Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30155 and RR42_RS30160 are separated by 144 nucleotides RR42_RS30160 and RR42_RS30165 are separated by 178 nucleotides
RR42_RS30155: RR42_RS30155 - sulfatase, at 2,138,193 to 2,140,001
_RS30155
RR42_RS30160: RR42_RS30160 - sulfatase, at 2,140,146 to 2,141,345
_RS30160
RR42_RS30165: RR42_RS30165 - membrane protein, at 2,141,524 to 2,142,720
_RS30165
Position (kb)
2140
2141
2142 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2139.233 kb on - strand, within RR42_RS30155 at 2139.293 kb on + strand, within RR42_RS30155 at 2139.301 kb on + strand, within RR42_RS30155 at 2139.301 kb on + strand, within RR42_RS30155 at 2139.553 kb on + strand, within RR42_RS30155 at 2139.575 kb on - strand, within RR42_RS30155 at 2139.616 kb on + strand, within RR42_RS30155 at 2139.617 kb on - strand, within RR42_RS30155 at 2139.617 kb on - strand, within RR42_RS30155 at 2139.669 kb on + strand, within RR42_RS30155 at 2139.718 kb on + strand, within RR42_RS30155 at 2139.773 kb on - strand, within RR42_RS30155 at 2139.784 kb on + strand, within RR42_RS30155 at 2139.784 kb on + strand, within RR42_RS30155 at 2139.785 kb on - strand, within RR42_RS30155 at 2139.815 kb on + strand, within RR42_RS30155 at 2139.816 kb on - strand, within RR42_RS30155 at 2139.844 kb on + strand at 2140.055 kb on + strand at 2140.055 kb on + strand at 2140.056 kb on - strand at 2140.076 kb on - strand at 2140.313 kb on - strand, within RR42_RS30160 at 2140.510 kb on + strand, within RR42_RS30160 at 2140.510 kb on + strand, within RR42_RS30160 at 2140.511 kb on - strand, within RR42_RS30160 at 2140.520 kb on - strand, within RR42_RS30160 at 2140.666 kb on + strand, within RR42_RS30160 at 2140.666 kb on + strand, within RR42_RS30160 at 2140.666 kb on + strand, within RR42_RS30160 at 2140.666 kb on + strand, within RR42_RS30160 at 2140.667 kb on - strand, within RR42_RS30160 at 2140.667 kb on - strand, within RR42_RS30160 at 2140.667 kb on - strand, within RR42_RS30160 at 2140.748 kb on - strand, within RR42_RS30160 at 2140.871 kb on - strand, within RR42_RS30160 at 2141.014 kb on + strand, within RR42_RS30160 at 2141.014 kb on + strand, within RR42_RS30160 at 2141.015 kb on - strand, within RR42_RS30160 at 2141.454 kb on - strand at 2141.454 kb on - strand at 2141.507 kb on + strand at 2141.508 kb on - strand at 2141.508 kb on - strand at 2141.526 kb on - strand at 2141.581 kb on + strand at 2141.582 kb on - strand at 2141.645 kb on + strand, within RR42_RS30165 at 2141.646 kb on - strand, within RR42_RS30165 at 2141.646 kb on - strand, within RR42_RS30165 at 2141.660 kb on + strand, within RR42_RS30165 at 2141.660 kb on + strand, within RR42_RS30165 at 2141.774 kb on + strand, within RR42_RS30165 at 2141.774 kb on + strand, within RR42_RS30165 at 2141.774 kb on + strand, within RR42_RS30165 at 2141.775 kb on - strand, within RR42_RS30165 at 2141.775 kb on - strand, within RR42_RS30165 at 2141.775 kb on - strand, within RR42_RS30165 at 2141.775 kb on - strand, within RR42_RS30165 at 2141.778 kb on - strand, within RR42_RS30165 at 2141.924 kb on + strand, within RR42_RS30165 at 2141.924 kb on + strand, within RR42_RS30165 at 2141.925 kb on - strand, within RR42_RS30165 at 2141.925 kb on - strand, within RR42_RS30165 at 2142.029 kb on + strand, within RR42_RS30165 at 2142.029 kb on + strand, within RR42_RS30165 at 2142.030 kb on - strand, within RR42_RS30165 at 2142.054 kb on + strand, within RR42_RS30165 at 2142.055 kb on - strand, within RR42_RS30165 at 2142.143 kb on + strand, within RR42_RS30165 at 2142.144 kb on - strand, within RR42_RS30165 at 2142.182 kb on + strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,139,233 - RR42_RS30155 0.57 +1.3 2,139,293 + RR42_RS30155 0.61 -3.0 2,139,301 + RR42_RS30155 0.61 -0.8 2,139,301 + RR42_RS30155 0.61 +0.2 2,139,553 + RR42_RS30155 0.75 -0.4 2,139,575 - RR42_RS30155 0.76 +0.3 2,139,616 + RR42_RS30155 0.79 +1.2 2,139,617 - RR42_RS30155 0.79 +0.0 2,139,617 - RR42_RS30155 0.79 -1.9 2,139,669 + RR42_RS30155 0.82 +1.2 2,139,718 + RR42_RS30155 0.84 -0.1 2,139,773 - RR42_RS30155 0.87 +0.3 2,139,784 + RR42_RS30155 0.88 +0.6 2,139,784 + RR42_RS30155 0.88 -0.4 2,139,785 - RR42_RS30155 0.88 +1.2 2,139,815 + RR42_RS30155 0.90 -0.3 2,139,816 - RR42_RS30155 0.90 +1.3 2,139,844 + -0.2 2,140,055 + +0.4 2,140,055 + +1.0 2,140,056 - +0.5 2,140,076 - -0.0 2,140,313 - RR42_RS30160 0.14 -1.8 2,140,510 + RR42_RS30160 0.30 +0.7 2,140,510 + RR42_RS30160 0.30 +1.3 2,140,511 - RR42_RS30160 0.30 +0.5 2,140,520 - RR42_RS30160 0.31 -0.6 2,140,666 + RR42_RS30160 0.43 -1.4 2,140,666 + RR42_RS30160 0.43 +0.1 2,140,666 + RR42_RS30160 0.43 -0.4 2,140,666 + RR42_RS30160 0.43 +0.5 2,140,667 - RR42_RS30160 0.43 +0.1 2,140,667 - RR42_RS30160 0.43 -0.6 2,140,667 - RR42_RS30160 0.43 -0.6 2,140,748 - RR42_RS30160 0.50 -0.4 2,140,871 - RR42_RS30160 0.60 -0.2 2,141,014 + RR42_RS30160 0.72 -0.7 2,141,014 + RR42_RS30160 0.72 -1.2 2,141,015 - RR42_RS30160 0.72 +0.9 2,141,454 - +1.2 2,141,454 - +0.3 2,141,507 + +0.4 2,141,508 - -0.2 2,141,508 - -0.0 2,141,526 - +1.2 2,141,581 + +1.6 2,141,582 - +0.4 2,141,645 + RR42_RS30165 0.10 +1.3 2,141,646 - RR42_RS30165 0.10 +0.4 2,141,646 - RR42_RS30165 0.10 -0.3 2,141,660 + RR42_RS30165 0.11 +0.7 2,141,660 + RR42_RS30165 0.11 -0.3 2,141,774 + RR42_RS30165 0.21 -0.6 2,141,774 + RR42_RS30165 0.21 -0.1 2,141,774 + RR42_RS30165 0.21 +0.2 2,141,775 - RR42_RS30165 0.21 +0.2 2,141,775 - RR42_RS30165 0.21 +0.3 2,141,775 - RR42_RS30165 0.21 +0.1 2,141,775 - RR42_RS30165 0.21 +1.1 2,141,778 - RR42_RS30165 0.21 +3.0 2,141,924 + RR42_RS30165 0.33 -0.8 2,141,924 + RR42_RS30165 0.33 +1.4 2,141,925 - RR42_RS30165 0.34 -0.3 2,141,925 - RR42_RS30165 0.34 -0.3 2,142,029 + RR42_RS30165 0.42 +0.1 2,142,029 + RR42_RS30165 0.42 -0.6 2,142,030 - RR42_RS30165 0.42 +0.6 2,142,054 + RR42_RS30165 0.44 -1.1 2,142,055 - RR42_RS30165 0.44 -0.1 2,142,143 + RR42_RS30165 0.52 -0.1 2,142,144 - RR42_RS30165 0.52 -0.4 2,142,182 + RR42_RS30165 0.55 -0.1 2,142,294 - RR42_RS30165 0.64 +0.5 2,142,294 - RR42_RS30165 0.64 +1.6 2,142,294 - RR42_RS30165 0.64 +1.2 2,142,294 - RR42_RS30165 0.64 -0.0
Or see this region's nucleotide sequence