Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28320 and RR42_RS28325 are separated by 223 nucleotides RR42_RS28325 and RR42_RS28330 are separated by 8 nucleotides RR42_RS28330 and RR42_RS28335 are separated by 52 nucleotides
RR42_RS28320: RR42_RS28320 - porin, at 1,714,297 to 1,715,367
_RS28320
RR42_RS28325: RR42_RS28325 - 3-hydroxyacyl-CoA dehydrogenase, at 1,715,591 to 1,716,958
_RS28325
RR42_RS28330: RR42_RS28330 - enoyl-CoA hydratase, at 1,716,967 to 1,717,764
_RS28330
RR42_RS28335: RR42_RS28335 - ferredoxin, at 1,717,817 to 1,718,785
_RS28335
Position (kb)
1715
1716
1717 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1714.727 kb on + strand, within RR42_RS28320 at 1714.764 kb on - strand, within RR42_RS28320 at 1714.797 kb on - strand, within RR42_RS28320 at 1714.814 kb on + strand, within RR42_RS28320 at 1714.898 kb on + strand, within RR42_RS28320 at 1714.898 kb on + strand, within RR42_RS28320 at 1714.899 kb on - strand, within RR42_RS28320 at 1714.955 kb on + strand, within RR42_RS28320 at 1714.955 kb on + strand, within RR42_RS28320 at 1714.982 kb on + strand, within RR42_RS28320 at 1714.982 kb on + strand, within RR42_RS28320 at 1714.983 kb on - strand, within RR42_RS28320 at 1714.983 kb on - strand, within RR42_RS28320 at 1715.012 kb on + strand, within RR42_RS28320 at 1715.012 kb on + strand, within RR42_RS28320 at 1715.013 kb on - strand, within RR42_RS28320 at 1715.029 kb on + strand, within RR42_RS28320 at 1715.029 kb on + strand, within RR42_RS28320 at 1715.236 kb on - strand, within RR42_RS28320 at 1715.356 kb on - strand at 1715.417 kb on - strand at 1715.514 kb on - strand at 1715.514 kb on - strand at 1715.607 kb on - strand at 1715.706 kb on + strand at 1715.706 kb on + strand at 1715.736 kb on + strand, within RR42_RS28325 at 1715.737 kb on - strand, within RR42_RS28325 at 1715.739 kb on + strand, within RR42_RS28325 at 1715.739 kb on + strand, within RR42_RS28325 at 1715.740 kb on - strand, within RR42_RS28325 at 1715.784 kb on + strand, within RR42_RS28325 at 1715.784 kb on + strand, within RR42_RS28325 at 1715.785 kb on - strand, within RR42_RS28325 at 1715.785 kb on - strand, within RR42_RS28325 at 1716.157 kb on - strand, within RR42_RS28325 at 1716.195 kb on - strand, within RR42_RS28325 at 1716.195 kb on - strand, within RR42_RS28325 at 1716.234 kb on + strand, within RR42_RS28325 at 1716.410 kb on + strand, within RR42_RS28325 at 1716.410 kb on + strand, within RR42_RS28325 at 1716.522 kb on - strand, within RR42_RS28325 at 1716.592 kb on + strand, within RR42_RS28325 at 1716.593 kb on - strand, within RR42_RS28325 at 1716.672 kb on + strand, within RR42_RS28325 at 1716.673 kb on - strand, within RR42_RS28325 at 1716.949 kb on + strand at 1716.950 kb on - strand at 1716.950 kb on - strand at 1717.230 kb on + strand, within RR42_RS28330 at 1717.258 kb on - strand, within RR42_RS28330 at 1717.743 kb on - strand at 1717.764 kb on + strand at 1717.764 kb on + strand at 1717.818 kb on + strand at 1717.819 kb on - strand at 1717.943 kb on + strand, within RR42_RS28335
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,714,727 + RR42_RS28320 0.40 -0.2 1,714,764 - RR42_RS28320 0.44 -1.2 1,714,797 - RR42_RS28320 0.47 +0.2 1,714,814 + RR42_RS28320 0.48 -0.4 1,714,898 + RR42_RS28320 0.56 -0.3 1,714,898 + RR42_RS28320 0.56 -1.9 1,714,899 - RR42_RS28320 0.56 -0.1 1,714,955 + RR42_RS28320 0.61 -0.5 1,714,955 + RR42_RS28320 0.61 +1.6 1,714,982 + RR42_RS28320 0.64 -0.6 1,714,982 + RR42_RS28320 0.64 +0.3 1,714,983 - RR42_RS28320 0.64 -0.9 1,714,983 - RR42_RS28320 0.64 -0.2 1,715,012 + RR42_RS28320 0.67 -0.7 1,715,012 + RR42_RS28320 0.67 +0.3 1,715,013 - RR42_RS28320 0.67 -0.5 1,715,029 + RR42_RS28320 0.68 -0.2 1,715,029 + RR42_RS28320 0.68 +2.0 1,715,236 - RR42_RS28320 0.88 +0.6 1,715,356 - +0.1 1,715,417 - -1.0 1,715,514 - +0.0 1,715,514 - +2.0 1,715,607 - -0.2 1,715,706 + +0.8 1,715,706 + +0.1 1,715,736 + RR42_RS28325 0.11 -0.2 1,715,737 - RR42_RS28325 0.11 +0.7 1,715,739 + RR42_RS28325 0.11 +0.3 1,715,739 + RR42_RS28325 0.11 -1.2 1,715,740 - RR42_RS28325 0.11 -1.2 1,715,784 + RR42_RS28325 0.14 -0.7 1,715,784 + RR42_RS28325 0.14 -0.6 1,715,785 - RR42_RS28325 0.14 +0.0 1,715,785 - RR42_RS28325 0.14 -0.8 1,716,157 - RR42_RS28325 0.41 +0.7 1,716,195 - RR42_RS28325 0.44 +0.5 1,716,195 - RR42_RS28325 0.44 -1.6 1,716,234 + RR42_RS28325 0.47 -0.1 1,716,410 + RR42_RS28325 0.60 -1.7 1,716,410 + RR42_RS28325 0.60 -1.1 1,716,522 - RR42_RS28325 0.68 +1.4 1,716,592 + RR42_RS28325 0.73 -0.5 1,716,593 - RR42_RS28325 0.73 -1.8 1,716,672 + RR42_RS28325 0.79 -1.5 1,716,673 - RR42_RS28325 0.79 +1.2 1,716,949 + -1.3 1,716,950 - -1.4 1,716,950 - +0.6 1,717,230 + RR42_RS28330 0.33 +0.4 1,717,258 - RR42_RS28330 0.36 +0.7 1,717,743 - -0.9 1,717,764 + +0.2 1,717,764 + -2.2 1,717,818 + -1.2 1,717,819 - +0.7 1,717,943 + RR42_RS28335 0.13 -1.0
Or see this region's nucleotide sequence