Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30505 and RR42_RS30510 are separated by 61 nucleotides RR42_RS30510 and RR42_RS30515 are separated by 41 nucleotides RR42_RS30515 and RR42_RS30520 are separated by 66 nucleotides
RR42_RS30505: RR42_RS30505 - salicylate hydroxylase, at 2,218,035 to 2,219,243
_RS30505
RR42_RS30510: RR42_RS30510 - maleylacetoacetate isomerase, at 2,219,305 to 2,219,949
_RS30510
RR42_RS30515: RR42_RS30515 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 2,219,991 to 2,220,689
_RS30515
RR42_RS30520: RR42_RS30520 - gentisate 1,2-dioxygenase, at 2,220,756 to 2,221,802
_RS30520
Position (kb)
2219
2220
2221 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2219.012 kb on - strand, within RR42_RS30505 at 2219.319 kb on - strand at 2219.559 kb on - strand, within RR42_RS30510 at 2219.606 kb on + strand, within RR42_RS30510 at 2219.606 kb on + strand, within RR42_RS30510 at 2219.735 kb on + strand, within RR42_RS30510 at 2219.822 kb on + strand, within RR42_RS30510 at 2219.822 kb on + strand, within RR42_RS30510 at 2219.822 kb on + strand, within RR42_RS30510 at 2219.822 kb on + strand, within RR42_RS30510 at 2219.823 kb on - strand, within RR42_RS30510 at 2219.937 kb on + strand at 2219.938 kb on - strand at 2219.938 kb on - strand at 2219.939 kb on + strand at 2220.089 kb on - strand, within RR42_RS30515 at 2220.119 kb on - strand, within RR42_RS30515 at 2220.401 kb on + strand, within RR42_RS30515 at 2220.402 kb on - strand, within RR42_RS30515 at 2220.445 kb on + strand, within RR42_RS30515 at 2220.446 kb on - strand, within RR42_RS30515 at 2220.451 kb on + strand, within RR42_RS30515 at 2220.451 kb on + strand, within RR42_RS30515 at 2220.451 kb on + strand, within RR42_RS30515 at 2220.452 kb on - strand, within RR42_RS30515 at 2220.452 kb on - strand, within RR42_RS30515 at 2220.452 kb on - strand, within RR42_RS30515 at 2220.452 kb on - strand, within RR42_RS30515 at 2220.589 kb on + strand, within RR42_RS30515 at 2220.590 kb on - strand, within RR42_RS30515 at 2220.674 kb on + strand at 2220.738 kb on + strand at 2220.738 kb on + strand at 2220.738 kb on + strand at 2220.738 kb on + strand at 2220.738 kb on + strand at 2220.739 kb on - strand at 2220.739 kb on - strand at 2220.793 kb on - strand at 2220.811 kb on + strand at 2220.946 kb on + strand, within RR42_RS30520 at 2220.946 kb on + strand, within RR42_RS30520 at 2220.946 kb on + strand, within RR42_RS30520 at 2220.947 kb on - strand, within RR42_RS30520 at 2220.947 kb on - strand, within RR42_RS30520 at 2220.947 kb on - strand, within RR42_RS30520 at 2220.947 kb on - strand, within RR42_RS30520 at 2220.971 kb on - strand, within RR42_RS30520 at 2221.000 kb on + strand, within RR42_RS30520 at 2221.013 kb on + strand, within RR42_RS30520 at 2221.014 kb on - strand, within RR42_RS30520 at 2221.057 kb on + strand, within RR42_RS30520 at 2221.058 kb on - strand, within RR42_RS30520 at 2221.136 kb on - strand, within RR42_RS30520 at 2221.141 kb on + strand, within RR42_RS30520 at 2221.141 kb on + strand, within RR42_RS30520 at 2221.142 kb on - strand, within RR42_RS30520 at 2221.258 kb on + strand, within RR42_RS30520 at 2221.461 kb on - strand, within RR42_RS30520 at 2221.461 kb on - strand, within RR42_RS30520 at 2221.575 kb on - strand, within RR42_RS30520 at 2221.660 kb on + strand, within RR42_RS30520
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,219,012 - RR42_RS30505 0.81 +0.9 2,219,319 - +3.0 2,219,559 - RR42_RS30510 0.39 -0.3 2,219,606 + RR42_RS30510 0.47 -0.0 2,219,606 + RR42_RS30510 0.47 -0.9 2,219,735 + RR42_RS30510 0.67 -1.1 2,219,822 + RR42_RS30510 0.80 -0.8 2,219,822 + RR42_RS30510 0.80 -0.5 2,219,822 + RR42_RS30510 0.80 -0.5 2,219,822 + RR42_RS30510 0.80 -0.4 2,219,823 - RR42_RS30510 0.80 +0.2 2,219,937 + -0.2 2,219,938 - -0.9 2,219,938 - +0.2 2,219,939 + +0.8 2,220,089 - RR42_RS30515 0.14 -2.9 2,220,119 - RR42_RS30515 0.18 -0.4 2,220,401 + RR42_RS30515 0.59 +1.7 2,220,402 - RR42_RS30515 0.59 -0.1 2,220,445 + RR42_RS30515 0.65 -0.3 2,220,446 - RR42_RS30515 0.65 -0.4 2,220,451 + RR42_RS30515 0.66 -0.2 2,220,451 + RR42_RS30515 0.66 -0.2 2,220,451 + RR42_RS30515 0.66 +0.0 2,220,452 - RR42_RS30515 0.66 -0.4 2,220,452 - RR42_RS30515 0.66 +0.5 2,220,452 - RR42_RS30515 0.66 -1.1 2,220,452 - RR42_RS30515 0.66 -1.6 2,220,589 + RR42_RS30515 0.86 +0.6 2,220,590 - RR42_RS30515 0.86 -1.0 2,220,674 + -0.2 2,220,738 + -0.8 2,220,738 + +0.0 2,220,738 + -0.8 2,220,738 + -0.0 2,220,738 + -0.0 2,220,739 - -0.4 2,220,739 - +1.8 2,220,793 - -1.4 2,220,811 + +1.6 2,220,946 + RR42_RS30520 0.18 -2.0 2,220,946 + RR42_RS30520 0.18 +1.1 2,220,946 + RR42_RS30520 0.18 -0.2 2,220,947 - RR42_RS30520 0.18 +0.7 2,220,947 - RR42_RS30520 0.18 +0.8 2,220,947 - RR42_RS30520 0.18 +0.9 2,220,947 - RR42_RS30520 0.18 +0.5 2,220,971 - RR42_RS30520 0.21 +1.0 2,221,000 + RR42_RS30520 0.23 +1.6 2,221,013 + RR42_RS30520 0.25 +0.2 2,221,014 - RR42_RS30520 0.25 +0.2 2,221,057 + RR42_RS30520 0.29 +0.7 2,221,058 - RR42_RS30520 0.29 -0.6 2,221,136 - RR42_RS30520 0.36 -0.2 2,221,141 + RR42_RS30520 0.37 -0.3 2,221,141 + RR42_RS30520 0.37 -0.5 2,221,142 - RR42_RS30520 0.37 -2.1 2,221,258 + RR42_RS30520 0.48 -0.4 2,221,461 - RR42_RS30520 0.67 +0.6 2,221,461 - RR42_RS30520 0.67 -0.9 2,221,575 - RR42_RS30520 0.78 -0.3 2,221,660 + RR42_RS30520 0.86 -0.0
Or see this region's nucleotide sequence