Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31215 and RR42_RS31220 are separated by 70 nucleotides RR42_RS31220 and RR42_RS31225 overlap by 4 nucleotides RR42_RS31225 and RR42_RS31230 are separated by 91 nucleotides
RR42_RS31215: RR42_RS31215 - membrane protein, at 2,379,855 to 2,382,176
_RS31215
RR42_RS31220: RR42_RS31220 - lipoprotein carrier protein LolA, at 2,382,247 to 2,382,873
_RS31220
RR42_RS31225: RR42_RS31225 - 4-hydroxybenzoyl-CoA thioesterase, at 2,382,870 to 2,383,319
_RS31225
RR42_RS31230: RR42_RS31230 - acyltransferase, at 2,383,411 to 2,384,367
_RS31230
Position (kb)
2382
2383
2384 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2382.940 kb on + strand, within RR42_RS31225 at 2382.941 kb on - strand, within RR42_RS31225 at 2382.941 kb on - strand, within RR42_RS31225 at 2382.941 kb on - strand, within RR42_RS31225 at 2382.941 kb on - strand, within RR42_RS31225 at 2382.986 kb on - strand, within RR42_RS31225 at 2382.986 kb on - strand, within RR42_RS31225 at 2382.986 kb on - strand, within RR42_RS31225 at 2383.030 kb on + strand, within RR42_RS31225 at 2383.093 kb on + strand, within RR42_RS31225 at 2383.094 kb on - strand, within RR42_RS31225 at 2383.098 kb on - strand, within RR42_RS31225 at 2383.098 kb on - strand, within RR42_RS31225 at 2383.111 kb on + strand, within RR42_RS31225 at 2383.111 kb on + strand, within RR42_RS31225 at 2383.111 kb on + strand, within RR42_RS31225 at 2383.112 kb on - strand, within RR42_RS31225 at 2383.112 kb on - strand, within RR42_RS31225 at 2383.112 kb on - strand, within RR42_RS31225 at 2383.112 kb on - strand, within RR42_RS31225 at 2383.112 kb on - strand, within RR42_RS31225 at 2383.144 kb on + strand, within RR42_RS31225 at 2383.174 kb on + strand, within RR42_RS31225 at 2383.174 kb on + strand, within RR42_RS31225 at 2383.225 kb on + strand, within RR42_RS31225 at 2383.225 kb on + strand, within RR42_RS31225 at 2383.226 kb on - strand, within RR42_RS31225 at 2383.319 kb on + strand at 2383.320 kb on - strand at 2383.320 kb on - strand at 2383.421 kb on + strand at 2383.631 kb on + strand, within RR42_RS31230 at 2384.015 kb on + strand, within RR42_RS31230 at 2384.021 kb on + strand, within RR42_RS31230 at 2384.050 kb on + strand, within RR42_RS31230 at 2384.050 kb on + strand, within RR42_RS31230 at 2384.051 kb on - strand, within RR42_RS31230 at 2384.156 kb on + strand, within RR42_RS31230 at 2384.198 kb on + strand, within RR42_RS31230 at 2384.198 kb on + strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.201 kb on + strand, within RR42_RS31230 at 2384.201 kb on + strand, within RR42_RS31230 at 2384.201 kb on + strand, within RR42_RS31230 at 2384.202 kb on - strand, within RR42_RS31230 at 2384.202 kb on - strand, within RR42_RS31230 at 2384.202 kb on - strand, within RR42_RS31230 at 2384.255 kb on + strand, within RR42_RS31230 at 2384.264 kb on + strand, within RR42_RS31230 at 2384.264 kb on + strand, within RR42_RS31230 at 2384.264 kb on + strand, within RR42_RS31230 at 2384.284 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,382,940 + RR42_RS31225 0.16 -0.4 2,382,941 - RR42_RS31225 0.16 -0.9 2,382,941 - RR42_RS31225 0.16 -1.3 2,382,941 - RR42_RS31225 0.16 -1.4 2,382,941 - RR42_RS31225 0.16 +0.5 2,382,986 - RR42_RS31225 0.26 -0.2 2,382,986 - RR42_RS31225 0.26 -0.6 2,382,986 - RR42_RS31225 0.26 +0.9 2,383,030 + RR42_RS31225 0.36 -0.0 2,383,093 + RR42_RS31225 0.50 -1.8 2,383,094 - RR42_RS31225 0.50 -0.2 2,383,098 - RR42_RS31225 0.51 +0.1 2,383,098 - RR42_RS31225 0.51 +0.6 2,383,111 + RR42_RS31225 0.54 +0.5 2,383,111 + RR42_RS31225 0.54 +0.5 2,383,111 + RR42_RS31225 0.54 -1.4 2,383,112 - RR42_RS31225 0.54 +1.2 2,383,112 - RR42_RS31225 0.54 -1.3 2,383,112 - RR42_RS31225 0.54 -0.5 2,383,112 - RR42_RS31225 0.54 +2.6 2,383,112 - RR42_RS31225 0.54 -0.5 2,383,144 + RR42_RS31225 0.61 +1.0 2,383,174 + RR42_RS31225 0.68 -0.9 2,383,174 + RR42_RS31225 0.68 -0.5 2,383,225 + RR42_RS31225 0.79 +1.8 2,383,225 + RR42_RS31225 0.79 +0.5 2,383,226 - RR42_RS31225 0.79 -1.2 2,383,319 + -0.6 2,383,320 - +0.6 2,383,320 - +0.5 2,383,421 + +0.5 2,383,631 + RR42_RS31230 0.23 +0.2 2,384,015 + RR42_RS31230 0.63 -0.2 2,384,021 + RR42_RS31230 0.64 -0.4 2,384,050 + RR42_RS31230 0.67 +0.1 2,384,050 + RR42_RS31230 0.67 +1.8 2,384,051 - RR42_RS31230 0.67 -0.4 2,384,156 + RR42_RS31230 0.78 -0.0 2,384,198 + RR42_RS31230 0.82 -0.4 2,384,198 + RR42_RS31230 0.82 -1.0 2,384,199 - RR42_RS31230 0.82 +2.2 2,384,199 - RR42_RS31230 0.82 -1.1 2,384,199 - RR42_RS31230 0.82 -0.2 2,384,199 - RR42_RS31230 0.82 -0.5 2,384,199 - RR42_RS31230 0.82 -1.5 2,384,201 + RR42_RS31230 0.83 +1.4 2,384,201 + RR42_RS31230 0.83 -0.1 2,384,201 + RR42_RS31230 0.83 -1.0 2,384,202 - RR42_RS31230 0.83 -1.0 2,384,202 - RR42_RS31230 0.83 -0.2 2,384,202 - RR42_RS31230 0.83 +0.0 2,384,255 + RR42_RS31230 0.88 -0.9 2,384,264 + RR42_RS31230 0.89 -0.8 2,384,264 + RR42_RS31230 0.89 +1.6 2,384,264 + RR42_RS31230 0.89 -0.8 2,384,284 + -1.6
Or see this region's nucleotide sequence