Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30300 and RR42_RS30305 overlap by 4 nucleotides RR42_RS30305 and RR42_RS30310 are separated by 464 nucleotides RR42_RS30310 and RR42_RS30315 are separated by 63 nucleotides RR42_RS30315 and RR42_RS30320 overlap by 32 nucleotides
RR42_RS30300: RR42_RS30300 - toxin, at 2,174,868 to 2,175,383
_RS30300
RR42_RS30305: RR42_RS30305 - regulator, at 2,175,380 to 2,175,709
_RS30305
RR42_RS30310: RR42_RS30310 - signal transduction protein, at 2,176,174 to 2,176,602
_RS30310
RR42_RS30315: RR42_RS30315 - hypothetical protein, at 2,176,666 to 2,177,097
_RS30315
RR42_RS30320: RR42_RS30320 - hypothetical protein, at 2,177,066 to 2,178,043
_RS30320
Position (kb)
2176
2177 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2175.254 kb on - strand, within RR42_RS30300 at 2175.601 kb on - strand, within RR42_RS30305 at 2175.831 kb on + strand at 2175.831 kb on + strand at 2175.831 kb on + strand at 2175.832 kb on - strand at 2176.078 kb on - strand at 2176.140 kb on - strand at 2176.148 kb on - strand at 2176.170 kb on - strand at 2176.172 kb on + strand at 2176.172 kb on + strand at 2176.172 kb on + strand at 2176.180 kb on - strand at 2176.180 kb on - strand at 2176.180 kb on - strand at 2176.185 kb on + strand at 2176.185 kb on + strand at 2176.185 kb on + strand at 2176.185 kb on + strand at 2176.364 kb on + strand, within RR42_RS30310 at 2176.386 kb on + strand, within RR42_RS30310 at 2176.600 kb on + strand at 2176.601 kb on - strand at 2176.601 kb on - strand at 2176.601 kb on - strand at 2176.638 kb on + strand at 2176.638 kb on + strand at 2176.639 kb on - strand at 2176.639 kb on - strand at 2176.667 kb on + strand at 2176.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,175,254 - RR42_RS30300 0.75 +0.7 2,175,601 - RR42_RS30305 0.67 +0.5 2,175,831 + +0.4 2,175,831 + -1.6 2,175,831 + -0.3 2,175,832 - -1.4 2,176,078 - +0.6 2,176,140 - +2.2 2,176,148 - -0.3 2,176,170 - +0.4 2,176,172 + -0.5 2,176,172 + -0.3 2,176,172 + -0.0 2,176,180 - -1.4 2,176,180 - -0.4 2,176,180 - -0.3 2,176,185 + +0.4 2,176,185 + +0.2 2,176,185 + -0.6 2,176,185 + -0.8 2,176,364 + RR42_RS30310 0.44 -0.6 2,176,386 + RR42_RS30310 0.49 -1.4 2,176,600 + -0.4 2,176,601 - -0.9 2,176,601 - -1.2 2,176,601 - +0.7 2,176,638 + -1.8 2,176,638 + -0.9 2,176,639 - -0.0 2,176,639 - -1.5 2,176,667 + -0.3 2,176,673 + +0.7
Or see this region's nucleotide sequence