Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28415 and RR42_RS28420 are separated by 2 nucleotides RR42_RS28420 and RR42_RS28425 are separated by 15 nucleotides RR42_RS28425 and RR42_RS28430 are separated by 15 nucleotides RR42_RS28430 and RR42_RS28435 are separated by 191 nucleotides
RR42_RS28415: RR42_RS28415 - diguanylate cyclase, at 1,735,172 to 1,735,834
_RS28415
RR42_RS28420: RR42_RS28420 - 4-hydroxy-4-methyl-2-oxoglutarate aldolase, at 1,735,837 to 1,736,499
_RS28420
RR42_RS28425: RR42_RS28425 - 3-hydroxybutyryl-CoA dehydratase, at 1,736,515 to 1,737,285
_RS28425
RR42_RS28430: RR42_RS28430 - phenylacetic acid degradation protein, at 1,737,301 to 1,737,723
_RS28430
RR42_RS28435: RR42_RS28435 - amidase, at 1,737,915 to 1,739,441
_RS28435
Position (kb)
1736
1737
1738 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1735.533 kb on + strand, within RR42_RS28415 at 1735.533 kb on + strand, within RR42_RS28415 at 1735.608 kb on + strand, within RR42_RS28415 at 1735.609 kb on - strand, within RR42_RS28415 at 1736.048 kb on + strand, within RR42_RS28420 at 1736.048 kb on + strand, within RR42_RS28420 at 1736.049 kb on - strand, within RR42_RS28420 at 1736.049 kb on - strand, within RR42_RS28420 at 1736.049 kb on - strand, within RR42_RS28420 at 1736.278 kb on + strand, within RR42_RS28420 at 1736.299 kb on - strand, within RR42_RS28420 at 1736.369 kb on + strand, within RR42_RS28420 at 1736.370 kb on - strand, within RR42_RS28420 at 1736.405 kb on + strand, within RR42_RS28420 at 1736.405 kb on + strand, within RR42_RS28420 at 1736.485 kb on + strand at 1736.578 kb on - strand at 1736.929 kb on + strand, within RR42_RS28425 at 1736.950 kb on + strand, within RR42_RS28425 at 1736.951 kb on - strand, within RR42_RS28425 at 1737.317 kb on - strand at 1737.342 kb on - strand at 1737.697 kb on - strand at 1737.697 kb on - strand at 1738.091 kb on + strand, within RR42_RS28435 at 1738.091 kb on + strand, within RR42_RS28435 at 1738.091 kb on + strand, within RR42_RS28435 at 1738.092 kb on - strand, within RR42_RS28435 at 1738.092 kb on - strand, within RR42_RS28435 at 1738.201 kb on + strand, within RR42_RS28435 at 1738.201 kb on + strand, within RR42_RS28435 at 1738.202 kb on - strand, within RR42_RS28435 at 1738.202 kb on - strand, within RR42_RS28435 at 1738.202 kb on - strand, within RR42_RS28435 at 1738.202 kb on - strand, within RR42_RS28435 at 1738.225 kb on + strand, within RR42_RS28435 at 1738.225 kb on + strand, within RR42_RS28435
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,735,533 + RR42_RS28415 0.54 -1.1 1,735,533 + RR42_RS28415 0.54 -0.8 1,735,608 + RR42_RS28415 0.66 +1.3 1,735,609 - RR42_RS28415 0.66 +0.8 1,736,048 + RR42_RS28420 0.32 +0.3 1,736,048 + RR42_RS28420 0.32 -0.3 1,736,049 - RR42_RS28420 0.32 +0.7 1,736,049 - RR42_RS28420 0.32 -1.1 1,736,049 - RR42_RS28420 0.32 +0.8 1,736,278 + RR42_RS28420 0.67 -0.1 1,736,299 - RR42_RS28420 0.70 -1.4 1,736,369 + RR42_RS28420 0.80 +0.3 1,736,370 - RR42_RS28420 0.80 -0.2 1,736,405 + RR42_RS28420 0.86 +2.9 1,736,405 + RR42_RS28420 0.86 -0.5 1,736,485 + +0.9 1,736,578 - +0.9 1,736,929 + RR42_RS28425 0.54 -1.0 1,736,950 + RR42_RS28425 0.56 -1.6 1,736,951 - RR42_RS28425 0.57 -0.4 1,737,317 - -0.9 1,737,342 - -1.2 1,737,697 - +1.2 1,737,697 - +0.0 1,738,091 + RR42_RS28435 0.12 +0.6 1,738,091 + RR42_RS28435 0.12 +3.2 1,738,091 + RR42_RS28435 0.12 +0.1 1,738,092 - RR42_RS28435 0.12 +0.9 1,738,092 - RR42_RS28435 0.12 +0.6 1,738,201 + RR42_RS28435 0.19 +0.1 1,738,201 + RR42_RS28435 0.19 +0.7 1,738,202 - RR42_RS28435 0.19 -0.2 1,738,202 - RR42_RS28435 0.19 -0.8 1,738,202 - RR42_RS28435 0.19 +0.6 1,738,202 - RR42_RS28435 0.19 +0.4 1,738,225 + RR42_RS28435 0.20 +0.4 1,738,225 + RR42_RS28435 0.20 +0.2
Or see this region's nucleotide sequence