RS31845 at 2522.206 kb on + strand, within RR42_RS31845 at 2522.206 kb on + strand, within RR42_RS31845 at 2522.207 kb on - strand, within RR42_RS31845 at 2522.360 kb on - strand, within RR42_RS31845 at 2522.368 kb on + strand, within RR42_RS31845 at 2522.377 kb on + strand, within RR42_RS31845 at 2522.378 kb on - strand, within RR42_RS31845 at 2522.378 kb on - strand, within RR42_RS31845 at 2522.530 kb on + strand, within RR42_RS31845 at 2522.531 kb on - strand, within RR42_RS31845 at 2522.531 kb on - strand, within RR42_RS31845 at 2522.626 kb on + strand, within RR42_RS31845 at 2522.758 kb on + strand at 2522.798 kb on - strand at 2523.372 kb on - strand, within RR42_RS31850 at 2523.394 kb on - strand, within RR42_RS31850 at 2523.778 kb on - strand at 2524.097 kb on + strand, within RR42_RS31855 at 2524.097 kb on + strand, within RR42_RS31855 at 2524.097 kb on + strand, within RR42_RS31855 at 2524.098 kb on - strand, within RR42_RS31855 at 2524.137 kb on + strand, within RR42_RS31855 at 2524.138 kb on - strand, within RR42_RS31855 at 2524.145 kb on - strand, within RR42_RS31855 at 2524.426 kb on - strand, within RR42_RS31855 at 2524.426 kb on - strand, within RR42_RS31855 at 2524.426 kb on - strand, within RR42_RS31855 at 2524.426 kb on - strand, within RR42_RS31855 at 2524.438 kb on - strand, within RR42_RS31855 at 2524.439 kb on + strand, within RR42_RS31855 at 2524.440 kb on - strand, within RR42_RS31855 at 2524.513 kb on - strand, within RR42_RS31855 at 2524.524 kb on + strand, within RR42_RS31855 at 2524.525 kb on - strand, within RR42_RS31855 at 2524.638 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,522,035 + RR42_RS31845 0.45 -0.2 2,522,159 - RR42_RS31845 0.54 -1.6 2,522,206 + RR42_RS31845 0.57 +0.3 2,522,206 + RR42_RS31845 0.57 -0.1 2,522,206 + RR42_RS31845 0.57 -0.2 2,522,207 - RR42_RS31845 0.57 -0.0 2,522,360 - RR42_RS31845 0.68 -0.2 2,522,368 + RR42_RS31845 0.69 -1.0 2,522,377 + RR42_RS31845 0.70 +1.3 2,522,378 - RR42_RS31845 0.70 -0.8 2,522,378 - RR42_RS31845 0.70 +2.6 2,522,530 + RR42_RS31845 0.81 +2.0 2,522,531 - RR42_RS31845 0.81 -0.3 2,522,531 - RR42_RS31845 0.81 +1.0 2,522,626 + RR42_RS31845 0.88 +0.5 2,522,758 + -0.2 2,522,798 - +1.2 2,523,372 - RR42_RS31850 0.63 -1.0 2,523,394 - RR42_RS31850 0.65 -1.4 2,523,778 - -1.2 2,524,097 + RR42_RS31855 0.41 -0.3 2,524,097 + RR42_RS31855 0.41 +0.6 2,524,097 + RR42_RS31855 0.41 +0.5 2,524,098 - RR42_RS31855 0.41 -0.6 2,524,137 + RR42_RS31855 0.46 +0.6 2,524,138 - RR42_RS31855 0.46 +1.3 2,524,145 - RR42_RS31855 0.47 +1.2 2,524,426 - RR42_RS31855 0.77 -1.8 2,524,426 - RR42_RS31855 0.77 -0.9 2,524,426 - RR42_RS31855 0.77 +0.8 2,524,426 - RR42_RS31855 0.77 -0.9 2,524,438 - RR42_RS31855 0.79 -0.8 2,524,439 + RR42_RS31855 0.79 -1.4 2,524,440 - RR42_RS31855 0.79 +0.7 2,524,513 - RR42_RS31855 0.87 -1.4 2,524,524 + RR42_RS31855 0.88 +0.0 2,524,525 - RR42_RS31855 0.88 -0.1 2,524,638 - +0.5
Or see this region's nucleotide sequence