Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31055 and RR42_RS31060 are separated by 90 nucleotides RR42_RS31060 and RR42_RS31065 are separated by 250 nucleotides RR42_RS31065 and RR42_RS31070 are separated by 58 nucleotides
RR42_RS31055: RR42_RS31055 - 3-hydroxybutyryl-CoA dehydrogenase, at 2,347,160 to 2,348,086
_RS31055
RR42_RS31060: RR42_RS31060 - diguanylate cyclase, at 2,348,177 to 2,349,748
_RS31060
RR42_RS31065: RR42_RS31065 - GntR family transcriptional regulator, at 2,349,999 to 2,350,673
_RS31065
RR42_RS31070: RR42_RS31070 - dihydroxy-acid dehydratase, at 2,350,732 to 2,352,462
_RS31070
Position (kb)
2348
2349
2350 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2347.640 kb on + strand, within RR42_RS31055 at 2347.931 kb on - strand, within RR42_RS31055 at 2348.148 kb on + strand at 2348.253 kb on + strand at 2348.253 kb on + strand at 2348.253 kb on + strand at 2348.254 kb on - strand at 2348.254 kb on - strand at 2348.500 kb on + strand, within RR42_RS31060 at 2348.500 kb on + strand, within RR42_RS31060 at 2348.501 kb on - strand, within RR42_RS31060 at 2348.501 kb on - strand, within RR42_RS31060 at 2348.613 kb on + strand, within RR42_RS31060 at 2348.614 kb on - strand, within RR42_RS31060 at 2348.614 kb on - strand, within RR42_RS31060 at 2348.760 kb on + strand, within RR42_RS31060 at 2348.760 kb on + strand, within RR42_RS31060 at 2348.760 kb on + strand, within RR42_RS31060 at 2348.760 kb on + strand, within RR42_RS31060 at 2348.761 kb on - strand, within RR42_RS31060 at 2348.761 kb on - strand, within RR42_RS31060 at 2348.872 kb on + strand, within RR42_RS31060 at 2348.872 kb on + strand, within RR42_RS31060 at 2348.872 kb on + strand, within RR42_RS31060 at 2348.873 kb on - strand, within RR42_RS31060 at 2348.873 kb on - strand, within RR42_RS31060 at 2349.006 kb on + strand at 2349.041 kb on + strand, within RR42_RS31060 at 2349.041 kb on + strand, within RR42_RS31060 at 2349.071 kb on + strand, within RR42_RS31060 at 2349.149 kb on - strand, within RR42_RS31060 at 2349.168 kb on + strand, within RR42_RS31060 at 2349.168 kb on + strand, within RR42_RS31060 at 2349.169 kb on - strand, within RR42_RS31060 at 2349.169 kb on - strand, within RR42_RS31060 at 2349.293 kb on - strand, within RR42_RS31060 at 2349.298 kb on + strand, within RR42_RS31060 at 2349.323 kb on - strand, within RR42_RS31060 at 2349.323 kb on - strand, within RR42_RS31060 at 2349.425 kb on + strand, within RR42_RS31060 at 2349.520 kb on + strand, within RR42_RS31060 at 2349.520 kb on + strand, within RR42_RS31060 at 2349.521 kb on - strand, within RR42_RS31060 at 2349.537 kb on - strand, within RR42_RS31060 at 2349.537 kb on - strand, within RR42_RS31060 at 2349.537 kb on - strand, within RR42_RS31060 at 2349.553 kb on - strand, within RR42_RS31060 at 2349.553 kb on - strand, within RR42_RS31060 at 2349.615 kb on + strand at 2349.637 kb on + strand at 2349.637 kb on + strand at 2349.678 kb on + strand at 2349.740 kb on + strand at 2349.741 kb on - strand at 2349.741 kb on - strand at 2349.741 kb on - strand at 2349.775 kb on + strand at 2349.775 kb on + strand at 2349.988 kb on - strand at 2349.988 kb on - strand at 2349.997 kb on + strand at 2349.997 kb on + strand at 2349.998 kb on - strand at 2350.000 kb on + strand at 2350.000 kb on + strand at 2350.039 kb on + strand at 2350.039 kb on + strand at 2350.039 kb on + strand at 2350.040 kb on - strand at 2350.074 kb on + strand, within RR42_RS31065 at 2350.075 kb on - strand, within RR42_RS31065 at 2350.204 kb on + strand at 2350.421 kb on - strand, within RR42_RS31065 at 2350.661 kb on + strand at 2350.661 kb on + strand at 2350.676 kb on + strand at 2350.676 kb on + strand at 2350.676 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,347,640 + RR42_RS31055 0.52 +0.6 2,347,931 - RR42_RS31055 0.83 -0.4 2,348,148 + -0.5 2,348,253 + +1.0 2,348,253 + -0.4 2,348,253 + +0.2 2,348,254 - -1.1 2,348,254 - -2.1 2,348,500 + RR42_RS31060 0.21 +0.6 2,348,500 + RR42_RS31060 0.21 +0.2 2,348,501 - RR42_RS31060 0.21 +2.0 2,348,501 - RR42_RS31060 0.21 +0.2 2,348,613 + RR42_RS31060 0.28 -0.7 2,348,614 - RR42_RS31060 0.28 +0.8 2,348,614 - RR42_RS31060 0.28 +0.7 2,348,760 + RR42_RS31060 0.37 +2.6 2,348,760 + RR42_RS31060 0.37 -0.5 2,348,760 + RR42_RS31060 0.37 -1.4 2,348,760 + RR42_RS31060 0.37 -1.1 2,348,761 - RR42_RS31060 0.37 +0.7 2,348,761 - RR42_RS31060 0.37 +0.7 2,348,872 + RR42_RS31060 0.44 +0.6 2,348,872 + RR42_RS31060 0.44 +0.3 2,348,872 + RR42_RS31060 0.44 -2.2 2,348,873 - RR42_RS31060 0.44 -1.0 2,348,873 - RR42_RS31060 0.44 -0.2 2,349,006 + +1.6 2,349,041 + RR42_RS31060 0.55 -0.7 2,349,041 + RR42_RS31060 0.55 +0.9 2,349,071 + RR42_RS31060 0.57 +1.0 2,349,149 - RR42_RS31060 0.62 -1.0 2,349,168 + RR42_RS31060 0.63 -0.1 2,349,168 + RR42_RS31060 0.63 -0.3 2,349,169 - RR42_RS31060 0.63 -0.4 2,349,169 - RR42_RS31060 0.63 -0.0 2,349,293 - RR42_RS31060 0.71 +0.6 2,349,298 + RR42_RS31060 0.71 -1.6 2,349,323 - RR42_RS31060 0.73 -0.2 2,349,323 - RR42_RS31060 0.73 +0.6 2,349,425 + RR42_RS31060 0.79 +0.2 2,349,520 + RR42_RS31060 0.85 -0.4 2,349,520 + RR42_RS31060 0.85 -0.2 2,349,521 - RR42_RS31060 0.85 -0.3 2,349,537 - RR42_RS31060 0.87 -1.7 2,349,537 - RR42_RS31060 0.87 -0.6 2,349,537 - RR42_RS31060 0.87 -1.3 2,349,553 - RR42_RS31060 0.88 -0.7 2,349,553 - RR42_RS31060 0.88 -0.2 2,349,615 + +0.5 2,349,637 + +1.9 2,349,637 + -0.6 2,349,678 + +0.4 2,349,740 + +0.9 2,349,741 - -0.3 2,349,741 - -1.0 2,349,741 - +0.3 2,349,775 + +1.1 2,349,775 + +0.2 2,349,988 - +1.6 2,349,988 - +0.0 2,349,997 + -0.9 2,349,997 + -0.3 2,349,998 - -1.2 2,350,000 + +0.3 2,350,000 + -1.8 2,350,039 + +0.9 2,350,039 + -0.3 2,350,039 + +0.4 2,350,040 - +0.2 2,350,074 + RR42_RS31065 0.11 +0.2 2,350,075 - RR42_RS31065 0.11 +1.0 2,350,204 + +2.6 2,350,421 - RR42_RS31065 0.63 -1.4 2,350,661 + +0.4 2,350,661 + -1.1 2,350,676 + -2.1 2,350,676 + +1.8 2,350,676 + +0.4
Or see this region's nucleotide sequence