Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS31740

Experiment: R2A with Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS31730 and RR42_RS31735 overlap by 4 nucleotidesRR42_RS31735 and RR42_RS31740 overlap by 4 nucleotidesRR42_RS31740 and RR42_RS31745 are separated by 14 nucleotidesRR42_RS31745 and RR42_RS31750 are separated by 76 nucleotides RR42_RS31730: RR42_RS31730 - formimidoylglutamase, at 2,496,074 to 2,497,015 _RS31730 RR42_RS31735: RR42_RS31735 - ABC transporter ATP-binding protein, at 2,497,012 to 2,497,773 _RS31735 RR42_RS31740: RR42_RS31740 - ABC transporter permease, at 2,497,770 to 2,498,438 _RS31740 RR42_RS31745: RR42_RS31745 - ABC transporter permease, at 2,498,453 to 2,499,202 _RS31745 RR42_RS31750: RR42_RS31750 - IclR family transcriptional regulator, at 2,499,279 to 2,500,046 _RS31750 Position (kb) 2497 2498 2499Strain fitness (log2 ratio) -1 0 1 2at 2497.101 kb on + strand, within RR42_RS31735at 2497.255 kb on - strand, within RR42_RS31735at 2497.255 kb on - strand, within RR42_RS31735at 2497.791 kb on - strandat 2497.849 kb on + strand, within RR42_RS31740at 2497.849 kb on + strand, within RR42_RS31740at 2497.850 kb on - strand, within RR42_RS31740at 2497.850 kb on - strand, within RR42_RS31740at 2497.871 kb on - strand, within RR42_RS31740at 2497.948 kb on - strand, within RR42_RS31740at 2497.948 kb on - strand, within RR42_RS31740at 2498.132 kb on - strand, within RR42_RS31740at 2498.282 kb on - strand, within RR42_RS31740at 2498.282 kb on - strand, within RR42_RS31740at 2498.455 kb on - strandat 2498.455 kb on - strandat 2498.455 kb on - strandat 2498.458 kb on + strandat 2498.458 kb on + strandat 2498.458 kb on + strandat 2498.458 kb on + strandat 2498.497 kb on - strandat 2498.581 kb on - strand, within RR42_RS31745at 2498.637 kb on + strand, within RR42_RS31745at 2498.637 kb on + strand, within RR42_RS31745at 2498.637 kb on + strand, within RR42_RS31745at 2498.638 kb on - strand, within RR42_RS31745at 2498.638 kb on - strand, within RR42_RS31745at 2498.746 kb on - strand, within RR42_RS31745at 2498.746 kb on - strand, within RR42_RS31745at 2498.746 kb on - strand, within RR42_RS31745at 2498.793 kb on - strand, within RR42_RS31745at 2498.793 kb on - strand, within RR42_RS31745at 2498.821 kb on + strand, within RR42_RS31745at 2498.874 kb on + strand, within RR42_RS31745at 2498.875 kb on - strand, within RR42_RS31745at 2498.919 kb on + strand, within RR42_RS31745at 2498.919 kb on + strand, within RR42_RS31745at 2498.919 kb on + strand, within RR42_RS31745at 2498.919 kb on + strand, within RR42_RS31745at 2498.920 kb on - strand, within RR42_RS31745at 2498.920 kb on - strand, within RR42_RS31745at 2499.218 kb on + strandat 2499.219 kb on - strandat 2499.222 kb on + strandat 2499.223 kb on - strandat 2499.241 kb on + strandat 2499.280 kb on + strandat 2499.280 kb on + strandat 2499.281 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml
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2,497,101 + RR42_RS31735 0.12 +0.0
2,497,255 - RR42_RS31735 0.32 -1.0
2,497,255 - RR42_RS31735 0.32 -0.1
2,497,791 - +0.6
2,497,849 + RR42_RS31740 0.12 -0.6
2,497,849 + RR42_RS31740 0.12 +0.4
2,497,850 - RR42_RS31740 0.12 +0.9
2,497,850 - RR42_RS31740 0.12 -1.2
2,497,871 - RR42_RS31740 0.15 +0.9
2,497,948 - RR42_RS31740 0.27 +1.8
2,497,948 - RR42_RS31740 0.27 -0.5
2,498,132 - RR42_RS31740 0.54 -1.1
2,498,282 - RR42_RS31740 0.77 -0.5
2,498,282 - RR42_RS31740 0.77 +0.3
2,498,455 - +0.7
2,498,455 - +0.4
2,498,455 - -1.4
2,498,458 + -0.7
2,498,458 + -0.5
2,498,458 + -1.2
2,498,458 + -0.5
2,498,497 - -0.2
2,498,581 - RR42_RS31745 0.17 -0.5
2,498,637 + RR42_RS31745 0.25 +0.2
2,498,637 + RR42_RS31745 0.25 +1.3
2,498,637 + RR42_RS31745 0.25 -0.2
2,498,638 - RR42_RS31745 0.25 +0.1
2,498,638 - RR42_RS31745 0.25 +1.7
2,498,746 - RR42_RS31745 0.39 -0.1
2,498,746 - RR42_RS31745 0.39 +0.4
2,498,746 - RR42_RS31745 0.39 +1.1
2,498,793 - RR42_RS31745 0.45 +2.6
2,498,793 - RR42_RS31745 0.45 -0.7
2,498,821 + RR42_RS31745 0.49 -1.0
2,498,874 + RR42_RS31745 0.56 -0.8
2,498,875 - RR42_RS31745 0.56 -1.6
2,498,919 + RR42_RS31745 0.62 -0.7
2,498,919 + RR42_RS31745 0.62 +0.6
2,498,919 + RR42_RS31745 0.62 -1.3
2,498,919 + RR42_RS31745 0.62 -0.0
2,498,920 - RR42_RS31745 0.62 +0.9
2,498,920 - RR42_RS31745 0.62 -0.1
2,499,218 + -0.5
2,499,219 - +0.2
2,499,222 + -0.5
2,499,223 - -1.4
2,499,241 + +0.5
2,499,280 + +0.3
2,499,280 + +0.3
2,499,281 - +0.3

Or see this region's nucleotide sequence