Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28725 and RR42_RS28730 overlap by 4 nucleotides RR42_RS28730 and RR42_RS28735 overlap by 4 nucleotides RR42_RS28735 and RR42_RS28740 overlap by 8 nucleotides
RR42_RS28725: RR42_RS28725 - ABC transporter permease, at 1,795,839 to 1,796,705
_RS28725
RR42_RS28730: RR42_RS28730 - ABC transporter permease, at 1,796,702 to 1,797,718
_RS28730
RR42_RS28735: RR42_RS28735 - hemolysin III, at 1,797,715 to 1,798,467
_RS28735
RR42_RS28740: RR42_RS28740 - ABC transporter ATP-binding protein, at 1,798,460 to 1,799,161
_RS28740
Position (kb)
1796
1797
1798 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1795.881 kb on + strand at 1795.882 kb on - strand at 1795.882 kb on - strand at 1795.882 kb on - strand at 1795.909 kb on + strand at 1796.041 kb on + strand, within RR42_RS28725 at 1796.175 kb on + strand, within RR42_RS28725 at 1796.175 kb on + strand, within RR42_RS28725 at 1796.175 kb on + strand, within RR42_RS28725 at 1796.176 kb on - strand, within RR42_RS28725 at 1796.176 kb on - strand, within RR42_RS28725 at 1796.176 kb on - strand, within RR42_RS28725 at 1796.408 kb on - strand, within RR42_RS28725 at 1796.505 kb on + strand, within RR42_RS28725 at 1796.505 kb on + strand, within RR42_RS28725 at 1796.505 kb on + strand, within RR42_RS28725 at 1796.505 kb on + strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.514 kb on + strand, within RR42_RS28725 at 1796.514 kb on + strand, within RR42_RS28725 at 1796.514 kb on + strand, within RR42_RS28725 at 1796.515 kb on - strand, within RR42_RS28725 at 1796.515 kb on - strand, within RR42_RS28725 at 1796.515 kb on - strand, within RR42_RS28725 at 1796.610 kb on + strand, within RR42_RS28725 at 1796.610 kb on + strand, within RR42_RS28725 at 1796.610 kb on + strand, within RR42_RS28725 at 1796.610 kb on + strand, within RR42_RS28725 at 1796.611 kb on - strand, within RR42_RS28725 at 1796.611 kb on - strand, within RR42_RS28725 at 1796.611 kb on - strand, within RR42_RS28725 at 1796.613 kb on + strand, within RR42_RS28725 at 1796.613 kb on + strand, within RR42_RS28725 at 1796.613 kb on + strand, within RR42_RS28725 at 1796.614 kb on - strand, within RR42_RS28725 at 1796.643 kb on + strand at 1796.643 kb on + strand at 1796.643 kb on + strand at 1796.643 kb on + strand at 1796.644 kb on - strand at 1796.644 kb on - strand at 1796.644 kb on - strand at 1796.644 kb on - strand at 1796.644 kb on - strand at 1796.951 kb on + strand, within RR42_RS28730 at 1796.951 kb on + strand, within RR42_RS28730 at 1796.951 kb on + strand, within RR42_RS28730 at 1796.952 kb on - strand, within RR42_RS28730 at 1796.952 kb on - strand, within RR42_RS28730 at 1796.952 kb on - strand, within RR42_RS28730 at 1797.230 kb on + strand, within RR42_RS28730 at 1797.524 kb on + strand, within RR42_RS28730 at 1797.524 kb on + strand, within RR42_RS28730 at 1797.524 kb on + strand, within RR42_RS28730 at 1797.524 kb on + strand, within RR42_RS28730 at 1797.525 kb on - strand, within RR42_RS28730 at 1797.593 kb on + strand, within RR42_RS28730 at 1797.594 kb on - strand, within RR42_RS28730 at 1797.732 kb on + strand at 1797.733 kb on - strand at 1797.934 kb on + strand, within RR42_RS28735 at 1797.935 kb on - strand, within RR42_RS28735 at 1797.967 kb on + strand, within RR42_RS28735 at 1797.968 kb on - strand, within RR42_RS28735 at 1797.968 kb on - strand, within RR42_RS28735 at 1798.341 kb on - strand, within RR42_RS28735 at 1798.341 kb on - strand, within RR42_RS28735 at 1798.439 kb on + strand at 1798.439 kb on + strand at 1798.441 kb on + strand at 1798.442 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,795,881 + -0.2 1,795,882 - -0.8 1,795,882 - -0.7 1,795,882 - +0.5 1,795,909 + +0.6 1,796,041 + RR42_RS28725 0.23 -1.2 1,796,175 + RR42_RS28725 0.39 -1.4 1,796,175 + RR42_RS28725 0.39 +0.3 1,796,175 + RR42_RS28725 0.39 -0.4 1,796,176 - RR42_RS28725 0.39 +0.7 1,796,176 - RR42_RS28725 0.39 +0.8 1,796,176 - RR42_RS28725 0.39 -1.5 1,796,408 - RR42_RS28725 0.66 +0.3 1,796,505 + RR42_RS28725 0.77 +0.5 1,796,505 + RR42_RS28725 0.77 -1.6 1,796,505 + RR42_RS28725 0.77 -0.5 1,796,505 + RR42_RS28725 0.77 +1.0 1,796,506 - RR42_RS28725 0.77 -1.0 1,796,506 - RR42_RS28725 0.77 -0.7 1,796,506 - RR42_RS28725 0.77 -1.3 1,796,506 - RR42_RS28725 0.77 +0.4 1,796,506 - RR42_RS28725 0.77 +0.4 1,796,514 + RR42_RS28725 0.78 -0.6 1,796,514 + RR42_RS28725 0.78 +0.3 1,796,514 + RR42_RS28725 0.78 -0.7 1,796,515 - RR42_RS28725 0.78 +0.3 1,796,515 - RR42_RS28725 0.78 -1.9 1,796,515 - RR42_RS28725 0.78 +0.6 1,796,610 + RR42_RS28725 0.89 +1.3 1,796,610 + RR42_RS28725 0.89 +1.1 1,796,610 + RR42_RS28725 0.89 -1.0 1,796,610 + RR42_RS28725 0.89 -0.2 1,796,611 - RR42_RS28725 0.89 -1.3 1,796,611 - RR42_RS28725 0.89 +1.0 1,796,611 - RR42_RS28725 0.89 +0.0 1,796,613 + RR42_RS28725 0.89 -0.2 1,796,613 + RR42_RS28725 0.89 +2.0 1,796,613 + RR42_RS28725 0.89 -0.3 1,796,614 - RR42_RS28725 0.89 -1.2 1,796,643 + -1.1 1,796,643 + +0.2 1,796,643 + -0.0 1,796,643 + -0.5 1,796,644 - +0.2 1,796,644 - +0.2 1,796,644 - +1.0 1,796,644 - -0.7 1,796,644 - +0.3 1,796,951 + RR42_RS28730 0.24 -1.2 1,796,951 + RR42_RS28730 0.24 -0.8 1,796,951 + RR42_RS28730 0.24 -0.6 1,796,952 - RR42_RS28730 0.25 -1.2 1,796,952 - RR42_RS28730 0.25 +0.6 1,796,952 - RR42_RS28730 0.25 +0.3 1,797,230 + RR42_RS28730 0.52 -0.2 1,797,524 + RR42_RS28730 0.81 -1.4 1,797,524 + RR42_RS28730 0.81 -0.7 1,797,524 + RR42_RS28730 0.81 -0.3 1,797,524 + RR42_RS28730 0.81 -1.3 1,797,525 - RR42_RS28730 0.81 -0.0 1,797,593 + RR42_RS28730 0.88 -1.2 1,797,594 - RR42_RS28730 0.88 -1.9 1,797,732 + +1.5 1,797,733 - -2.0 1,797,934 + RR42_RS28735 0.29 +0.7 1,797,935 - RR42_RS28735 0.29 -1.0 1,797,967 + RR42_RS28735 0.33 -0.3 1,797,968 - RR42_RS28735 0.34 -1.3 1,797,968 - RR42_RS28735 0.34 -0.5 1,798,341 - RR42_RS28735 0.83 -1.1 1,798,341 - RR42_RS28735 0.83 -0.2 1,798,439 + -0.0 1,798,439 + -0.2 1,798,441 + -0.5 1,798,442 - -2.7
Or see this region's nucleotide sequence