Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29430 and RR42_RS29435 are separated by 103 nucleotides RR42_RS29435 and RR42_RS29440 are separated by 261 nucleotides RR42_RS29440 and RR42_RS29445 are separated by 7 nucleotides
RR42_RS29430: RR42_RS29430 - acyl-CoA synthetase, at 1,957,727 to 1,959,232
_RS29430
RR42_RS29435: RR42_RS29435 - thiopurine S-methyltransferase, at 1,959,336 to 1,959,947
_RS29435
RR42_RS29440: RR42_RS29440 - ABC transporter permease, at 1,960,209 to 1,961,081
_RS29440
RR42_RS29445: RR42_RS29445 - ABC transporter permease, at 1,961,089 to 1,962,165
_RS29445
Position (kb)
1960
1961
1962 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1959.230 kb on + strand at 1959.231 kb on - strand at 1959.231 kb on - strand at 1959.231 kb on - strand at 1959.231 kb on - strand at 1959.238 kb on + strand at 1959.239 kb on - strand at 1959.544 kb on - strand, within RR42_RS29435 at 1959.660 kb on + strand, within RR42_RS29435 at 1959.660 kb on + strand, within RR42_RS29435 at 1959.660 kb on + strand, within RR42_RS29435 at 1959.660 kb on + strand, within RR42_RS29435 at 1959.661 kb on - strand, within RR42_RS29435 at 1959.661 kb on - strand, within RR42_RS29435 at 1959.661 kb on - strand, within RR42_RS29435 at 1959.887 kb on + strand at 1959.888 kb on - strand at 1959.928 kb on + strand at 1959.929 kb on - strand at 1959.989 kb on - strand at 1960.003 kb on - strand at 1960.003 kb on - strand at 1960.003 kb on - strand at 1960.004 kb on + strand at 1960.004 kb on + strand at 1960.005 kb on - strand at 1960.005 kb on - strand at 1960.005 kb on - strand at 1960.005 kb on - strand at 1960.034 kb on + strand at 1960.043 kb on + strand at 1960.044 kb on - strand at 1960.257 kb on + strand at 1960.258 kb on - strand at 1960.258 kb on - strand at 1960.290 kb on + strand at 1960.290 kb on + strand at 1960.290 kb on + strand at 1960.291 kb on - strand at 1960.565 kb on + strand, within RR42_RS29440 at 1960.565 kb on + strand, within RR42_RS29440 at 1960.565 kb on + strand, within RR42_RS29440 at 1960.566 kb on - strand, within RR42_RS29440 at 1960.566 kb on - strand, within RR42_RS29440 at 1960.566 kb on - strand, within RR42_RS29440 at 1960.582 kb on - strand, within RR42_RS29440 at 1960.674 kb on + strand, within RR42_RS29440 at 1960.743 kb on + strand, within RR42_RS29440 at 1960.746 kb on + strand, within RR42_RS29440 at 1960.746 kb on + strand, within RR42_RS29440 at 1960.746 kb on + strand, within RR42_RS29440 at 1960.746 kb on + strand, within RR42_RS29440 at 1960.747 kb on - strand, within RR42_RS29440 at 1960.747 kb on - strand, within RR42_RS29440 at 1960.747 kb on - strand, within RR42_RS29440 at 1960.975 kb on - strand, within RR42_RS29440 at 1961.008 kb on - strand at 1961.008 kb on - strand at 1961.008 kb on - strand at 1961.113 kb on + strand at 1961.151 kb on + strand at 1961.151 kb on + strand at 1961.152 kb on - strand at 1961.162 kb on - strand at 1961.245 kb on + strand, within RR42_RS29445 at 1961.246 kb on - strand, within RR42_RS29445 at 1961.335 kb on + strand, within RR42_RS29445 at 1961.335 kb on + strand, within RR42_RS29445 at 1961.336 kb on - strand, within RR42_RS29445 at 1961.336 kb on - strand, within RR42_RS29445 at 1961.337 kb on + strand, within RR42_RS29445 at 1961.337 kb on + strand, within RR42_RS29445 at 1961.338 kb on - strand, within RR42_RS29445 at 1961.338 kb on - strand, within RR42_RS29445 at 1961.338 kb on - strand, within RR42_RS29445 at 1961.458 kb on + strand, within RR42_RS29445 at 1961.458 kb on + strand, within RR42_RS29445 at 1961.458 kb on + strand, within RR42_RS29445 at 1961.459 kb on - strand, within RR42_RS29445 at 1961.459 kb on - strand, within RR42_RS29445 at 1961.459 kb on - strand, within RR42_RS29445 at 1961.459 kb on - strand, within RR42_RS29445 at 1961.459 kb on - strand, within RR42_RS29445 at 1961.459 kb on - strand, within RR42_RS29445 at 1961.575 kb on + strand, within RR42_RS29445 at 1961.575 kb on + strand, within RR42_RS29445 at 1961.576 kb on - strand, within RR42_RS29445 at 1961.576 kb on - strand, within RR42_RS29445 at 1961.576 kb on - strand, within RR42_RS29445 at 1961.602 kb on + strand, within RR42_RS29445 at 1961.602 kb on + strand, within RR42_RS29445 at 1961.603 kb on - strand, within RR42_RS29445 at 1961.603 kb on - strand, within RR42_RS29445 at 1961.605 kb on + strand, within RR42_RS29445 at 1961.605 kb on + strand, within RR42_RS29445 at 1961.606 kb on - strand, within RR42_RS29445 at 1961.606 kb on - strand, within RR42_RS29445 at 1961.606 kb on - strand, within RR42_RS29445 at 1961.797 kb on + strand, within RR42_RS29445 at 1961.797 kb on + strand, within RR42_RS29445 at 1961.816 kb on - strand, within RR42_RS29445 at 1961.816 kb on - strand, within RR42_RS29445 at 1961.816 kb on - strand, within RR42_RS29445 at 1961.817 kb on + strand, within RR42_RS29445 at 1961.818 kb on - strand, within RR42_RS29445 at 1961.818 kb on - strand, within RR42_RS29445 at 1962.010 kb on + strand, within RR42_RS29445
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,959,230 + -0.9 1,959,231 - +0.6 1,959,231 - +2.6 1,959,231 - -0.1 1,959,231 - -0.4 1,959,238 + -0.3 1,959,239 - -0.6 1,959,544 - RR42_RS29435 0.34 -1.4 1,959,660 + RR42_RS29435 0.53 +0.7 1,959,660 + RR42_RS29435 0.53 -0.9 1,959,660 + RR42_RS29435 0.53 -0.1 1,959,660 + RR42_RS29435 0.53 -0.7 1,959,661 - RR42_RS29435 0.53 -0.7 1,959,661 - RR42_RS29435 0.53 -0.7 1,959,661 - RR42_RS29435 0.53 -0.2 1,959,887 + +0.2 1,959,888 - -0.4 1,959,928 + -1.7 1,959,929 - +0.9 1,959,989 - -0.9 1,960,003 - -0.0 1,960,003 - +0.4 1,960,003 - -0.8 1,960,004 + +1.3 1,960,004 + +1.1 1,960,005 - -1.9 1,960,005 - -0.6 1,960,005 - +1.0 1,960,005 - -1.4 1,960,034 + +0.2 1,960,043 + +1.1 1,960,044 - -0.8 1,960,257 + +0.2 1,960,258 - -0.2 1,960,258 - +0.2 1,960,290 + +1.1 1,960,290 + -0.0 1,960,290 + +0.7 1,960,291 - -0.9 1,960,565 + RR42_RS29440 0.41 -0.3 1,960,565 + RR42_RS29440 0.41 -1.9 1,960,565 + RR42_RS29440 0.41 +1.1 1,960,566 - RR42_RS29440 0.41 +0.0 1,960,566 - RR42_RS29440 0.41 +0.0 1,960,566 - RR42_RS29440 0.41 -0.1 1,960,582 - RR42_RS29440 0.43 -0.1 1,960,674 + RR42_RS29440 0.53 -0.8 1,960,743 + RR42_RS29440 0.61 +0.7 1,960,746 + RR42_RS29440 0.62 -0.9 1,960,746 + RR42_RS29440 0.62 -1.3 1,960,746 + RR42_RS29440 0.62 -0.3 1,960,746 + RR42_RS29440 0.62 -0.4 1,960,747 - RR42_RS29440 0.62 +1.6 1,960,747 - RR42_RS29440 0.62 -0.8 1,960,747 - RR42_RS29440 0.62 -0.1 1,960,975 - RR42_RS29440 0.88 -0.7 1,961,008 - -1.9 1,961,008 - -0.3 1,961,008 - -0.7 1,961,113 + +0.3 1,961,151 + +0.7 1,961,151 + -0.7 1,961,152 - +1.0 1,961,162 - -1.0 1,961,245 + RR42_RS29445 0.14 -0.4 1,961,246 - RR42_RS29445 0.15 -0.1 1,961,335 + RR42_RS29445 0.23 -0.4 1,961,335 + RR42_RS29445 0.23 -1.5 1,961,336 - RR42_RS29445 0.23 +1.0 1,961,336 - RR42_RS29445 0.23 -0.3 1,961,337 + RR42_RS29445 0.23 -0.4 1,961,337 + RR42_RS29445 0.23 -1.1 1,961,338 - RR42_RS29445 0.23 -0.9 1,961,338 - RR42_RS29445 0.23 +0.5 1,961,338 - RR42_RS29445 0.23 +1.8 1,961,458 + RR42_RS29445 0.34 -0.4 1,961,458 + RR42_RS29445 0.34 +0.3 1,961,458 + RR42_RS29445 0.34 +0.1 1,961,459 - RR42_RS29445 0.34 +1.0 1,961,459 - RR42_RS29445 0.34 -1.0 1,961,459 - RR42_RS29445 0.34 +1.1 1,961,459 - RR42_RS29445 0.34 +0.2 1,961,459 - RR42_RS29445 0.34 -0.8 1,961,459 - RR42_RS29445 0.34 +0.4 1,961,575 + RR42_RS29445 0.45 -0.4 1,961,575 + RR42_RS29445 0.45 +0.1 1,961,576 - RR42_RS29445 0.45 -0.9 1,961,576 - RR42_RS29445 0.45 -0.3 1,961,576 - RR42_RS29445 0.45 -0.7 1,961,602 + RR42_RS29445 0.48 +0.2 1,961,602 + RR42_RS29445 0.48 -1.8 1,961,603 - RR42_RS29445 0.48 +0.1 1,961,603 - RR42_RS29445 0.48 +0.4 1,961,605 + RR42_RS29445 0.48 +1.2 1,961,605 + RR42_RS29445 0.48 +0.4 1,961,606 - RR42_RS29445 0.48 -0.7 1,961,606 - RR42_RS29445 0.48 -1.4 1,961,606 - RR42_RS29445 0.48 -0.6 1,961,797 + RR42_RS29445 0.66 -1.0 1,961,797 + RR42_RS29445 0.66 -0.5 1,961,816 - RR42_RS29445 0.68 -0.7 1,961,816 - RR42_RS29445 0.68 -0.0 1,961,816 - RR42_RS29445 0.68 -0.0 1,961,817 + RR42_RS29445 0.68 -1.5 1,961,818 - RR42_RS29445 0.68 +0.2 1,961,818 - RR42_RS29445 0.68 -0.3 1,962,010 + RR42_RS29445 0.86 -0.3
Or see this region's nucleotide sequence