Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29275 and RR42_RS29280 are separated by 89 nucleotides RR42_RS29280 and RR42_RS29285 are separated by 34 nucleotides RR42_RS29285 and RR42_RS29290 are separated by 95 nucleotides RR42_RS29290 and RR42_RS29295 are separated by 159 nucleotides
RR42_RS29275: RR42_RS29275 - permease, at 1,921,503 to 1,922,312
_RS29275
RR42_RS29280: RR42_RS29280 - hypothetical protein, at 1,922,402 to 1,922,581
_RS29280
RR42_RS29285: RR42_RS29285 - hypothetical protein, at 1,922,616 to 1,923,041
_RS29285
RR42_RS29290: RR42_RS29290 - diguanylate cyclase, at 1,923,137 to 1,924,108
_RS29290
RR42_RS29295: RR42_RS29295 - hypothetical protein, at 1,924,268 to 1,924,684
_RS29295
Position (kb)
1923
1924
1925 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1922.157 kb on + strand, within RR42_RS29275 at 1922.157 kb on + strand, within RR42_RS29275 at 1922.157 kb on + strand, within RR42_RS29275 at 1922.186 kb on - strand, within RR42_RS29275 at 1922.265 kb on + strand at 1922.266 kb on - strand at 1922.310 kb on + strand at 1922.310 kb on + strand at 1922.364 kb on + strand at 1922.364 kb on + strand at 1922.364 kb on + strand at 1922.365 kb on - strand at 1922.365 kb on - strand at 1922.365 kb on - strand at 1922.365 kb on - strand at 1922.365 kb on - strand at 1922.365 kb on - strand at 1922.470 kb on + strand, within RR42_RS29280 at 1922.618 kb on - strand at 1922.704 kb on + strand, within RR42_RS29285 at 1922.857 kb on + strand, within RR42_RS29285 at 1922.857 kb on + strand, within RR42_RS29285 at 1922.857 kb on + strand, within RR42_RS29285 at 1922.858 kb on - strand, within RR42_RS29285 at 1922.920 kb on + strand, within RR42_RS29285 at 1923.035 kb on + strand at 1923.080 kb on + strand at 1923.080 kb on + strand at 1923.081 kb on - strand at 1923.082 kb on + strand at 1923.082 kb on + strand at 1923.082 kb on + strand at 1923.083 kb on - strand at 1923.083 kb on - strand at 1923.083 kb on - strand at 1923.083 kb on - strand at 1923.174 kb on + strand at 1923.174 kb on + strand at 1923.174 kb on + strand at 1923.174 kb on + strand at 1923.175 kb on - strand at 1923.175 kb on - strand at 1923.175 kb on - strand at 1923.175 kb on - strand at 1923.175 kb on - strand at 1923.756 kb on - strand, within RR42_RS29290 at 1923.771 kb on + strand, within RR42_RS29290 at 1924.045 kb on + strand at 1924.046 kb on - strand at 1924.120 kb on + strand at 1924.121 kb on - strand at 1924.133 kb on + strand at 1924.185 kb on + strand at 1924.186 kb on - strand at 1924.209 kb on + strand at 1924.209 kb on + strand at 1924.210 kb on - strand at 1924.362 kb on + strand, within RR42_RS29295 at 1924.362 kb on + strand, within RR42_RS29295 at 1924.363 kb on - strand, within RR42_RS29295 at 1924.534 kb on + strand, within RR42_RS29295 at 1924.616 kb on + strand, within RR42_RS29295 at 1924.616 kb on + strand, within RR42_RS29295 at 1924.617 kb on - strand, within RR42_RS29295 at 1924.737 kb on + strand at 1924.737 kb on + strand at 1924.738 kb on - strand at 1924.769 kb on - strand at 1924.769 kb on - strand at 1924.800 kb on + strand at 1924.800 kb on + strand at 1924.801 kb on - strand at 1924.880 kb on + strand at 1924.881 kb on - strand at 1924.884 kb on + strand at 1924.907 kb on - strand at 1924.915 kb on + strand at 1925.084 kb on + strand at 1925.085 kb on - strand at 1925.085 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,922,157 + RR42_RS29275 0.81 +0.3 1,922,157 + RR42_RS29275 0.81 +0.6 1,922,157 + RR42_RS29275 0.81 +0.2 1,922,186 - RR42_RS29275 0.84 +0.1 1,922,265 + +1.0 1,922,266 - -1.0 1,922,310 + -0.7 1,922,310 + +0.6 1,922,364 + -0.0 1,922,364 + -0.1 1,922,364 + -0.7 1,922,365 - -0.2 1,922,365 - -0.4 1,922,365 - +2.6 1,922,365 - +0.1 1,922,365 - +0.4 1,922,365 - -0.5 1,922,470 + RR42_RS29280 0.38 -2.8 1,922,618 - +2.8 1,922,704 + RR42_RS29285 0.21 +1.0 1,922,857 + RR42_RS29285 0.57 +0.6 1,922,857 + RR42_RS29285 0.57 -0.8 1,922,857 + RR42_RS29285 0.57 -0.6 1,922,858 - RR42_RS29285 0.57 +0.6 1,922,920 + RR42_RS29285 0.71 -2.0 1,923,035 + +0.9 1,923,080 + +1.2 1,923,080 + +0.7 1,923,081 - -0.2 1,923,082 + -0.4 1,923,082 + -0.7 1,923,082 + +0.2 1,923,083 - +2.0 1,923,083 - -0.6 1,923,083 - +0.0 1,923,083 - -0.8 1,923,174 + -1.4 1,923,174 + +0.5 1,923,174 + +0.6 1,923,174 + +2.6 1,923,175 - -0.3 1,923,175 - -0.1 1,923,175 - +1.6 1,923,175 - -0.2 1,923,175 - +2.2 1,923,756 - RR42_RS29290 0.64 +0.3 1,923,771 + RR42_RS29290 0.65 -0.0 1,924,045 + -2.0 1,924,046 - -0.3 1,924,120 + +0.4 1,924,121 - +0.5 1,924,133 + +0.8 1,924,185 + -2.1 1,924,186 - -1.2 1,924,209 + -0.6 1,924,209 + -1.0 1,924,210 - +0.5 1,924,362 + RR42_RS29295 0.23 -0.6 1,924,362 + RR42_RS29295 0.23 +0.6 1,924,363 - RR42_RS29295 0.23 +0.8 1,924,534 + RR42_RS29295 0.64 -0.6 1,924,616 + RR42_RS29295 0.83 +0.6 1,924,616 + RR42_RS29295 0.83 +1.6 1,924,617 - RR42_RS29295 0.84 +0.0 1,924,737 + -0.6 1,924,737 + -1.6 1,924,738 - -0.8 1,924,769 - -1.2 1,924,769 - +1.4 1,924,800 + -0.6 1,924,800 + -0.6 1,924,801 - +0.6 1,924,880 + -0.9 1,924,881 - -1.2 1,924,884 + -0.7 1,924,907 - -0.2 1,924,915 + +0.6 1,925,084 + -1.1 1,925,085 - +0.8 1,925,085 - +1.1
Or see this region's nucleotide sequence