Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31265 and RR42_RS31270 are separated by 622 nucleotides RR42_RS31270 and RR42_RS31275 are separated by 105 nucleotides
RR42_RS31265: RR42_RS31265 - hypothetical protein, at 2,388,742 to 2,389,470
_RS31265
RR42_RS31270: RR42_RS31270 - fumarylacetoacetase, at 2,390,093 to 2,391,355
_RS31270
RR42_RS31275: RR42_RS31275 - Homogentisate 1,2-dioxygenase (EC 1.13.11.5) (from data) , at 2,391,461 to 2,392,810
_RS31275
Position (kb)
2390
2391
2392 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2389.127 kb on + strand, within RR42_RS31265 at 2389.147 kb on - strand, within RR42_RS31265 at 2389.286 kb on + strand, within RR42_RS31265 at 2389.286 kb on + strand, within RR42_RS31265 at 2389.286 kb on + strand, within RR42_RS31265 at 2389.287 kb on - strand, within RR42_RS31265 at 2389.427 kb on - strand at 2389.427 kb on - strand at 2389.427 kb on - strand at 2389.487 kb on + strand at 2389.488 kb on - strand at 2389.488 kb on - strand at 2389.750 kb on - strand at 2389.762 kb on - strand at 2389.830 kb on + strand at 2389.830 kb on + strand at 2389.831 kb on - strand at 2389.831 kb on - strand at 2389.831 kb on - strand at 2389.831 kb on - strand at 2389.920 kb on + strand at 2389.921 kb on - strand at 2389.972 kb on - strand at 2390.037 kb on - strand at 2390.094 kb on + strand at 2390.376 kb on + strand, within RR42_RS31270 at 2390.376 kb on + strand, within RR42_RS31270 at 2390.376 kb on + strand, within RR42_RS31270 at 2390.376 kb on + strand, within RR42_RS31270 at 2390.377 kb on - strand, within RR42_RS31270 at 2390.377 kb on - strand, within RR42_RS31270 at 2390.461 kb on - strand, within RR42_RS31270 at 2390.461 kb on - strand, within RR42_RS31270 at 2390.461 kb on - strand, within RR42_RS31270 at 2390.461 kb on - strand, within RR42_RS31270 at 2390.613 kb on + strand, within RR42_RS31270 at 2390.613 kb on + strand, within RR42_RS31270 at 2390.614 kb on - strand, within RR42_RS31270 at 2390.752 kb on - strand, within RR42_RS31270 at 2390.752 kb on - strand, within RR42_RS31270 at 2391.242 kb on + strand at 2391.280 kb on - strand at 2391.280 kb on - strand at 2391.510 kb on + strand at 2391.510 kb on + strand at 2391.511 kb on - strand at 2391.511 kb on - strand at 2391.513 kb on + strand at 2391.514 kb on - strand at 2391.547 kb on - strand at 2391.547 kb on - strand at 2391.622 kb on + strand, within RR42_RS31275 at 2391.729 kb on + strand, within RR42_RS31275 at 2391.730 kb on - strand, within RR42_RS31275 at 2391.928 kb on + strand, within RR42_RS31275 at 2391.929 kb on - strand, within RR42_RS31275 at 2391.954 kb on + strand, within RR42_RS31275 at 2391.963 kb on + strand, within RR42_RS31275 at 2391.964 kb on - strand, within RR42_RS31275 at 2392.071 kb on + strand, within RR42_RS31275 at 2392.071 kb on + strand, within RR42_RS31275 at 2392.072 kb on - strand, within RR42_RS31275 at 2392.072 kb on - strand, within RR42_RS31275 at 2392.072 kb on - strand, within RR42_RS31275 at 2392.161 kb on + strand, within RR42_RS31275 at 2392.161 kb on + strand, within RR42_RS31275 at 2392.162 kb on - strand, within RR42_RS31275 at 2392.174 kb on + strand, within RR42_RS31275 at 2392.175 kb on - strand, within RR42_RS31275 at 2392.175 kb on - strand, within RR42_RS31275 at 2392.175 kb on - strand, within RR42_RS31275 at 2392.176 kb on + strand, within RR42_RS31275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,389,127 + RR42_RS31265 0.53 -1.0 2,389,147 - RR42_RS31265 0.56 +1.7 2,389,286 + RR42_RS31265 0.75 -0.0 2,389,286 + RR42_RS31265 0.75 -0.4 2,389,286 + RR42_RS31265 0.75 -1.4 2,389,287 - RR42_RS31265 0.75 +1.4 2,389,427 - -0.4 2,389,427 - -1.6 2,389,427 - +0.5 2,389,487 + -0.2 2,389,488 - +3.3 2,389,488 - -0.0 2,389,750 - -0.5 2,389,762 - -0.6 2,389,830 + +1.3 2,389,830 + +0.1 2,389,831 - +0.7 2,389,831 - -0.6 2,389,831 - +0.1 2,389,831 - -0.4 2,389,920 + -0.1 2,389,921 - +1.6 2,389,972 - +0.8 2,390,037 - -0.0 2,390,094 + -0.3 2,390,376 + RR42_RS31270 0.22 +1.6 2,390,376 + RR42_RS31270 0.22 +1.0 2,390,376 + RR42_RS31270 0.22 +1.1 2,390,376 + RR42_RS31270 0.22 +0.5 2,390,377 - RR42_RS31270 0.22 -1.1 2,390,377 - RR42_RS31270 0.22 +1.6 2,390,461 - RR42_RS31270 0.29 +0.6 2,390,461 - RR42_RS31270 0.29 -1.4 2,390,461 - RR42_RS31270 0.29 +0.1 2,390,461 - RR42_RS31270 0.29 -0.9 2,390,613 + RR42_RS31270 0.41 +0.4 2,390,613 + RR42_RS31270 0.41 -1.1 2,390,614 - RR42_RS31270 0.41 -1.0 2,390,752 - RR42_RS31270 0.52 -2.2 2,390,752 - RR42_RS31270 0.52 +0.7 2,391,242 + +1.6 2,391,280 - -0.3 2,391,280 - +2.2 2,391,510 + +1.6 2,391,510 + -0.2 2,391,511 - -0.2 2,391,511 - -0.1 2,391,513 + +1.3 2,391,514 - -1.4 2,391,547 - -0.5 2,391,547 - -0.6 2,391,622 + RR42_RS31275 0.12 -0.4 2,391,729 + RR42_RS31275 0.20 -0.6 2,391,730 - RR42_RS31275 0.20 -1.3 2,391,928 + RR42_RS31275 0.35 -1.0 2,391,929 - RR42_RS31275 0.35 +0.6 2,391,954 + RR42_RS31275 0.37 -0.2 2,391,963 + RR42_RS31275 0.37 -0.7 2,391,964 - RR42_RS31275 0.37 -0.0 2,392,071 + RR42_RS31275 0.45 -0.2 2,392,071 + RR42_RS31275 0.45 +0.4 2,392,072 - RR42_RS31275 0.45 -0.1 2,392,072 - RR42_RS31275 0.45 +1.2 2,392,072 - RR42_RS31275 0.45 +0.2 2,392,161 + RR42_RS31275 0.52 +0.2 2,392,161 + RR42_RS31275 0.52 -1.0 2,392,162 - RR42_RS31275 0.52 +1.2 2,392,174 + RR42_RS31275 0.53 +0.0 2,392,175 - RR42_RS31275 0.53 +1.0 2,392,175 - RR42_RS31275 0.53 +0.6 2,392,175 - RR42_RS31275 0.53 -1.2 2,392,176 + RR42_RS31275 0.53 -0.0
Or see this region's nucleotide sequence