Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31365 and RR42_RS31370 are separated by 23 nucleotides RR42_RS31370 and RR42_RS31375 are separated by 466 nucleotides
RR42_RS31365: RR42_RS31365 - formyl-CoA transferase, at 2,412,316 to 2,413,545
_RS31365
RR42_RS31370: RR42_RS31370 - acetolactate synthase, at 2,413,569 to 2,415,323
_RS31370
RR42_RS31375: RR42_RS31375 - succinyl-CoA synthetase subunit beta, at 2,415,790 to 2,417,022
_RS31375
Position (kb)
2413
2414
2415
2416 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8
9 at 2412.768 kb on - strand, within RR42_RS31365 at 2412.768 kb on - strand, within RR42_RS31365 at 2412.872 kb on + strand, within RR42_RS31365 at 2412.872 kb on + strand, within RR42_RS31365 at 2413.123 kb on + strand, within RR42_RS31365 at 2413.124 kb on - strand, within RR42_RS31365 at 2413.184 kb on + strand, within RR42_RS31365 at 2413.184 kb on + strand, within RR42_RS31365 at 2413.185 kb on - strand, within RR42_RS31365 at 2413.185 kb on - strand, within RR42_RS31365 at 2413.185 kb on - strand, within RR42_RS31365 at 2413.185 kb on - strand, within RR42_RS31365 at 2413.185 kb on - strand, within RR42_RS31365 at 2413.327 kb on - strand, within RR42_RS31365 at 2413.327 kb on - strand, within RR42_RS31365 at 2413.371 kb on - strand, within RR42_RS31365 at 2413.371 kb on - strand, within RR42_RS31365 at 2413.571 kb on - strand at 2413.576 kb on + strand at 2413.576 kb on + strand at 2413.576 kb on + strand at 2413.576 kb on + strand at 2413.576 kb on + strand at 2413.576 kb on + strand at 2413.577 kb on - strand at 2413.577 kb on - strand at 2413.577 kb on - strand at 2413.577 kb on - strand at 2413.628 kb on + strand at 2413.628 kb on + strand at 2413.629 kb on - strand at 2413.629 kb on - strand at 2413.629 kb on - strand at 2413.801 kb on + strand, within RR42_RS31370 at 2413.801 kb on + strand, within RR42_RS31370 at 2413.931 kb on - strand, within RR42_RS31370 at 2413.933 kb on + strand, within RR42_RS31370 at 2413.934 kb on - strand, within RR42_RS31370 at 2413.934 kb on - strand, within RR42_RS31370 at 2414.096 kb on - strand, within RR42_RS31370 at 2414.132 kb on - strand, within RR42_RS31370 at 2414.132 kb on - strand, within RR42_RS31370 at 2414.132 kb on - strand, within RR42_RS31370 at 2414.617 kb on + strand, within RR42_RS31370 at 2414.739 kb on + strand, within RR42_RS31370 at 2414.825 kb on + strand, within RR42_RS31370 at 2414.826 kb on - strand, within RR42_RS31370 at 2415.073 kb on + strand, within RR42_RS31370 at 2415.151 kb on + strand at 2415.155 kb on + strand at 2415.192 kb on - strand at 2415.570 kb on - strand at 2415.591 kb on + strand at 2415.771 kb on + strand at 2415.805 kb on + strand at 2415.806 kb on - strand at 2415.995 kb on + strand, within RR42_RS31375 at 2416.103 kb on + strand, within RR42_RS31375 at 2416.103 kb on + strand, within RR42_RS31375 at 2416.103 kb on + strand, within RR42_RS31375 at 2416.104 kb on - strand, within RR42_RS31375 at 2416.104 kb on - strand, within RR42_RS31375 at 2416.105 kb on + strand, within RR42_RS31375 at 2416.106 kb on - strand, within RR42_RS31375 at 2416.106 kb on - strand, within RR42_RS31375 at 2416.106 kb on - strand, within RR42_RS31375 at 2416.106 kb on - strand, within RR42_RS31375 at 2416.139 kb on + strand, within RR42_RS31375 at 2416.139 kb on + strand, within RR42_RS31375 at 2416.142 kb on - strand, within RR42_RS31375
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,412,768 - RR42_RS31365 0.37 -0.1 2,412,768 - RR42_RS31365 0.37 +0.4 2,412,872 + RR42_RS31365 0.45 -1.1 2,412,872 + RR42_RS31365 0.45 -1.2 2,413,123 + RR42_RS31365 0.66 -0.8 2,413,124 - RR42_RS31365 0.66 +0.9 2,413,184 + RR42_RS31365 0.71 +0.6 2,413,184 + RR42_RS31365 0.71 -0.2 2,413,185 - RR42_RS31365 0.71 +1.5 2,413,185 - RR42_RS31365 0.71 -0.4 2,413,185 - RR42_RS31365 0.71 -0.2 2,413,185 - RR42_RS31365 0.71 -0.8 2,413,185 - RR42_RS31365 0.71 +3.2 2,413,327 - RR42_RS31365 0.82 -1.3 2,413,327 - RR42_RS31365 0.82 -0.4 2,413,371 - RR42_RS31365 0.86 +0.0 2,413,371 - RR42_RS31365 0.86 +1.0 2,413,571 - -0.8 2,413,576 + +0.1 2,413,576 + +0.5 2,413,576 + +0.3 2,413,576 + -1.0 2,413,576 + -2.1 2,413,576 + -1.1 2,413,577 - +2.1 2,413,577 - -0.0 2,413,577 - -0.4 2,413,577 - -0.2 2,413,628 + +2.0 2,413,628 + -0.9 2,413,629 - -1.0 2,413,629 - -0.1 2,413,629 - -1.2 2,413,801 + RR42_RS31370 0.13 -0.8 2,413,801 + RR42_RS31370 0.13 +0.5 2,413,931 - RR42_RS31370 0.21 +0.7 2,413,933 + RR42_RS31370 0.21 -0.7 2,413,934 - RR42_RS31370 0.21 -0.5 2,413,934 - RR42_RS31370 0.21 +1.0 2,414,096 - RR42_RS31370 0.30 -0.2 2,414,132 - RR42_RS31370 0.32 -0.2 2,414,132 - RR42_RS31370 0.32 -1.8 2,414,132 - RR42_RS31370 0.32 +1.4 2,414,617 + RR42_RS31370 0.60 +0.9 2,414,739 + RR42_RS31370 0.67 -1.3 2,414,825 + RR42_RS31370 0.72 -0.6 2,414,826 - RR42_RS31370 0.72 +0.6 2,415,073 + RR42_RS31370 0.86 -0.7 2,415,151 + +0.2 2,415,155 + +0.2 2,415,192 - +0.8 2,415,570 - +0.8 2,415,591 + -1.3 2,415,771 + +0.5 2,415,805 + -0.5 2,415,806 - +0.9 2,415,995 + RR42_RS31375 0.17 +9.6 2,416,103 + RR42_RS31375 0.25 -2.5 2,416,103 + RR42_RS31375 0.25 -1.0 2,416,103 + RR42_RS31375 0.25 -0.2 2,416,104 - RR42_RS31375 0.25 -1.0 2,416,104 - RR42_RS31375 0.25 -0.4 2,416,105 + RR42_RS31375 0.26 +1.3 2,416,106 - RR42_RS31375 0.26 +0.2 2,416,106 - RR42_RS31375 0.26 -0.0 2,416,106 - RR42_RS31375 0.26 +0.4 2,416,106 - RR42_RS31375 0.26 +1.9 2,416,139 + RR42_RS31375 0.28 +0.2 2,416,139 + RR42_RS31375 0.28 -0.3 2,416,142 - RR42_RS31375 0.29 -0.3
Or see this region's nucleotide sequence