Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31560 and RR42_RS31565 are separated by 194 nucleotides RR42_RS31565 and RR42_RS31570 are separated by 200 nucleotides RR42_RS31570 and RR42_RS31575 are separated by 151 nucleotides RR42_RS31575 and RR42_RS31580 are separated by 73 nucleotides
RR42_RS31560: RR42_RS31560 - TetR family transcriptional regulator, at 2,460,766 to 2,461,326
_RS31560
RR42_RS31565: RR42_RS31565 - 50S ribosomal protein L21, at 2,461,521 to 2,462,000
_RS31565
RR42_RS31570: RR42_RS31570 - Asp/Glu/hydantoin racemase, at 2,462,201 to 2,462,821
_RS31570
RR42_RS31575: RR42_RS31575 - GntR family transcriptional regulator, at 2,462,973 to 2,463,656
_RS31575
RR42_RS31580: RR42_RS31580 - 3-hydroxybutyryl-CoA dehydratase, at 2,463,730 to 2,464,494
_RS31580
Position (kb)
2462
2463 Strain fitness (log2 ratio)
-1
0
1
2 at 2461.388 kb on - strand at 2461.502 kb on + strand at 2461.502 kb on + strand at 2461.502 kb on + strand at 2461.502 kb on + strand at 2461.523 kb on - strand at 2461.523 kb on - strand at 2461.523 kb on - strand at 2461.679 kb on - strand, within RR42_RS31565 at 2461.679 kb on - strand, within RR42_RS31565 at 2461.688 kb on - strand, within RR42_RS31565 at 2461.718 kb on - strand, within RR42_RS31565 at 2461.718 kb on - strand, within RR42_RS31565 at 2461.729 kb on - strand, within RR42_RS31565 at 2461.876 kb on + strand, within RR42_RS31565 at 2461.876 kb on + strand, within RR42_RS31565 at 2461.877 kb on - strand, within RR42_RS31565 at 2461.932 kb on + strand, within RR42_RS31565 at 2462.301 kb on + strand, within RR42_RS31570 at 2462.301 kb on + strand, within RR42_RS31570 at 2462.301 kb on + strand, within RR42_RS31570 at 2462.302 kb on - strand, within RR42_RS31570 at 2462.602 kb on + strand, within RR42_RS31570 at 2462.622 kb on + strand, within RR42_RS31570 at 2462.658 kb on + strand, within RR42_RS31570 at 2462.658 kb on + strand, within RR42_RS31570 at 2462.680 kb on + strand, within RR42_RS31570 at 2462.680 kb on + strand, within RR42_RS31570 at 2462.680 kb on + strand, within RR42_RS31570 at 2462.680 kb on + strand, within RR42_RS31570 at 2462.681 kb on - strand, within RR42_RS31570 at 2462.681 kb on - strand, within RR42_RS31570 at 2462.682 kb on + strand, within RR42_RS31570 at 2462.683 kb on - strand, within RR42_RS31570 at 2462.683 kb on - strand, within RR42_RS31570 at 2462.799 kb on + strand at 2462.806 kb on - strand at 2462.931 kb on + strand at 2463.007 kb on + strand at 2463.008 kb on - strand at 2463.188 kb on - strand, within RR42_RS31575 at 2463.232 kb on + strand, within RR42_RS31575 at 2463.233 kb on - strand, within RR42_RS31575
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,461,388 - -0.2 2,461,502 + +1.1 2,461,502 + +0.0 2,461,502 + +1.4 2,461,502 + -0.4 2,461,523 - +0.6 2,461,523 - +1.0 2,461,523 - -0.2 2,461,679 - RR42_RS31565 0.33 -0.2 2,461,679 - RR42_RS31565 0.33 -0.2 2,461,688 - RR42_RS31565 0.35 -1.6 2,461,718 - RR42_RS31565 0.41 +0.1 2,461,718 - RR42_RS31565 0.41 -1.0 2,461,729 - RR42_RS31565 0.43 +0.3 2,461,876 + RR42_RS31565 0.74 -1.1 2,461,876 + RR42_RS31565 0.74 +1.9 2,461,877 - RR42_RS31565 0.74 +0.0 2,461,932 + RR42_RS31565 0.86 +0.2 2,462,301 + RR42_RS31570 0.16 -0.3 2,462,301 + RR42_RS31570 0.16 -0.3 2,462,301 + RR42_RS31570 0.16 +1.0 2,462,302 - RR42_RS31570 0.16 +0.4 2,462,602 + RR42_RS31570 0.65 -1.4 2,462,622 + RR42_RS31570 0.68 -0.0 2,462,658 + RR42_RS31570 0.74 +0.0 2,462,658 + RR42_RS31570 0.74 +0.7 2,462,680 + RR42_RS31570 0.77 +1.4 2,462,680 + RR42_RS31570 0.77 +0.6 2,462,680 + RR42_RS31570 0.77 -0.3 2,462,680 + RR42_RS31570 0.77 +0.2 2,462,681 - RR42_RS31570 0.77 +0.5 2,462,681 - RR42_RS31570 0.77 -0.0 2,462,682 + RR42_RS31570 0.77 +1.6 2,462,683 - RR42_RS31570 0.78 -0.2 2,462,683 - RR42_RS31570 0.78 +1.0 2,462,799 + +0.6 2,462,806 - +1.7 2,462,931 + -0.6 2,463,007 + -0.0 2,463,008 - +0.7 2,463,188 - RR42_RS31575 0.31 +1.0 2,463,232 + RR42_RS31575 0.38 -0.5 2,463,233 - RR42_RS31575 0.38 +0.6
Or see this region's nucleotide sequence