Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31480 and RR42_RS31485 are separated by 133 nucleotides RR42_RS31485 and RR42_RS31490 are separated by 401 nucleotides RR42_RS31490 and RR42_RS31495 are separated by 32 nucleotides RR42_RS31495 and RR42_RS31500 are separated by 224 nucleotides RR42_RS31500 and RR42_RS31505 are separated by 112 nucleotides
RR42_RS31480: RR42_RS31480 - polyphosphate kinase, at 2,442,790 to 2,444,817
_RS31480
RR42_RS31485: RR42_RS31485 - membrane protein, at 2,444,951 to 2,445,295
_RS31485
RR42_RS31490: RR42_RS31490 - hypothetical protein, at 2,445,697 to 2,446,218
_RS31490
RR42_RS31495: RR42_RS31495 - Cro/Cl family transcriptional regulator, at 2,446,251 to 2,446,478
_RS31495
RR42_RS31500: RR42_RS31500 - hypothetical protein, at 2,446,703 to 2,446,963
_RS31500
RR42_RS31505: RR42_RS31505 - hypothetical protein, at 2,447,076 to 2,447,405
_RS31505
Position (kb)
2445
2446
2447 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2444.705 kb on - strand at 2444.753 kb on - strand at 2444.856 kb on + strand at 2444.889 kb on + strand at 2444.889 kb on + strand at 2444.890 kb on - strand at 2444.913 kb on + strand at 2444.914 kb on - strand at 2444.952 kb on + strand at 2444.953 kb on - strand at 2444.980 kb on - strand at 2444.980 kb on - strand at 2445.060 kb on + strand, within RR42_RS31485 at 2445.189 kb on + strand, within RR42_RS31485 at 2445.189 kb on + strand, within RR42_RS31485 at 2445.189 kb on + strand, within RR42_RS31485 at 2445.189 kb on + strand, within RR42_RS31485 at 2445.190 kb on - strand, within RR42_RS31485 at 2445.190 kb on - strand, within RR42_RS31485 at 2445.190 kb on - strand, within RR42_RS31485 at 2445.328 kb on + strand at 2445.329 kb on - strand at 2445.329 kb on - strand at 2445.329 kb on - strand at 2445.352 kb on - strand at 2445.393 kb on + strand at 2445.393 kb on + strand at 2445.394 kb on - strand at 2445.394 kb on - strand at 2445.414 kb on + strand at 2445.415 kb on - strand at 2445.434 kb on + strand at 2445.434 kb on + strand at 2445.435 kb on - strand at 2445.435 kb on - strand at 2445.435 kb on - strand at 2445.440 kb on + strand at 2445.441 kb on - strand at 2445.441 kb on - strand at 2445.696 kb on - strand at 2446.105 kb on + strand, within RR42_RS31490 at 2446.106 kb on - strand, within RR42_RS31490 at 2446.106 kb on - strand, within RR42_RS31490 at 2446.250 kb on + strand at 2446.250 kb on + strand at 2446.250 kb on + strand at 2446.250 kb on + strand at 2446.251 kb on - strand at 2446.251 kb on - strand at 2446.251 kb on - strand at 2446.257 kb on - strand at 2446.388 kb on + strand, within RR42_RS31495 at 2446.388 kb on + strand, within RR42_RS31495 at 2446.455 kb on - strand, within RR42_RS31495 at 2446.649 kb on + strand at 2446.649 kb on + strand at 2446.668 kb on + strand at 2446.668 kb on + strand at 2446.668 kb on + strand at 2446.669 kb on - strand at 2446.702 kb on + strand at 2446.703 kb on - strand at 2446.703 kb on - strand at 2446.703 kb on - strand at 2446.703 kb on - strand at 2446.703 kb on - strand at 2446.718 kb on + strand at 2446.718 kb on + strand at 2446.719 kb on - strand at 2446.719 kb on - strand at 2446.719 kb on - strand at 2446.983 kb on + strand at 2447.026 kb on + strand at 2447.027 kb on - strand at 2447.106 kb on + strand at 2447.106 kb on + strand at 2447.107 kb on - strand at 2447.107 kb on - strand at 2447.107 kb on - strand at 2447.144 kb on - strand, within RR42_RS31505 at 2447.199 kb on + strand, within RR42_RS31505
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,444,705 - -0.7 2,444,753 - -0.0 2,444,856 + +0.5 2,444,889 + +0.9 2,444,889 + -1.8 2,444,890 - +1.2 2,444,913 + +0.4 2,444,914 - +0.8 2,444,952 + -0.1 2,444,953 - -0.2 2,444,980 - -2.1 2,444,980 - -1.1 2,445,060 + RR42_RS31485 0.32 -0.7 2,445,189 + RR42_RS31485 0.69 -0.5 2,445,189 + RR42_RS31485 0.69 -0.4 2,445,189 + RR42_RS31485 0.69 +0.6 2,445,189 + RR42_RS31485 0.69 -2.5 2,445,190 - RR42_RS31485 0.69 -0.2 2,445,190 - RR42_RS31485 0.69 +0.6 2,445,190 - RR42_RS31485 0.69 +1.3 2,445,328 + -0.1 2,445,329 - -0.1 2,445,329 - +0.2 2,445,329 - +1.7 2,445,352 - -0.2 2,445,393 + -0.6 2,445,393 + +0.0 2,445,394 - -0.1 2,445,394 - -0.1 2,445,414 + -2.1 2,445,415 - +0.5 2,445,434 + +0.1 2,445,434 + -1.8 2,445,435 - -0.0 2,445,435 - +0.2 2,445,435 - -0.8 2,445,440 + +0.0 2,445,441 - +1.1 2,445,441 - -0.8 2,445,696 - +0.6 2,446,105 + RR42_RS31490 0.78 +1.0 2,446,106 - RR42_RS31490 0.78 -1.3 2,446,106 - RR42_RS31490 0.78 -0.7 2,446,250 + -0.4 2,446,250 + -0.1 2,446,250 + -0.7 2,446,250 + -1.0 2,446,251 - +1.4 2,446,251 - -0.6 2,446,251 - -0.7 2,446,257 - -0.4 2,446,388 + RR42_RS31495 0.60 +2.0 2,446,388 + RR42_RS31495 0.60 +1.6 2,446,455 - RR42_RS31495 0.89 -0.4 2,446,649 + +2.1 2,446,649 + +0.4 2,446,668 + -0.7 2,446,668 + -0.1 2,446,668 + -0.7 2,446,669 - -1.1 2,446,702 + -0.2 2,446,703 - -0.1 2,446,703 - -0.3 2,446,703 - -0.6 2,446,703 - -0.9 2,446,703 - +0.3 2,446,718 + -0.8 2,446,718 + -0.1 2,446,719 - -0.7 2,446,719 - +0.7 2,446,719 - +0.2 2,446,983 + -1.7 2,447,026 + +0.9 2,447,027 - +0.5 2,447,106 + -0.2 2,447,106 + -1.4 2,447,107 - +0.2 2,447,107 - +1.0 2,447,107 - -1.3 2,447,144 - RR42_RS31505 0.21 -1.4 2,447,199 + RR42_RS31505 0.37 +0.8
Or see this region's nucleotide sequence