Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27105 and RR42_RS27110 are separated by 45 nucleotides RR42_RS27110 and RR42_RS27115 are separated by 264 nucleotides RR42_RS27115 and RR42_RS27120 overlap by 20 nucleotides RR42_RS27120 and RR42_RS27125 overlap by 4 nucleotides
RR42_RS27105: RR42_RS27105 - hypothetical protein, at 1,435,127 to 1,435,360
_RS27105
RR42_RS27110: RR42_RS27110 - hypothetical protein, at 1,435,406 to 1,435,720
_RS27110
RR42_RS27115: RR42_RS27115 - amino acid ABC transporter ATP-binding protein, at 1,435,985 to 1,436,713
_RS27115
RR42_RS27120: RR42_RS27120 - amino acid ABC transporter permease, at 1,436,694 to 1,437,350
_RS27120
RR42_RS27125: RR42_RS27125 - polar amino acid ABC transporter permease, at 1,437,347 to 1,438,012
_RS27125
Position (kb)
1435
1436
1437 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1435.060 kb on + strand at 1435.061 kb on - strand at 1435.139 kb on + strand at 1435.218 kb on + strand, within RR42_RS27105 at 1435.377 kb on + strand at 1435.377 kb on + strand at 1435.378 kb on - strand at 1435.521 kb on + strand, within RR42_RS27110 at 1435.522 kb on - strand, within RR42_RS27110 at 1435.522 kb on - strand, within RR42_RS27110 at 1435.522 kb on - strand, within RR42_RS27110 at 1435.697 kb on + strand at 1435.698 kb on - strand at 1435.791 kb on - strand at 1435.791 kb on - strand at 1435.801 kb on + strand at 1435.801 kb on + strand at 1435.825 kb on + strand at 1435.825 kb on + strand at 1435.826 kb on - strand at 1435.839 kb on + strand at 1435.839 kb on + strand at 1435.839 kb on + strand at 1435.840 kb on - strand at 1435.846 kb on - strand at 1436.231 kb on + strand, within RR42_RS27115 at 1436.232 kb on - strand, within RR42_RS27115 at 1436.232 kb on - strand, within RR42_RS27115 at 1436.232 kb on - strand, within RR42_RS27115 at 1436.232 kb on - strand, within RR42_RS27115 at 1436.536 kb on + strand, within RR42_RS27115 at 1436.569 kb on + strand, within RR42_RS27115 at 1436.954 kb on - strand, within RR42_RS27120 at 1437.420 kb on + strand, within RR42_RS27125 at 1437.420 kb on + strand, within RR42_RS27125 at 1437.420 kb on + strand, within RR42_RS27125 at 1437.421 kb on - strand, within RR42_RS27125
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,435,060 + -0.2 1,435,061 - +0.2 1,435,139 + -0.2 1,435,218 + RR42_RS27105 0.39 -0.0 1,435,377 + -2.0 1,435,377 + +0.3 1,435,378 - -0.1 1,435,521 + RR42_RS27110 0.37 -0.2 1,435,522 - RR42_RS27110 0.37 -1.4 1,435,522 - RR42_RS27110 0.37 +0.7 1,435,522 - RR42_RS27110 0.37 -1.1 1,435,697 + -1.9 1,435,698 - +1.5 1,435,791 - -1.4 1,435,791 - +0.8 1,435,801 + +0.1 1,435,801 + -1.2 1,435,825 + +0.6 1,435,825 + +0.0 1,435,826 - -0.2 1,435,839 + -0.7 1,435,839 + -0.4 1,435,839 + -1.1 1,435,840 - -0.7 1,435,846 - -0.6 1,436,231 + RR42_RS27115 0.34 +1.4 1,436,232 - RR42_RS27115 0.34 +0.5 1,436,232 - RR42_RS27115 0.34 +0.8 1,436,232 - RR42_RS27115 0.34 +1.8 1,436,232 - RR42_RS27115 0.34 +0.8 1,436,536 + RR42_RS27115 0.76 -0.8 1,436,569 + RR42_RS27115 0.80 +0.4 1,436,954 - RR42_RS27120 0.40 -2.7 1,437,420 + RR42_RS27125 0.11 -1.3 1,437,420 + RR42_RS27125 0.11 +0.8 1,437,420 + RR42_RS27125 0.11 -1.1 1,437,421 - RR42_RS27125 0.11 +0.3
Or see this region's nucleotide sequence