Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS31820

Experiment: R2A with Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS31810 and RR42_RS31815 are separated by 18 nucleotidesRR42_RS31815 and RR42_RS31820 are separated by 51 nucleotidesRR42_RS31820 and RR42_RS31825 are separated by 56 nucleotides RR42_RS31810: RR42_RS31810 - hypothetical protein, at 2,514,991 to 2,515,545 _RS31810 RR42_RS31815: RR42_RS31815 - steroid delta-isomerase, at 2,515,564 to 2,515,953 _RS31815 RR42_RS31820: RR42_RS31820 - short-chain dehydrogenase, at 2,516,005 to 2,516,790 _RS31820 RR42_RS31825: RR42_RS31825 - 3-oxoacyl-ACP reductase, at 2,516,847 to 2,517,695 _RS31825 Position (kb) 2516 2517Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 2515.072 kb on + strand, within RR42_RS31810at 2515.401 kb on - strand, within RR42_RS31810at 2515.566 kb on - strandat 2515.686 kb on - strand, within RR42_RS31815at 2515.778 kb on + strand, within RR42_RS31815at 2515.778 kb on + strand, within RR42_RS31815at 2516.079 kb on - strandat 2516.208 kb on + strand, within RR42_RS31820at 2516.209 kb on - strand, within RR42_RS31820at 2516.253 kb on - strand, within RR42_RS31820at 2516.339 kb on + strand, within RR42_RS31820at 2516.784 kb on - strandat 2516.902 kb on + strandat 2516.902 kb on + strandat 2517.212 kb on + strand, within RR42_RS31825at 2517.213 kb on - strand, within RR42_RS31825at 2517.341 kb on - strand, within RR42_RS31825at 2517.554 kb on + strand, within RR42_RS31825at 2517.554 kb on + strand, within RR42_RS31825at 2517.686 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml
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2,515,072 + RR42_RS31810 0.15 -0.7
2,515,401 - RR42_RS31810 0.74 +1.0
2,515,566 - +5.4
2,515,686 - RR42_RS31815 0.31 -1.2
2,515,778 + RR42_RS31815 0.55 +0.3
2,515,778 + RR42_RS31815 0.55 -0.6
2,516,079 - +0.6
2,516,208 + RR42_RS31820 0.26 -0.3
2,516,209 - RR42_RS31820 0.26 -0.7
2,516,253 - RR42_RS31820 0.32 -0.9
2,516,339 + RR42_RS31820 0.42 -1.0
2,516,784 - -1.6
2,516,902 + +1.1
2,516,902 + -0.6
2,517,212 + RR42_RS31825 0.43 +0.0
2,517,213 - RR42_RS31825 0.43 -0.4
2,517,341 - RR42_RS31825 0.58 -0.7
2,517,554 + RR42_RS31825 0.83 -0.6
2,517,554 + RR42_RS31825 0.83 -0.4
2,517,686 + -1.7

Or see this region's nucleotide sequence