Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27685 and RR42_RS27690 are separated by 35 nucleotides RR42_RS27690 and RR42_RS27695 are separated by 238 nucleotides
RR42_RS27685: RR42_RS27685 - multidrug efflux RND transporter permease subunit, at 1,573,569 to 1,576,760
_RS27685
RR42_RS27690: RR42_RS27690 - RND transporter, at 1,576,796 to 1,578,319
_RS27690
RR42_RS27695: RR42_RS27695 - polyketide cyclase, at 1,578,558 to 1,579,103
_RS27695
Position (kb)
1576
1577
1578
1579 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1575.847 kb on - strand, within RR42_RS27685 at 1575.861 kb on + strand, within RR42_RS27685 at 1575.862 kb on - strand, within RR42_RS27685 at 1575.894 kb on + strand, within RR42_RS27685 at 1575.895 kb on - strand, within RR42_RS27685 at 1576.340 kb on - strand, within RR42_RS27685 at 1576.340 kb on - strand, within RR42_RS27685 at 1576.665 kb on + strand at 1576.665 kb on + strand at 1576.666 kb on - strand at 1576.666 kb on - strand at 1576.758 kb on + strand at 1576.758 kb on + strand at 1576.759 kb on - strand at 1576.807 kb on - strand at 1576.883 kb on + strand at 1576.923 kb on - strand at 1577.261 kb on + strand, within RR42_RS27690 at 1577.262 kb on - strand, within RR42_RS27690 at 1577.777 kb on + strand, within RR42_RS27690 at 1578.028 kb on - strand, within RR42_RS27690 at 1578.110 kb on + strand, within RR42_RS27690 at 1578.131 kb on + strand, within RR42_RS27690 at 1578.132 kb on - strand, within RR42_RS27690 at 1578.157 kb on + strand, within RR42_RS27690 at 1578.158 kb on - strand, within RR42_RS27690 at 1578.158 kb on - strand, within RR42_RS27690 at 1578.412 kb on + strand at 1578.413 kb on - strand at 1578.417 kb on + strand at 1578.417 kb on + strand at 1578.417 kb on + strand at 1578.417 kb on + strand at 1578.417 kb on + strand at 1578.418 kb on - strand at 1578.418 kb on - strand at 1578.418 kb on - strand at 1578.641 kb on + strand, within RR42_RS27695 at 1578.691 kb on - strand, within RR42_RS27695 at 1578.912 kb on + strand, within RR42_RS27695 at 1578.913 kb on - strand, within RR42_RS27695 at 1579.178 kb on - strand at 1579.178 kb on - strand at 1579.293 kb on + strand at 1579.296 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,575,847 - RR42_RS27685 0.71 +0.3 1,575,861 + RR42_RS27685 0.72 -0.2 1,575,862 - RR42_RS27685 0.72 -1.4 1,575,894 + RR42_RS27685 0.73 -0.7 1,575,895 - RR42_RS27685 0.73 +0.1 1,576,340 - RR42_RS27685 0.87 -0.3 1,576,340 - RR42_RS27685 0.87 -0.3 1,576,665 + -1.2 1,576,665 + -0.2 1,576,666 - -0.1 1,576,666 - +1.0 1,576,758 + -0.2 1,576,758 + -1.0 1,576,759 - +1.0 1,576,807 - +0.3 1,576,883 + -0.4 1,576,923 - +1.7 1,577,261 + RR42_RS27690 0.31 -0.2 1,577,262 - RR42_RS27690 0.31 -0.1 1,577,777 + RR42_RS27690 0.64 -0.5 1,578,028 - RR42_RS27690 0.81 +3.2 1,578,110 + RR42_RS27690 0.86 +0.6 1,578,131 + RR42_RS27690 0.88 -0.2 1,578,132 - RR42_RS27690 0.88 -2.2 1,578,157 + RR42_RS27690 0.89 -0.1 1,578,158 - RR42_RS27690 0.89 +0.3 1,578,158 - RR42_RS27690 0.89 +1.3 1,578,412 + -0.9 1,578,413 - -1.2 1,578,417 + +1.7 1,578,417 + +0.6 1,578,417 + +0.7 1,578,417 + +1.2 1,578,417 + +1.1 1,578,418 - -0.5 1,578,418 - +0.8 1,578,418 - -2.0 1,578,641 + RR42_RS27695 0.15 -1.0 1,578,691 - RR42_RS27695 0.24 -1.5 1,578,912 + RR42_RS27695 0.65 +0.5 1,578,913 - RR42_RS27695 0.65 +0.1 1,579,178 - -0.2 1,579,178 - +0.3 1,579,293 + -0.6 1,579,296 - -1.5
Or see this region's nucleotide sequence