Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28195 and RR42_RS28200 overlap by 8 nucleotides RR42_RS28200 and RR42_RS28205 are separated by 168 nucleotides RR42_RS28205 and RR42_RS28210 are separated by 54 nucleotides
RR42_RS28195: RR42_RS28195 - SAM-dependent methyltransferase, at 1,682,019 to 1,682,897
_RS28195
RR42_RS28200: RR42_RS28200 - membrane protein, at 1,682,890 to 1,683,411
_RS28200
RR42_RS28205: RR42_RS28205 - hypothetical protein, at 1,683,580 to 1,685,718
_RS28205
RR42_RS28210: RR42_RS28210 - LysR family transcriptional regulator, at 1,685,773 to 1,686,699
_RS28210
Position (kb)
1683
1684
1685
1686 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1682.701 kb on - strand, within RR42_RS28195 at 1682.798 kb on + strand, within RR42_RS28195 at 1682.799 kb on + strand, within RR42_RS28195 at 1682.799 kb on + strand, within RR42_RS28195 at 1682.908 kb on + strand at 1682.909 kb on - strand at 1683.121 kb on + strand, within RR42_RS28200 at 1683.156 kb on - strand, within RR42_RS28200 at 1683.285 kb on + strand, within RR42_RS28200 at 1683.285 kb on + strand, within RR42_RS28200 at 1683.286 kb on - strand, within RR42_RS28200 at 1683.315 kb on + strand, within RR42_RS28200 at 1683.316 kb on - strand, within RR42_RS28200 at 1683.352 kb on + strand, within RR42_RS28200 at 1683.503 kb on + strand at 1683.504 kb on - strand at 1683.508 kb on + strand at 1683.510 kb on + strand at 1683.511 kb on - strand at 1683.549 kb on + strand at 1683.580 kb on - strand at 1683.688 kb on - strand at 1683.751 kb on + strand at 1683.751 kb on + strand at 1683.769 kb on + strand at 1683.822 kb on + strand, within RR42_RS28205 at 1683.880 kb on + strand, within RR42_RS28205 at 1684.417 kb on + strand, within RR42_RS28205 at 1684.417 kb on + strand, within RR42_RS28205 at 1684.430 kb on - strand, within RR42_RS28205 at 1684.430 kb on - strand, within RR42_RS28205 at 1684.488 kb on + strand, within RR42_RS28205 at 1684.690 kb on - strand, within RR42_RS28205 at 1684.690 kb on - strand, within RR42_RS28205 at 1684.786 kb on + strand, within RR42_RS28205 at 1684.940 kb on + strand, within RR42_RS28205 at 1684.966 kb on + strand, within RR42_RS28205 at 1684.967 kb on - strand, within RR42_RS28205 at 1684.970 kb on - strand, within RR42_RS28205 at 1684.972 kb on + strand, within RR42_RS28205 at 1684.972 kb on + strand, within RR42_RS28205 at 1684.973 kb on - strand, within RR42_RS28205 at 1685.014 kb on + strand, within RR42_RS28205 at 1685.229 kb on + strand, within RR42_RS28205 at 1685.229 kb on + strand, within RR42_RS28205 at 1685.229 kb on + strand, within RR42_RS28205 at 1685.252 kb on - strand, within RR42_RS28205 at 1685.263 kb on + strand, within RR42_RS28205 at 1685.299 kb on + strand, within RR42_RS28205 at 1685.300 kb on - strand, within RR42_RS28205 at 1685.347 kb on + strand, within RR42_RS28205 at 1685.347 kb on + strand, within RR42_RS28205 at 1685.347 kb on + strand, within RR42_RS28205 at 1685.348 kb on - strand, within RR42_RS28205 at 1685.362 kb on + strand, within RR42_RS28205 at 1685.363 kb on - strand, within RR42_RS28205 at 1685.515 kb on + strand at 1685.516 kb on - strand at 1685.587 kb on + strand at 1685.891 kb on + strand, within RR42_RS28210 at 1685.891 kb on + strand, within RR42_RS28210 at 1685.892 kb on - strand, within RR42_RS28210 at 1685.892 kb on - strand, within RR42_RS28210 at 1685.892 kb on - strand, within RR42_RS28210 at 1685.892 kb on - strand, within RR42_RS28210 at 1686.119 kb on + strand, within RR42_RS28210 at 1686.126 kb on - strand, within RR42_RS28210 at 1686.126 kb on - strand, within RR42_RS28210 at 1686.129 kb on + strand, within RR42_RS28210 at 1686.130 kb on - strand, within RR42_RS28210 at 1686.130 kb on - strand, within RR42_RS28210 at 1686.130 kb on - strand, within RR42_RS28210 at 1686.188 kb on + strand, within RR42_RS28210 at 1686.188 kb on + strand, within RR42_RS28210 at 1686.188 kb on + strand, within RR42_RS28210 at 1686.189 kb on - strand, within RR42_RS28210 at 1686.189 kb on - strand, within RR42_RS28210 at 1686.189 kb on - strand, within RR42_RS28210
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,682,701 - RR42_RS28195 0.78 -0.2 1,682,798 + RR42_RS28195 0.89 +0.3 1,682,799 + RR42_RS28195 0.89 -1.5 1,682,799 + RR42_RS28195 0.89 -0.6 1,682,908 + -0.6 1,682,909 - +0.4 1,683,121 + RR42_RS28200 0.44 -0.2 1,683,156 - RR42_RS28200 0.51 +0.1 1,683,285 + RR42_RS28200 0.76 -0.7 1,683,285 + RR42_RS28200 0.76 -1.0 1,683,286 - RR42_RS28200 0.76 +2.6 1,683,315 + RR42_RS28200 0.81 +0.2 1,683,316 - RR42_RS28200 0.82 -0.5 1,683,352 + RR42_RS28200 0.89 +0.4 1,683,503 + -0.1 1,683,504 - +0.4 1,683,508 + -0.4 1,683,510 + -1.4 1,683,511 - -0.4 1,683,549 + +0.2 1,683,580 - -1.3 1,683,688 - -1.0 1,683,751 + -1.3 1,683,751 + -0.7 1,683,769 + +1.6 1,683,822 + RR42_RS28205 0.11 +0.0 1,683,880 + RR42_RS28205 0.14 +0.8 1,684,417 + RR42_RS28205 0.39 +1.4 1,684,417 + RR42_RS28205 0.39 -0.4 1,684,430 - RR42_RS28205 0.40 +0.3 1,684,430 - RR42_RS28205 0.40 -0.3 1,684,488 + RR42_RS28205 0.42 -0.1 1,684,690 - RR42_RS28205 0.52 -1.2 1,684,690 - RR42_RS28205 0.52 -0.7 1,684,786 + RR42_RS28205 0.56 -1.6 1,684,940 + RR42_RS28205 0.64 -0.4 1,684,966 + RR42_RS28205 0.65 +0.0 1,684,967 - RR42_RS28205 0.65 -1.1 1,684,970 - RR42_RS28205 0.65 +1.0 1,684,972 + RR42_RS28205 0.65 -0.5 1,684,972 + RR42_RS28205 0.65 -1.0 1,684,973 - RR42_RS28205 0.65 -1.4 1,685,014 + RR42_RS28205 0.67 +1.4 1,685,229 + RR42_RS28205 0.77 -0.7 1,685,229 + RR42_RS28205 0.77 -1.2 1,685,229 + RR42_RS28205 0.77 +0.7 1,685,252 - RR42_RS28205 0.78 +0.5 1,685,263 + RR42_RS28205 0.79 -0.5 1,685,299 + RR42_RS28205 0.80 -0.1 1,685,300 - RR42_RS28205 0.80 +0.4 1,685,347 + RR42_RS28205 0.83 -0.3 1,685,347 + RR42_RS28205 0.83 -0.2 1,685,347 + RR42_RS28205 0.83 -0.9 1,685,348 - RR42_RS28205 0.83 +0.4 1,685,362 + RR42_RS28205 0.83 -1.0 1,685,363 - RR42_RS28205 0.83 -1.5 1,685,515 + +0.8 1,685,516 - +1.2 1,685,587 + -0.3 1,685,891 + RR42_RS28210 0.13 -0.4 1,685,891 + RR42_RS28210 0.13 -0.1 1,685,892 - RR42_RS28210 0.13 +0.7 1,685,892 - RR42_RS28210 0.13 -0.1 1,685,892 - RR42_RS28210 0.13 +0.6 1,685,892 - RR42_RS28210 0.13 +0.2 1,686,119 + RR42_RS28210 0.37 -0.2 1,686,126 - RR42_RS28210 0.38 +0.2 1,686,126 - RR42_RS28210 0.38 +1.3 1,686,129 + RR42_RS28210 0.38 +0.8 1,686,130 - RR42_RS28210 0.39 +0.8 1,686,130 - RR42_RS28210 0.39 -0.2 1,686,130 - RR42_RS28210 0.39 +0.1 1,686,188 + RR42_RS28210 0.45 -0.6 1,686,188 + RR42_RS28210 0.45 -2.6 1,686,188 + RR42_RS28210 0.45 +1.0 1,686,189 - RR42_RS28210 0.45 -1.0 1,686,189 - RR42_RS28210 0.45 +0.0 1,686,189 - RR42_RS28210 0.45 -0.4
Or see this region's nucleotide sequence